A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker
In this study, a new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists—ondansetron, azasetron, ramosetron, granisetron, and tropisetron—either in infusion samples or in injection dosage form was first established based on high-performance liquid ch...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/5533646 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561332142997504 |
|---|---|
| author | Fuchao Chen Baoxia Fang Peng Li Sicen Wang |
| author_facet | Fuchao Chen Baoxia Fang Peng Li Sicen Wang |
| author_sort | Fuchao Chen |
| collection | DOAJ |
| description | In this study, a new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists—ondansetron, azasetron, ramosetron, granisetron, and tropisetron—either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. The quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. The quantitative analysis of the 5-HT3 receptor antagonists was calculated and investigated based on the relative correction factors. Chromatographic separation was achieved using a C18 column (150 mm × 4.6 mm, 5.0 μm), and the mobile phase was composed of acetonitrile-0.05 mol·L−1 potassium dihydrogen phosphate (pH 4.0) (25 : 75) at a flow rate of 1.0 mL·min−1 and detection wavelengths of 307 nm (ondansetron, azasetron, ramosetron), 302 nm (granisetron), and 285 nm (tropisetron). In addition, the accuracy of the quantitative analysis of multicomponents by a single marker method was compared with an external standard method, and no significant difference was observed between the two methods. The established method is rapid, is easy, and does not require many reference substances, and it can been successfully applied as part of the quality control of the five 5-HT3 receptor antagonists in their injection dosage form and infusion sample drugs in hospitals. |
| format | Article |
| id | doaj-art-038e29405a7c443e86e83f3774a663fd |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-038e29405a7c443e86e83f3774a663fd2025-02-03T01:25:11ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792021-01-01202110.1155/2021/55336465533646A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single MarkerFuchao Chen0Baoxia Fang1Peng Li2Sicen Wang3Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, ChinaSinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, ChinaSinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, ChinaSinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, ChinaIn this study, a new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists—ondansetron, azasetron, ramosetron, granisetron, and tropisetron—either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. The quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. The quantitative analysis of the 5-HT3 receptor antagonists was calculated and investigated based on the relative correction factors. Chromatographic separation was achieved using a C18 column (150 mm × 4.6 mm, 5.0 μm), and the mobile phase was composed of acetonitrile-0.05 mol·L−1 potassium dihydrogen phosphate (pH 4.0) (25 : 75) at a flow rate of 1.0 mL·min−1 and detection wavelengths of 307 nm (ondansetron, azasetron, ramosetron), 302 nm (granisetron), and 285 nm (tropisetron). In addition, the accuracy of the quantitative analysis of multicomponents by a single marker method was compared with an external standard method, and no significant difference was observed between the two methods. The established method is rapid, is easy, and does not require many reference substances, and it can been successfully applied as part of the quality control of the five 5-HT3 receptor antagonists in their injection dosage form and infusion sample drugs in hospitals.http://dx.doi.org/10.1155/2021/5533646 |
| spellingShingle | Fuchao Chen Baoxia Fang Peng Li Sicen Wang A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker International Journal of Analytical Chemistry |
| title | A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker |
| title_full | A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker |
| title_fullStr | A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker |
| title_full_unstemmed | A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker |
| title_short | A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker |
| title_sort | fast and validated hplc method for the simultaneous analysis of five 5 ht3 receptor antagonists via the quantitative analysis of multicomponents by a single marker |
| url | http://dx.doi.org/10.1155/2021/5533646 |
| work_keys_str_mv | AT fuchaochen afastandvalidatedhplcmethodforthesimultaneousanalysisoffive5ht3receptorantagonistsviathequantitativeanalysisofmulticomponentsbyasinglemarker AT baoxiafang afastandvalidatedhplcmethodforthesimultaneousanalysisoffive5ht3receptorantagonistsviathequantitativeanalysisofmulticomponentsbyasinglemarker AT pengli afastandvalidatedhplcmethodforthesimultaneousanalysisoffive5ht3receptorantagonistsviathequantitativeanalysisofmulticomponentsbyasinglemarker AT sicenwang afastandvalidatedhplcmethodforthesimultaneousanalysisoffive5ht3receptorantagonistsviathequantitativeanalysisofmulticomponentsbyasinglemarker AT fuchaochen fastandvalidatedhplcmethodforthesimultaneousanalysisoffive5ht3receptorantagonistsviathequantitativeanalysisofmulticomponentsbyasinglemarker AT baoxiafang fastandvalidatedhplcmethodforthesimultaneousanalysisoffive5ht3receptorantagonistsviathequantitativeanalysisofmulticomponentsbyasinglemarker AT pengli fastandvalidatedhplcmethodforthesimultaneousanalysisoffive5ht3receptorantagonistsviathequantitativeanalysisofmulticomponentsbyasinglemarker AT sicenwang fastandvalidatedhplcmethodforthesimultaneousanalysisoffive5ht3receptorantagonistsviathequantitativeanalysisofmulticomponentsbyasinglemarker |