A transcriptomic landscape analysis of human necrotizing enterocolitis: Important roles of immune infiltration
Abstract Necrotizing enterocolitis (NEC) is one of the most common and destructive diseases in neonates and an unpredictable surgical emergency. However, the molecular pathological mechanism of NEC is still not well understood. This study was designed to provide a molecular basis for the pathogenesi...
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Pediatric Discovery |
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| Online Access: | https://doi.org/10.1002/pdi3.1 |
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| author | Zhuojun Xie Quan Kang Yulu Shi Junbao Du Hao Jiang |
| author_facet | Zhuojun Xie Quan Kang Yulu Shi Junbao Du Hao Jiang |
| author_sort | Zhuojun Xie |
| collection | DOAJ |
| description | Abstract Necrotizing enterocolitis (NEC) is one of the most common and destructive diseases in neonates and an unpredictable surgical emergency. However, the molecular pathological mechanism of NEC is still not well understood. This study was designed to provide a molecular basis for the pathogenesis of human NEC through bioinformatics analysis and immune infiltration. For RNA‐Seq, DEseq2 algorithm was used to identify differentially expressed genes (DEGs) and to perform functional enrichment analysis. Immune infiltration was analyzed by CIBERSORT algorithm. A total of 34,712 genes were detected and 7463 DEGs were identified in this study. Gene Ontology analysis revealed that DEGs were mainly involved in CCR1 chemokine receptor binding, transporter activity, growth factor binding, etc. KEGG pathway analysis showed that the DEGs were significantly enriched in the toll‐like receptor signaling pathway, Th17 cell differentiation, and cytokine–cytokine receptor interaction. The immune infiltration profiles varied significantly between NEC, NEC self‐control, and normal intestinal tissues. Finally, the expression levels of 21 DEGs were verified by reverse transcription quantitative real‐time PCR. Our findings may provide new insights into the development of NEC. |
| format | Article |
| id | doaj-art-0376b15dbe5646f2a63b9236c93ec1c5 |
| institution | OA Journals |
| issn | 2835-558X 2835-5598 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pediatric Discovery |
| spelling | doaj-art-0376b15dbe5646f2a63b9236c93ec1c52025-08-20T02:16:56ZengWileyPediatric Discovery2835-558X2835-55982023-06-0111n/an/a10.1002/pdi3.1A transcriptomic landscape analysis of human necrotizing enterocolitis: Important roles of immune infiltrationZhuojun Xie0Quan Kang1Yulu Shi2Junbao Du3Hao Jiang4Stem Cell Biology and Therapy Laboratory The Children's Hospital of Chongqing Medical University National Clinical Research Center for Child Health and Disorders Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Pediatrics Chongqing ChinaDepartment of General Surgery The Children's Hospital of Chongqing Medical University National Clinical Research Center for Child Health and Disorders Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Pediatrics Chongqing ChinaStem Cell Biology and Therapy Laboratory The Children's Hospital of Chongqing Medical University National Clinical Research Center for Child Health and Disorders Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Pediatrics Chongqing ChinaStem Cell Biology and Therapy Laboratory The Children's Hospital of Chongqing Medical University National Clinical Research Center for Child Health and Disorders Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Pediatrics Chongqing ChinaPediatric Ward 1 Taihe Hospital Shiyan City Hubei ChinaAbstract Necrotizing enterocolitis (NEC) is one of the most common and destructive diseases in neonates and an unpredictable surgical emergency. However, the molecular pathological mechanism of NEC is still not well understood. This study was designed to provide a molecular basis for the pathogenesis of human NEC through bioinformatics analysis and immune infiltration. For RNA‐Seq, DEseq2 algorithm was used to identify differentially expressed genes (DEGs) and to perform functional enrichment analysis. Immune infiltration was analyzed by CIBERSORT algorithm. A total of 34,712 genes were detected and 7463 DEGs were identified in this study. Gene Ontology analysis revealed that DEGs were mainly involved in CCR1 chemokine receptor binding, transporter activity, growth factor binding, etc. KEGG pathway analysis showed that the DEGs were significantly enriched in the toll‐like receptor signaling pathway, Th17 cell differentiation, and cytokine–cytokine receptor interaction. The immune infiltration profiles varied significantly between NEC, NEC self‐control, and normal intestinal tissues. Finally, the expression levels of 21 DEGs were verified by reverse transcription quantitative real‐time PCR. Our findings may provide new insights into the development of NEC.https://doi.org/10.1002/pdi3.1gene expressionimmune infiltrationnecrotizing enterocolitisRNA‐seq |
| spellingShingle | Zhuojun Xie Quan Kang Yulu Shi Junbao Du Hao Jiang A transcriptomic landscape analysis of human necrotizing enterocolitis: Important roles of immune infiltration Pediatric Discovery gene expression immune infiltration necrotizing enterocolitis RNA‐seq |
| title | A transcriptomic landscape analysis of human necrotizing enterocolitis: Important roles of immune infiltration |
| title_full | A transcriptomic landscape analysis of human necrotizing enterocolitis: Important roles of immune infiltration |
| title_fullStr | A transcriptomic landscape analysis of human necrotizing enterocolitis: Important roles of immune infiltration |
| title_full_unstemmed | A transcriptomic landscape analysis of human necrotizing enterocolitis: Important roles of immune infiltration |
| title_short | A transcriptomic landscape analysis of human necrotizing enterocolitis: Important roles of immune infiltration |
| title_sort | transcriptomic landscape analysis of human necrotizing enterocolitis important roles of immune infiltration |
| topic | gene expression immune infiltration necrotizing enterocolitis RNA‐seq |
| url | https://doi.org/10.1002/pdi3.1 |
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