Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosis
Nitroreductase (NTR) plays a critical role in the oxygen-deficient environment of anoxic tumor cells, and its identification is crucial for the diagnosis and treatment of cancer. This research introduces an innovative Surface Enhanced Raman Scattering (SERS) probe, created by attaching p-nitrothioph...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | SLAS Technology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2472630324001110 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832590859424497664 |
|---|---|
| author | Xiaoyue Zhao Ying Zhang Chunyan Zhu Zhihui Yang Xiaoyuan Chu |
| author_facet | Xiaoyue Zhao Ying Zhang Chunyan Zhu Zhihui Yang Xiaoyuan Chu |
| author_sort | Xiaoyue Zhao |
| collection | DOAJ |
| description | Nitroreductase (NTR) plays a critical role in the oxygen-deficient environment of anoxic tumor cells, and its identification is crucial for the diagnosis and treatment of cancer. This research introduces an innovative Surface Enhanced Raman Scattering (SERS) probe, created by attaching p-nitrothiophenol (p-NTP) to gold nanoparticles (Au NPs). This probe leverages the specific enzymatic reaction of NTR in hypoxic status, utilizing decreased NADH. The enzymatic activity of NTR transforms nitroaromatic compounds into aromatic amines, which is then reflected as a measurable shift in the SERS signal of the probe. This novel approach allows for the accurate quantification of NTR, with the sensitivity reaching a detection threshold of less than 0.02 μg/mL. The probe's non-toxic nature and superior biocompatibility facilitate its use for direct SERS investigations in A549 cells under reduced oxygen levels. We also applied this method to xenograft model. The results demonstrate a marked increase in NTR levels in tumor cells and tumor tissues in hypoxic conditions, highlighting the significance of this nanoprobe in enhancing cancer diagnostics, helping medical doctors making treatment decisions more swiftly and effectively. |
| format | Article |
| id | doaj-art-03c6d182dc7548e5b8b2c9f0fe2092e2 |
| institution | Kabale University |
| issn | 2472-6303 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | SLAS Technology |
| spelling | doaj-art-03c6d182dc7548e5b8b2c9f0fe2092e22025-01-23T05:27:27ZengElsevierSLAS Technology2472-63032025-02-0130100229Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosisXiaoyue Zhao0Ying Zhang1Chunyan Zhu2Zhihui Yang3Xiaoyuan Chu4Department of Medical Oncology, Affiliated Jinling Hospital of Medical School of Nanjing University, Nanjing 210000, PR ChinaDepartment of Pathology, Affiliated Jinling Hospital of Medical School of Nanjing University, Nanjing 210000, PR ChinaDepartment of Medical Oncology, Affiliated Jinling Hospital of Medical School of Nanjing University, Nanjing 210000, PR ChinaDepartment of Pathology, Affiliated Jinling Hospital of Medical School of Nanjing University, Nanjing 210000, PR ChinaDepartment of Medical Oncology, Affiliated Jinling Hospital of Medical School of Nanjing University, Nanjing 210000, PR China; Corresponding author at: Affiliated Jinling Hospital of Medical School of Nanjing University, #305 Zhongshan East Road, Nanjing 210000, PR China.Nitroreductase (NTR) plays a critical role in the oxygen-deficient environment of anoxic tumor cells, and its identification is crucial for the diagnosis and treatment of cancer. This research introduces an innovative Surface Enhanced Raman Scattering (SERS) probe, created by attaching p-nitrothiophenol (p-NTP) to gold nanoparticles (Au NPs). This probe leverages the specific enzymatic reaction of NTR in hypoxic status, utilizing decreased NADH. The enzymatic activity of NTR transforms nitroaromatic compounds into aromatic amines, which is then reflected as a measurable shift in the SERS signal of the probe. This novel approach allows for the accurate quantification of NTR, with the sensitivity reaching a detection threshold of less than 0.02 μg/mL. The probe's non-toxic nature and superior biocompatibility facilitate its use for direct SERS investigations in A549 cells under reduced oxygen levels. We also applied this method to xenograft model. The results demonstrate a marked increase in NTR levels in tumor cells and tumor tissues in hypoxic conditions, highlighting the significance of this nanoprobe in enhancing cancer diagnostics, helping medical doctors making treatment decisions more swiftly and effectively.http://www.sciencedirect.com/science/article/pii/S2472630324001110Gold nanoprobeNTR detectionCellular hypoxia monitoringXenograft modelSurface enhanced Raman scatteringBiomedical imaging |
| spellingShingle | Xiaoyue Zhao Ying Zhang Chunyan Zhu Zhihui Yang Xiaoyuan Chu Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosis SLAS Technology Gold nanoprobe NTR detection Cellular hypoxia monitoring Xenograft model Surface enhanced Raman scattering Biomedical imaging |
| title | Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosis |
| title_full | Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosis |
| title_fullStr | Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosis |
| title_full_unstemmed | Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosis |
| title_short | Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosis |
| title_sort | advanced surface enhanced raman scattering nanoprobes for precise detection of nitroreductase in hypoxic tumor cells improving cancer diagnosis |
| topic | Gold nanoprobe NTR detection Cellular hypoxia monitoring Xenograft model Surface enhanced Raman scattering Biomedical imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2472630324001110 |
| work_keys_str_mv | AT xiaoyuezhao advancedsurfaceenhancedramanscatteringnanoprobesforprecisedetectionofnitroreductaseinhypoxictumorcellsimprovingcancerdiagnosis AT yingzhang advancedsurfaceenhancedramanscatteringnanoprobesforprecisedetectionofnitroreductaseinhypoxictumorcellsimprovingcancerdiagnosis AT chunyanzhu advancedsurfaceenhancedramanscatteringnanoprobesforprecisedetectionofnitroreductaseinhypoxictumorcellsimprovingcancerdiagnosis AT zhihuiyang advancedsurfaceenhancedramanscatteringnanoprobesforprecisedetectionofnitroreductaseinhypoxictumorcellsimprovingcancerdiagnosis AT xiaoyuanchu advancedsurfaceenhancedramanscatteringnanoprobesforprecisedetectionofnitroreductaseinhypoxictumorcellsimprovingcancerdiagnosis |