Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose
While TOFA (acetyl CoA carboxylase inhibitor) and C75 (fatty acid synthase inhibitor) prevent lipid accumulation by inhibiting fatty acid synthesis, the mechanism of action is not simply accounted for by inhibition of the enzymes alone. Liver fatty acid binding protein (L-FABP), a medi...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2013/865604 |
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| author | Huan Huang Avery L. McIntosh Gregory G. Martin Anca D. Petrescu Kerstin K. Landrock Danilo Landrock Ann B. Kier Friedhelm Schroeder |
| author_facet | Huan Huang Avery L. McIntosh Gregory G. Martin Anca D. Petrescu Kerstin K. Landrock Danilo Landrock Ann B. Kier Friedhelm Schroeder |
| author_sort | Huan Huang |
| collection | DOAJ |
| description | While TOFA (acetyl CoA carboxylase inhibitor) and C75 (fatty acid synthase inhibitor) prevent lipid accumulation by inhibiting fatty acid synthesis, the mechanism of action is not simply accounted for by inhibition of the enzymes alone.
Liver fatty acid binding protein (L-FABP), a mediator of long chain fatty acid signaling to peroxisome
proliferator-activated receptor-α (PPARα) in the nucleus, was found to bind
TOFA and its activated CoA thioester, TOFyl-CoA, with high affinity while binding C75 and C75-CoA
with lower affinity. Binding of TOFA and C75-CoA significantly altered L-FABP secondary structure. High (20 mM) but not physiological
(6 mM) glucose conferred on both TOFA and C75 the ability to induce PPARα transcription of the fatty
acid β-oxidative enzymes CPT1A, CPT2, and ACOX1 in cultured primary hepatocytes from wild-type (WT) mice.
However, L-FABP gene ablation abolished the effects of TOFA and C75 in the context of high glucose. These effects were not associated
with an increased cellular level of unesterified fatty acids but rather by increased intracellular glucose. These findings suggested that L-FABP may function as an intracellular fatty acid synthesis inhibitor binding protein
facilitating TOFA and C75-mediated induction of PPARα in the context of high glucose at levels similar to those in uncontrolled diabetes. |
| format | Article |
| id | doaj-art-03843b8ce0c74258acc30041501f3f1c |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-03843b8ce0c74258acc30041501f3f1c2025-02-03T05:46:28ZengWileyPPAR Research1687-47571687-47652013-01-01201310.1155/2013/865604865604Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and GlucoseHuan Huang0Avery L. McIntosh1Gregory G. Martin2Anca D. Petrescu3Kerstin K. Landrock4Danilo Landrock5Ann B. Kier6Friedhelm Schroeder7Department of Physiology and Pharmacology, Texas A&M University, TAMU 4466, College Station, TX 77843-4466, USADepartment of Physiology and Pharmacology, Texas A&M University, TAMU 4466, College Station, TX 77843-4466, USADepartment of Physiology and Pharmacology, Texas A&M University, TAMU 4466, College Station, TX 77843-4466, USADepartment of Physiology and Pharmacology, Texas A&M University, TAMU 4466, College Station, TX 77843-4466, USADepartment of Physiology and Pharmacology, Texas A&M University, TAMU 4466, College Station, TX 77843-4466, USADepartment of Pathobiology, Texas A&M University, TAMU 4467, College Station, TX 77843-4467, USADepartment of Pathobiology, Texas A&M University, TAMU 4467, College Station, TX 77843-4467, USADepartment of Physiology and Pharmacology, Texas A&M University, TAMU 4466, College Station, TX 77843-4466, USAWhile TOFA (acetyl CoA carboxylase inhibitor) and C75 (fatty acid synthase inhibitor) prevent lipid accumulation by inhibiting fatty acid synthesis, the mechanism of action is not simply accounted for by inhibition of the enzymes alone. Liver fatty acid binding protein (L-FABP), a mediator of long chain fatty acid signaling to peroxisome proliferator-activated receptor-α (PPARα) in the nucleus, was found to bind TOFA and its activated CoA thioester, TOFyl-CoA, with high affinity while binding C75 and C75-CoA with lower affinity. Binding of TOFA and C75-CoA significantly altered L-FABP secondary structure. High (20 mM) but not physiological (6 mM) glucose conferred on both TOFA and C75 the ability to induce PPARα transcription of the fatty acid β-oxidative enzymes CPT1A, CPT2, and ACOX1 in cultured primary hepatocytes from wild-type (WT) mice. However, L-FABP gene ablation abolished the effects of TOFA and C75 in the context of high glucose. These effects were not associated with an increased cellular level of unesterified fatty acids but rather by increased intracellular glucose. These findings suggested that L-FABP may function as an intracellular fatty acid synthesis inhibitor binding protein facilitating TOFA and C75-mediated induction of PPARα in the context of high glucose at levels similar to those in uncontrolled diabetes.http://dx.doi.org/10.1155/2013/865604 |
| spellingShingle | Huan Huang Avery L. McIntosh Gregory G. Martin Anca D. Petrescu Kerstin K. Landrock Danilo Landrock Ann B. Kier Friedhelm Schroeder Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose PPAR Research |
| title | Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose |
| title_full | Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose |
| title_fullStr | Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose |
| title_full_unstemmed | Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose |
| title_short | Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose |
| title_sort | inhibitors of fatty acid synthesis induce pparα regulated fatty acid β oxidative genes synergistic roles of l fabp and glucose |
| url | http://dx.doi.org/10.1155/2013/865604 |
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