Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems
The aim of this study is to present a new method for determining the root-derived extracellular acid phosphomonoesterase (EAPM) activity fraction within the total EAPM activity of soil. EAPM activity was determined for roots, organic and mineral soil. Samples were collected using paired PVC cylinder...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/250805 |
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| author | Klement Rejsek Valerie Vranova Pavel Formanek |
| author_facet | Klement Rejsek Valerie Vranova Pavel Formanek |
| author_sort | Klement Rejsek |
| collection | DOAJ |
| description | The aim of this study is to present a new method for determining the root-derived extracellular acid phosphomonoesterase (EAPM) activity fraction within the total EAPM activity of soil. EAPM activity was determined for roots, organic and mineral soil. Samples were collected using paired PVC cylinders, inserted to a depth of 15 cm, within seven selected forest stands. Root-derived EAPM formed between 4 and18% of the total EAPM activity of soil from forests of differing maturity. A new approach, presented in this work, enables separation of root-derived EAPM activity from total soil EAPM. Separation of root-derived EAPM from soil provides a better understanding of its role in P-cycling in terrestrial ecosystems. The method presented in this work is a first step towards the separation of root- and microbe-derived EAPM in soils, which are thought to possess different kinetic properties and different sensitivity to environmental change. |
| format | Article |
| id | doaj-art-03be9990245a496fb227319f790b1c33 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-03be9990245a496fb227319f790b1c332025-02-03T01:26:21ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/250805250805Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest EcosystemsKlement Rejsek0Valerie Vranova1Pavel Formanek2Department of Geology and Soil Science, Mendel University in Brno, Zemedelska 3, 613 00, Brno, Czech RepublicDepartment of Geology and Soil Science, Mendel University in Brno, Zemedelska 3, 613 00, Brno, Czech RepublicDepartment of Geology and Soil Science, Mendel University in Brno, Zemedelska 3, 613 00, Brno, Czech RepublicThe aim of this study is to present a new method for determining the root-derived extracellular acid phosphomonoesterase (EAPM) activity fraction within the total EAPM activity of soil. EAPM activity was determined for roots, organic and mineral soil. Samples were collected using paired PVC cylinders, inserted to a depth of 15 cm, within seven selected forest stands. Root-derived EAPM formed between 4 and18% of the total EAPM activity of soil from forests of differing maturity. A new approach, presented in this work, enables separation of root-derived EAPM activity from total soil EAPM. Separation of root-derived EAPM from soil provides a better understanding of its role in P-cycling in terrestrial ecosystems. The method presented in this work is a first step towards the separation of root- and microbe-derived EAPM in soils, which are thought to possess different kinetic properties and different sensitivity to environmental change.http://dx.doi.org/10.1100/2012/250805 |
| spellingShingle | Klement Rejsek Valerie Vranova Pavel Formanek Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems The Scientific World Journal |
| title | Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems |
| title_full | Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems |
| title_fullStr | Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems |
| title_full_unstemmed | Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems |
| title_short | Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems |
| title_sort | determination of the proportion of total soil extracellular acid phosphomonoesterase e c 3 1 3 2 activity represented by roots in the soil of different forest ecosystems |
| url | http://dx.doi.org/10.1100/2012/250805 |
| work_keys_str_mv | AT klementrejsek determinationoftheproportionoftotalsoilextracellularacidphosphomonoesteraseec3132activityrepresentedbyrootsinthesoilofdifferentforestecosystems AT valerievranova determinationoftheproportionoftotalsoilextracellularacidphosphomonoesteraseec3132activityrepresentedbyrootsinthesoilofdifferentforestecosystems AT pavelformanek determinationoftheproportionoftotalsoilextracellularacidphosphomonoesteraseec3132activityrepresentedbyrootsinthesoilofdifferentforestecosystems |