Population Structure Based on Microsatellite Length Polymorphism, Antifungal Susceptibility Profile, and Enzymatic Activity of <i>Candida auris</i> Clinical Isolates in Russia
<i>Candida auris</i> is an emerging multidrug-resistant fungal pathogen causing nosocomial transmission and invasive infections with high mortality. This study aimed to investigate the genetic relationships, enzymatic activities, and drug-resistance profiles of <i>C. auris</i>...
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2025-01-01
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| author | Ellina Oganesyan Victoria Klimenteva Irina Vybornova Valentina Venchakova Ekaterina Parshikova Sergey Kovyrshin Olga Orlova Alexander Kruglov Svetlana Gordeeva Natalya Vasilyeva Anastasiya Taraskina |
| author_facet | Ellina Oganesyan Victoria Klimenteva Irina Vybornova Valentina Venchakova Ekaterina Parshikova Sergey Kovyrshin Olga Orlova Alexander Kruglov Svetlana Gordeeva Natalya Vasilyeva Anastasiya Taraskina |
| author_sort | Ellina Oganesyan |
| collection | DOAJ |
| description | <i>Candida auris</i> is an emerging multidrug-resistant fungal pathogen causing nosocomial transmission and invasive infections with high mortality. This study aimed to investigate the genetic relationships, enzymatic activities, and drug-resistance profiles of <i>C. auris</i> isolates to evaluate the population and epidemiological diversity of candidiasis in Russia. A total of 112 clinical isolates of <i>C. auris</i> were analyzed from May 2017 to March 2023 in 18 hospitals across Saint Petersburg, the Leningrad Region, and Moscow. Species identification was confirmed by ITS sequencing, and genotyping was performed using 12 short tandem repeat (STR) markers. Antifungal susceptibility was tested using Sensititre™ YeastOne™ plates, and hydrolytic enzyme production was measured by the plate method. ITS sequencing confirmed that all isolates belonged to a single ITS cluster (clades I and III). Fifteen distinct STR genotypes were identified, with genotype I being dominant (<i>n</i> = 53). The most variable of the analyzed markers turned out to be M3-Ia, which was represented in the Russian population by eight different variants. Fluconazole resistance was found in 111 isolates, 17% were resistant to amphotericin B, and 3.6% to 5-flucytosine. Phospholipase activity was strong in most strains, especially in urine isolates (<i>p</i> = 0.014). Conclusion: The predominance of STR genotype I and its variability at the M3-Ia locus suggest its association with nosocomial outbreaks and transmissibility in Russia. |
| format | Article |
| id | doaj-art-043f2ad67e5443be99c7d12e21930054 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj-art-043f2ad67e5443be99c7d12e219300542025-01-24T13:37:18ZengMDPI AGJournal of Fungi2309-608X2025-01-011113510.3390/jof11010035Population Structure Based on Microsatellite Length Polymorphism, Antifungal Susceptibility Profile, and Enzymatic Activity of <i>Candida auris</i> Clinical Isolates in RussiaEllina Oganesyan0Victoria Klimenteva1Irina Vybornova2Valentina Venchakova3Ekaterina Parshikova4Sergey Kovyrshin5Olga Orlova6Alexander Kruglov7Svetlana Gordeeva8Natalya Vasilyeva9Anastasiya Taraskina10Kashkin Research Institute of Medical Mycology, North-Western State Medical University Named after I.I. Mechnikov, 191015 Saint Petersburg, RussiaKashkin Research Institute of Medical Mycology, North-Western State Medical University Named after I.I. Mechnikov, 191015 Saint Petersburg, RussiaKashkin Research Institute of Medical Mycology, North-Western State Medical University Named after I.I. Mechnikov, 191015 Saint Petersburg, RussiaDepartment of Medical Microbiology, North-Western State Medical University Named after I.I. Mechnikov, 191015 Saint Petersburg, RussiaDepartment of Medical Microbiology, North-Western State Medical University Named after I.I. Mechnikov, 191015 Saint Petersburg, RussiaKashkin Research Institute of Medical Mycology, North-Western State Medical University Named after I.I. Mechnikov, 191015 Saint Petersburg, RussiaMoscow L.A. Vorokhobov Municipal Clinical Hospital № 67, 123423 Moscow, RussiaMoscow Municipal Clinical Hospital № 40, 129301 Moscow, RussiaClinical Infectious Diseases Hospital Named after S.P. Botkin, 195067 Saint Petersburg, RussiaKashkin Research Institute of Medical Mycology, North-Western State Medical University Named after I.I. Mechnikov, 191015 Saint Petersburg, RussiaKashkin Research Institute of Medical Mycology, North-Western State Medical University Named after I.I. Mechnikov, 191015 Saint Petersburg, Russia<i>Candida auris</i> is an emerging multidrug-resistant fungal pathogen causing nosocomial transmission and invasive infections with high mortality. This study aimed to investigate the genetic relationships, enzymatic activities, and drug-resistance profiles of <i>C. auris</i> isolates to evaluate the population and epidemiological diversity of candidiasis in Russia. A total of 112 clinical isolates of <i>C. auris</i> were analyzed from May 2017 to March 2023 in 18 hospitals across Saint Petersburg, the Leningrad Region, and Moscow. Species identification was confirmed by ITS sequencing, and genotyping was performed using 12 short tandem repeat (STR) markers. Antifungal susceptibility was tested using Sensititre™ YeastOne™ plates, and hydrolytic enzyme production was measured by the plate method. ITS sequencing confirmed that all isolates belonged to a single ITS cluster (clades I and III). Fifteen distinct STR genotypes were identified, with genotype I being dominant (<i>n</i> = 53). The most variable of the analyzed markers turned out to be M3-Ia, which was represented in the Russian population by eight different variants. Fluconazole resistance was found in 111 isolates, 17% were resistant to amphotericin B, and 3.6% to 5-flucytosine. Phospholipase activity was strong in most strains, especially in urine isolates (<i>p</i> = 0.014). Conclusion: The predominance of STR genotype I and its variability at the M3-Ia locus suggest its association with nosocomial outbreaks and transmissibility in Russia.https://www.mdpi.com/2309-608X/11/1/35<i>Candida auris</i>nosocomial outbreakmolecular epidemiologymicrosatellite length polymorphismantifungal susceptibilityphospholipase activity |
| spellingShingle | Ellina Oganesyan Victoria Klimenteva Irina Vybornova Valentina Venchakova Ekaterina Parshikova Sergey Kovyrshin Olga Orlova Alexander Kruglov Svetlana Gordeeva Natalya Vasilyeva Anastasiya Taraskina Population Structure Based on Microsatellite Length Polymorphism, Antifungal Susceptibility Profile, and Enzymatic Activity of <i>Candida auris</i> Clinical Isolates in Russia Journal of Fungi <i>Candida auris</i> nosocomial outbreak molecular epidemiology microsatellite length polymorphism antifungal susceptibility phospholipase activity |
| title | Population Structure Based on Microsatellite Length Polymorphism, Antifungal Susceptibility Profile, and Enzymatic Activity of <i>Candida auris</i> Clinical Isolates in Russia |
| title_full | Population Structure Based on Microsatellite Length Polymorphism, Antifungal Susceptibility Profile, and Enzymatic Activity of <i>Candida auris</i> Clinical Isolates in Russia |
| title_fullStr | Population Structure Based on Microsatellite Length Polymorphism, Antifungal Susceptibility Profile, and Enzymatic Activity of <i>Candida auris</i> Clinical Isolates in Russia |
| title_full_unstemmed | Population Structure Based on Microsatellite Length Polymorphism, Antifungal Susceptibility Profile, and Enzymatic Activity of <i>Candida auris</i> Clinical Isolates in Russia |
| title_short | Population Structure Based on Microsatellite Length Polymorphism, Antifungal Susceptibility Profile, and Enzymatic Activity of <i>Candida auris</i> Clinical Isolates in Russia |
| title_sort | population structure based on microsatellite length polymorphism antifungal susceptibility profile and enzymatic activity of i candida auris i clinical isolates in russia |
| topic | <i>Candida auris</i> nosocomial outbreak molecular epidemiology microsatellite length polymorphism antifungal susceptibility phospholipase activity |
| url | https://www.mdpi.com/2309-608X/11/1/35 |
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