Widespread inter‐individual gene expression variability in Arabidopsis thaliana

Abstract A fundamental question in biology is how gene expression is regulated to give rise to a phenotype. However, transcriptional variability is rarely considered although it could influence the relationship between genotype and phenotype. It is known in unicellular organisms that gene expression...

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Main Authors: Sandra Cortijo, Zeynep Aydin, Sebastian Ahnert, James CW Locke
Format: Article
Language:English
Published: Springer Nature 2019-01-01
Series:Molecular Systems Biology
Subjects:
Online Access:https://doi.org/10.15252/msb.20188591
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author Sandra Cortijo
Zeynep Aydin
Sebastian Ahnert
James CW Locke
author_facet Sandra Cortijo
Zeynep Aydin
Sebastian Ahnert
James CW Locke
author_sort Sandra Cortijo
collection DOAJ
description Abstract A fundamental question in biology is how gene expression is regulated to give rise to a phenotype. However, transcriptional variability is rarely considered although it could influence the relationship between genotype and phenotype. It is known in unicellular organisms that gene expression is often noisy rather than uniform, and this has been proposed to be beneficial when environmental conditions are unpredictable. However, little is known about inter‐individual transcriptional variability in multicellular organisms. Using transcriptomic approaches, we analysed gene expression variability between individual Arabidopsis thaliana plants growing in identical conditions over a 24‐h time course. We identified hundreds of genes that exhibit high inter‐individual variability and found that many are involved in environmental responses, with different classes of genes variable between the day and night. We also identified factors that might facilitate gene expression variability, such as gene length, the number of transcription factors regulating the genes and the chromatin environment. These results shed new light on the impact of transcriptional variability in gene expression regulation in plants.
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issn 1744-4292
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publishDate 2019-01-01
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record_format Article
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spelling doaj-art-04089d5147bb447aa4f9f98bcfe11a8f2025-08-20T03:06:42ZengSpringer NatureMolecular Systems Biology1744-42922019-01-0115111610.15252/msb.20188591Widespread inter‐individual gene expression variability in Arabidopsis thalianaSandra Cortijo0Zeynep Aydin1Sebastian Ahnert2James CW Locke3The Sainsbury Laboratory, University of CambridgeThe Sainsbury Laboratory, University of CambridgeThe Sainsbury Laboratory, University of CambridgeThe Sainsbury Laboratory, University of CambridgeAbstract A fundamental question in biology is how gene expression is regulated to give rise to a phenotype. However, transcriptional variability is rarely considered although it could influence the relationship between genotype and phenotype. It is known in unicellular organisms that gene expression is often noisy rather than uniform, and this has been proposed to be beneficial when environmental conditions are unpredictable. However, little is known about inter‐individual transcriptional variability in multicellular organisms. Using transcriptomic approaches, we analysed gene expression variability between individual Arabidopsis thaliana plants growing in identical conditions over a 24‐h time course. We identified hundreds of genes that exhibit high inter‐individual variability and found that many are involved in environmental responses, with different classes of genes variable between the day and night. We also identified factors that might facilitate gene expression variability, such as gene length, the number of transcription factors regulating the genes and the chromatin environment. These results shed new light on the impact of transcriptional variability in gene expression regulation in plants.https://doi.org/10.15252/msb.20188591Arabidopsis thalianainter‐individual heterogeneitynoise in gene expressionRNA‐seqtranscriptional variability
spellingShingle Sandra Cortijo
Zeynep Aydin
Sebastian Ahnert
James CW Locke
Widespread inter‐individual gene expression variability in Arabidopsis thaliana
Molecular Systems Biology
Arabidopsis thaliana
inter‐individual heterogeneity
noise in gene expression
RNA‐seq
transcriptional variability
title Widespread inter‐individual gene expression variability in Arabidopsis thaliana
title_full Widespread inter‐individual gene expression variability in Arabidopsis thaliana
title_fullStr Widespread inter‐individual gene expression variability in Arabidopsis thaliana
title_full_unstemmed Widespread inter‐individual gene expression variability in Arabidopsis thaliana
title_short Widespread inter‐individual gene expression variability in Arabidopsis thaliana
title_sort widespread inter individual gene expression variability in arabidopsis thaliana
topic Arabidopsis thaliana
inter‐individual heterogeneity
noise in gene expression
RNA‐seq
transcriptional variability
url https://doi.org/10.15252/msb.20188591
work_keys_str_mv AT sandracortijo widespreadinterindividualgeneexpressionvariabilityinarabidopsisthaliana
AT zeynepaydin widespreadinterindividualgeneexpressionvariabilityinarabidopsisthaliana
AT sebastianahnert widespreadinterindividualgeneexpressionvariabilityinarabidopsisthaliana
AT jamescwlocke widespreadinterindividualgeneexpressionvariabilityinarabidopsisthaliana