First Report and Phylogenetic Analysis of Mitochondrial Genomes of <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i>
The mitochondrial genome, highly conserved across species, is crucial for species identification, phylogenetic analysis, and evolutionary research. <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i>, two species with significant forensic value, have been understudi...
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2024-12-01
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| author | Fengqin Yang Jiao Xiao Xiangyan Zhang Yanjie Shang Yadong Guo |
| author_facet | Fengqin Yang Jiao Xiao Xiangyan Zhang Yanjie Shang Yadong Guo |
| author_sort | Fengqin Yang |
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| description | The mitochondrial genome, highly conserved across species, is crucial for species identification, phylogenetic analysis, and evolutionary research. <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i>, two species with significant forensic value, have been understudied in terms of genetic data. In this study, the complete mitochondrial genomes of <i>C. villeneuvi</i> (15,623 bp) and <i>S. genuforceps</i> (15,729 bp) were sequenced and analyzed. All thirteen protein-coding genes (PCGs) exhibited Ka/Ks ratios below one, indicating purifying selection and supporting their utility as barcoding markers. Phylogenetic analysis and genetic distance calculations based on PCGs showed that <i>C. villeneuvi</i> is closely related to <i>Chrysomya rufifacies</i> and <i>Chrysomya albiceps</i>, and <i>S. genuforceps</i> aligns more closely with <i>Sarcophaga kentejana</i> and <i>Sarcophaga schuetzei</i>. This research is the first to provide mitochondrial genome data for <i>C. villeneuvi</i> and <i>S. genuforceps</i>, expanding the genetic resources available for Calliphoridae and Sarcophagidae and offering a foundation for further forensic and evolutionary studies. |
| format | Article |
| id | doaj-art-03c3453ef6874d0fb9d00c9971fa734f |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj-art-03c3453ef6874d0fb9d00c9971fa734f2025-01-24T13:35:38ZengMDPI AGInsects2075-44502024-12-011612610.3390/insects16010026First Report and Phylogenetic Analysis of Mitochondrial Genomes of <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i>Fengqin Yang0Jiao Xiao1Xiangyan Zhang2Yanjie Shang3Yadong Guo4Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaThe mitochondrial genome, highly conserved across species, is crucial for species identification, phylogenetic analysis, and evolutionary research. <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i>, two species with significant forensic value, have been understudied in terms of genetic data. In this study, the complete mitochondrial genomes of <i>C. villeneuvi</i> (15,623 bp) and <i>S. genuforceps</i> (15,729 bp) were sequenced and analyzed. All thirteen protein-coding genes (PCGs) exhibited Ka/Ks ratios below one, indicating purifying selection and supporting their utility as barcoding markers. Phylogenetic analysis and genetic distance calculations based on PCGs showed that <i>C. villeneuvi</i> is closely related to <i>Chrysomya rufifacies</i> and <i>Chrysomya albiceps</i>, and <i>S. genuforceps</i> aligns more closely with <i>Sarcophaga kentejana</i> and <i>Sarcophaga schuetzei</i>. This research is the first to provide mitochondrial genome data for <i>C. villeneuvi</i> and <i>S. genuforceps</i>, expanding the genetic resources available for Calliphoridae and Sarcophagidae and offering a foundation for further forensic and evolutionary studies.https://www.mdpi.com/2075-4450/16/1/26mitochondrial genomics<i>Chrysomya villeneuvi</i><i>Sarcophaga genuforceps</i>phylogenetic analysis |
| spellingShingle | Fengqin Yang Jiao Xiao Xiangyan Zhang Yanjie Shang Yadong Guo First Report and Phylogenetic Analysis of Mitochondrial Genomes of <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i> Insects mitochondrial genomics <i>Chrysomya villeneuvi</i> <i>Sarcophaga genuforceps</i> phylogenetic analysis |
| title | First Report and Phylogenetic Analysis of Mitochondrial Genomes of <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i> |
| title_full | First Report and Phylogenetic Analysis of Mitochondrial Genomes of <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i> |
| title_fullStr | First Report and Phylogenetic Analysis of Mitochondrial Genomes of <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i> |
| title_full_unstemmed | First Report and Phylogenetic Analysis of Mitochondrial Genomes of <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i> |
| title_short | First Report and Phylogenetic Analysis of Mitochondrial Genomes of <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i> |
| title_sort | first report and phylogenetic analysis of mitochondrial genomes of i chrysomya villeneuvi i and i sarcophaga genuforceps i |
| topic | mitochondrial genomics <i>Chrysomya villeneuvi</i> <i>Sarcophaga genuforceps</i> phylogenetic analysis |
| url | https://www.mdpi.com/2075-4450/16/1/26 |
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