First Report and Phylogenetic Analysis of Mitochondrial Genomes of <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i>
The mitochondrial genome, highly conserved across species, is crucial for species identification, phylogenetic analysis, and evolutionary research. <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i>, two species with significant forensic value, have been understudi...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/16/1/26 |
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| Summary: | The mitochondrial genome, highly conserved across species, is crucial for species identification, phylogenetic analysis, and evolutionary research. <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i>, two species with significant forensic value, have been understudied in terms of genetic data. In this study, the complete mitochondrial genomes of <i>C. villeneuvi</i> (15,623 bp) and <i>S. genuforceps</i> (15,729 bp) were sequenced and analyzed. All thirteen protein-coding genes (PCGs) exhibited Ka/Ks ratios below one, indicating purifying selection and supporting their utility as barcoding markers. Phylogenetic analysis and genetic distance calculations based on PCGs showed that <i>C. villeneuvi</i> is closely related to <i>Chrysomya rufifacies</i> and <i>Chrysomya albiceps</i>, and <i>S. genuforceps</i> aligns more closely with <i>Sarcophaga kentejana</i> and <i>Sarcophaga schuetzei</i>. This research is the first to provide mitochondrial genome data for <i>C. villeneuvi</i> and <i>S. genuforceps</i>, expanding the genetic resources available for Calliphoridae and Sarcophagidae and offering a foundation for further forensic and evolutionary studies. |
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| ISSN: | 2075-4450 |