Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility
Aims. The programmed death- (PD-) 1/PD-1 ligand (PD-L) pathway plays an important role in regulating T cell activation and maintaining peripheral tolerance. Accumulated studies showed that PD-1/PD-L1 pathway was involved in the development of type 1 diabetes (T1DM). Since the genetic background of t...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2018/1614683 |
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| author | Chenyue Qian Heming Guo Xiaohong Chen Aiming Shi Sicheng Li Xin Wang Jie Pan Chen Fang |
| author_facet | Chenyue Qian Heming Guo Xiaohong Chen Aiming Shi Sicheng Li Xin Wang Jie Pan Chen Fang |
| author_sort | Chenyue Qian |
| collection | DOAJ |
| description | Aims. The programmed death- (PD-) 1/PD-1 ligand (PD-L) pathway plays an important role in regulating T cell activation and maintaining peripheral tolerance. Accumulated studies showed that PD-1/PD-L1 pathway was involved in the development of type 1 diabetes (T1DM). Since the genetic background of type 1 diabetes differs greatly among the different population, we aim to investigate the association of genetic polymorphisms in PD-1 and PD-L1 with T1DM susceptibility in Chinese population. Methods. In total, 166 T1DM patients and 100 healthy controls were enrolled into the study. Genomic DNA was extracted from 4 mL peripheral blood samples collected from each subject. Genotyping of 8 selected SNPs of PD-1 and PD-L1 was carried out by the pyrosequencing PSQ 24 System using PyroMark Gold reagents (QIAGEN). Results. SNP rs4143815 in PD-L1 was significantly associated with T1DM. People carrying the C allele of rs4143815 suffering less risk of T1DM and T1DM patients with G/G genotype showed higher levels of autoantibody (AAB) positive incidence compared with C allele carriers. No significant associations were found in other SNPs. Conclusions. Our results indicate that rs4143815 of PD-L1 is significantly associated with T1DM and may serve as a new biomarker to predict the T1DM susceptibility. |
| format | Article |
| id | doaj-art-03c86fcd9bac44afb8871cba49d05325 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-03c86fcd9bac44afb8871cba49d053252025-02-03T01:25:32ZengWileyJournal of Diabetes Research2314-67452314-67532018-01-01201810.1155/2018/16146831614683Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes SusceptibilityChenyue Qian0Heming Guo1Xiaohong Chen2Aiming Shi3Sicheng Li4Xin Wang5Jie Pan6Chen Fang7Department of Pharmacy, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaDepartment of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaDepartment of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaDepartment of Pharmacy, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaDepartment of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaDepartment of Endocrinology, Jiangsu Province Hospital of TCM, 155 Hanzhonglu, Jiangsu Nanjing 210029, ChinaDepartment of Pharmacy, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaDepartment of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaAims. The programmed death- (PD-) 1/PD-1 ligand (PD-L) pathway plays an important role in regulating T cell activation and maintaining peripheral tolerance. Accumulated studies showed that PD-1/PD-L1 pathway was involved in the development of type 1 diabetes (T1DM). Since the genetic background of type 1 diabetes differs greatly among the different population, we aim to investigate the association of genetic polymorphisms in PD-1 and PD-L1 with T1DM susceptibility in Chinese population. Methods. In total, 166 T1DM patients and 100 healthy controls were enrolled into the study. Genomic DNA was extracted from 4 mL peripheral blood samples collected from each subject. Genotyping of 8 selected SNPs of PD-1 and PD-L1 was carried out by the pyrosequencing PSQ 24 System using PyroMark Gold reagents (QIAGEN). Results. SNP rs4143815 in PD-L1 was significantly associated with T1DM. People carrying the C allele of rs4143815 suffering less risk of T1DM and T1DM patients with G/G genotype showed higher levels of autoantibody (AAB) positive incidence compared with C allele carriers. No significant associations were found in other SNPs. Conclusions. Our results indicate that rs4143815 of PD-L1 is significantly associated with T1DM and may serve as a new biomarker to predict the T1DM susceptibility.http://dx.doi.org/10.1155/2018/1614683 |
| spellingShingle | Chenyue Qian Heming Guo Xiaohong Chen Aiming Shi Sicheng Li Xin Wang Jie Pan Chen Fang Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility Journal of Diabetes Research |
| title | Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility |
| title_full | Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility |
| title_fullStr | Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility |
| title_full_unstemmed | Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility |
| title_short | Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility |
| title_sort | association of pd 1 and pd l1 genetic polymorphyisms with type 1 diabetes susceptibility |
| url | http://dx.doi.org/10.1155/2018/1614683 |
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