Assessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma
Abstract Introduction Pancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis. The roles of the transcription factor special AT-rich binding protein-2 (SATB2) and β-catenin in PDAC have been a subject of controversy. We aimed to assess the diagnostic and prognostic impact of SATB2...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Journal of Cancer Research and Clinical Oncology |
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| Online Access: | https://doi.org/10.1007/s00432-024-06055-z |
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| author | Noha Said Helal Sara Maher Safia Samir Hesham A. Elmeligy Mohammed A. Aboul-Ezz Tarek Aboushousha Mona Moussa |
| author_facet | Noha Said Helal Sara Maher Safia Samir Hesham A. Elmeligy Mohammed A. Aboul-Ezz Tarek Aboushousha Mona Moussa |
| author_sort | Noha Said Helal |
| collection | DOAJ |
| description | Abstract Introduction Pancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis. The roles of the transcription factor special AT-rich binding protein-2 (SATB2) and β-catenin in PDAC have been a subject of controversy. We aimed to assess the diagnostic and prognostic impact of SATB2 and β-catenin in PDAC. Methods We analyzed 44 paraffin-embedded tissue blocks along with corresponding blood and pancreatic tissues. We evaluated SATB2 expression using immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA). β-catenin was assessed using IHC and real-time polymerase chain reaction (qPCR). Results High SATB2 expression and low β-catenin expression were associated with a poor prognosis in PDAC, including advanced pathological tumor stage (pT-stage), pathological lymph node stage (pN-stage), and TNM stage. We found a positive correlation between SATB2 expression assessed by IHC and the concentration of SATB2 in both serum and tissue samples measured by ELISA. We observed a positive correlation between β-catenin expression assessed by IHC and β-catenin levels measured by qPCR. Conclusions SATB2 and β-catenin could provide valuable insights into the development of pancreatic cancer, and targeting them may be beneficial for the prevention and treatment of PDAC. The levels of SATB2 in serum show promise for the diagnosis and tumor invasion of pancreatic cancer. |
| format | Article |
| id | doaj-art-042e1b92b43f41279fc8723747d8c3e1 |
| institution | Kabale University |
| issn | 1432-1335 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Cancer Research and Clinical Oncology |
| spelling | doaj-art-042e1b92b43f41279fc8723747d8c3e12025-02-02T12:07:16ZengSpringerJournal of Cancer Research and Clinical Oncology1432-13352025-01-01151211110.1007/s00432-024-06055-zAssessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinomaNoha Said Helal0Sara Maher1Safia Samir2Hesham A. Elmeligy3Mohammed A. Aboul-Ezz4Tarek Aboushousha5Mona Moussa6Department of Pathology, Theodor Bilharz Research InstituteDepartment of Immunology, Theodor Bilharz Research InstituteDepartment of Biochemistry and Molecular Biology, Theodor Bilharz Research InstituteDepartment of Surgery, Theodor Bilharz Research InstituteDepartment of Hepatology and Gastroenterology, Theodor Bilharz Research InstituteDepartment of Pathology, Theodor Bilharz Research InstituteDepartment of Pathology, Theodor Bilharz Research InstituteAbstract Introduction Pancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis. The roles of the transcription factor special AT-rich binding protein-2 (SATB2) and β-catenin in PDAC have been a subject of controversy. We aimed to assess the diagnostic and prognostic impact of SATB2 and β-catenin in PDAC. Methods We analyzed 44 paraffin-embedded tissue blocks along with corresponding blood and pancreatic tissues. We evaluated SATB2 expression using immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA). β-catenin was assessed using IHC and real-time polymerase chain reaction (qPCR). Results High SATB2 expression and low β-catenin expression were associated with a poor prognosis in PDAC, including advanced pathological tumor stage (pT-stage), pathological lymph node stage (pN-stage), and TNM stage. We found a positive correlation between SATB2 expression assessed by IHC and the concentration of SATB2 in both serum and tissue samples measured by ELISA. We observed a positive correlation between β-catenin expression assessed by IHC and β-catenin levels measured by qPCR. Conclusions SATB2 and β-catenin could provide valuable insights into the development of pancreatic cancer, and targeting them may be beneficial for the prevention and treatment of PDAC. The levels of SATB2 in serum show promise for the diagnosis and tumor invasion of pancreatic cancer.https://doi.org/10.1007/s00432-024-06055-zPancreatic ductal adenocarcinomaSATB2β-cateninIHCELISAqPCR |
| spellingShingle | Noha Said Helal Sara Maher Safia Samir Hesham A. Elmeligy Mohammed A. Aboul-Ezz Tarek Aboushousha Mona Moussa Assessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma Journal of Cancer Research and Clinical Oncology Pancreatic ductal adenocarcinoma SATB2 β-catenin IHC ELISA qPCR |
| title | Assessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma |
| title_full | Assessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma |
| title_fullStr | Assessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma |
| title_full_unstemmed | Assessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma |
| title_short | Assessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma |
| title_sort | assessing the diagnostic potential of satb2 and β catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma |
| topic | Pancreatic ductal adenocarcinoma SATB2 β-catenin IHC ELISA qPCR |
| url | https://doi.org/10.1007/s00432-024-06055-z |
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