Reverse vaccinology-based multi-epitope COVID-19 vaccine targeting SARS-CoV-2 structural and non-structural proteins induces immune responses in mice

Reverse vaccinology-based multi-epitope COVID-19 vaccine targeting SARS-CoV-2 structural and non-structural proteins induces immune responses in mice

Vaccination is effective to end pandemics, including the Coronavirus disease-2019 (COVID-19). However, the evolution of Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) which is characterized by the emergence of the virus variants has a great potential to cause a decrease in the effectiv...

Full description

Saved in:
Bibliographic Details
Main Authors: Azzania Fibriani, Nicholas Yamahoki, Annisa Meliana Shani, Afifatur Rofiqoh, Agust Leo Fany Siregar, Cynthia Gunawan, Carola Giovanni Danira Mayorga, Tommy Octavianus Soetrisno Tjia, Husna Nugrahapraja, Ernawati Arifin Giri-Rachman, Marselina Irasonia Tan
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Vaccine: X
Subjects:
SARS-CoV-2
Recombinant vaccine
Peptide-based vaccine
Multi-epitope vaccine
Reverse vaccinology
Immunogenicity
Online Access:http://www.sciencedirect.com/science/article/pii/S2590136225000269
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vaccination is effective to end pandemics, including the Coronavirus disease-2019 (COVID-19). However, the evolution of Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) which is characterized by the emergence of the virus variants has a great potential to cause a decrease in the effectiveness of the developed vaccines. One of the vaccine platforms that may overcome vaccine failure due to viral genetic mutations is a multi-epitope vaccine. Using the reverse vaccinology approach, in this study, we developed a multi-epitope peptide-based COVID-19 vaccine composed of immunodominant epitopes. The multi-epitope peptide was designed in silico, successfully expressed in E. coli BL21 (DE3), and purified. Furthermore, the vaccine candidate was proven to induce the production of SARS-CoV-2 antigen-specific IgM and IgG antibodies in BALB/c mice without any considerable adverse reaction. The results also showed that the generated antibodies were reactive to SARS-CoV-2-positive patient nasopharyngeal swab samples containing different circulating Omicron XBB F456L variants. Therefore, our study demonstrates that the multi-epitope peptide has the potential to be further developed as a safe and relevant COVID-19 vaccine. Additionally, this study also demonstrates that reverse vaccinology is useful to facilitate the development of relevant vaccines for emerging infectious diseases.
ISSN:2590-1362

Similar Items