Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids
The aim of the present study was to investigate the interaction between ovarian steroids, interleukins and prostaglandins (PG) in equine epithelial and stromal cells in vitro. In Experiment 1, cells were exposed to IL-1α (10 ng/mL), IL-1β (10 ng/mL) or IL-6 (10 ng/mL) for 24 h and cell proliferation...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/208103 |
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| author | Anna Z. Szóstek Antonio M. Galvão Takuo Hojo Kiyoshi Okuda Dariusz J. Skarzynski |
| author_facet | Anna Z. Szóstek Antonio M. Galvão Takuo Hojo Kiyoshi Okuda Dariusz J. Skarzynski |
| author_sort | Anna Z. Szóstek |
| collection | DOAJ |
| description | The aim of the present study was to investigate the interaction between ovarian steroids, interleukins and prostaglandins (PG) in equine epithelial and stromal cells in vitro. In Experiment 1, cells were exposed to IL-1α (10 ng/mL), IL-1β (10 ng/mL) or IL-6 (10 ng/mL) for 24 h and cell proliferation was determined using MTT. In Experiment 2, cells were exposed to progesterone (P4; 10−7 M); 17-β estradiol (E2; 10−9 M) or P4+E2 for 24 h and later medium was replaced with a fresh one treated with IL-1α, IL-1β or IL-6 (10 ng/mL, each) for 24 h. The oxytocin (OT; 10−7 M) was used as a positive control. In Experiment 3, cells were exposed to P4 (10−7 M), E2 (10−9 M) or P4+E2 for 24 h and the IL receptor mRNAs transcription was determined using Real-time PCR. Prostaglandins concentration was determined using the direct enzyme immunoassay (EIA) method. Our findings reveal a functional linking between ovarian steroids and IL-stimulated PG secretion by equine endometrial cells. This interaction could be one of the mechanisms responsible for endometrial local orchestrating events during the estrous cycle and early pregnancy. |
| format | Article |
| id | doaj-art-03c5497234aa47d88b019ff7d85f5935 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-03c5497234aa47d88b019ff7d85f59352025-02-03T01:01:49ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/208103208103Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian SteroidsAnna Z. Szóstek0Antonio M. Galvão1Takuo Hojo2Kiyoshi Okuda3Dariusz J. Skarzynski4Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, PolandDepartment of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, PolandDepartment of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, PolandLaboratory of Reproductive Endocrinology Graduate School of Natural Science and Technology, Okayama University, 700-8530 Okayama, JapanDepartment of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, PolandThe aim of the present study was to investigate the interaction between ovarian steroids, interleukins and prostaglandins (PG) in equine epithelial and stromal cells in vitro. In Experiment 1, cells were exposed to IL-1α (10 ng/mL), IL-1β (10 ng/mL) or IL-6 (10 ng/mL) for 24 h and cell proliferation was determined using MTT. In Experiment 2, cells were exposed to progesterone (P4; 10−7 M); 17-β estradiol (E2; 10−9 M) or P4+E2 for 24 h and later medium was replaced with a fresh one treated with IL-1α, IL-1β or IL-6 (10 ng/mL, each) for 24 h. The oxytocin (OT; 10−7 M) was used as a positive control. In Experiment 3, cells were exposed to P4 (10−7 M), E2 (10−9 M) or P4+E2 for 24 h and the IL receptor mRNAs transcription was determined using Real-time PCR. Prostaglandins concentration was determined using the direct enzyme immunoassay (EIA) method. Our findings reveal a functional linking between ovarian steroids and IL-stimulated PG secretion by equine endometrial cells. This interaction could be one of the mechanisms responsible for endometrial local orchestrating events during the estrous cycle and early pregnancy.http://dx.doi.org/10.1155/2014/208103 |
| spellingShingle | Anna Z. Szóstek Antonio M. Galvão Takuo Hojo Kiyoshi Okuda Dariusz J. Skarzynski Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids Mediators of Inflammation |
| title | Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids |
| title_full | Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids |
| title_fullStr | Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids |
| title_full_unstemmed | Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids |
| title_short | Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids |
| title_sort | interleukins affect equine endometrial cell function modulatory action of ovarian steroids |
| url | http://dx.doi.org/10.1155/2014/208103 |
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