Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity In Enzymatic Dehairing Process
In the present study, Aspergillus parasiticus TEM obtained from our culture collection produced appreciable quantities (2955.739.4 U/gds) of extracellular protease, but negligible quantities of keratinase, when grown on wheat bran moistened to 75% with Czapex Dox solution containing 1% NaNO3 by mea...
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| Format: | Article |
| Language: | English |
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Tokat Gaziosmanpasa University
2015-05-01
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| Series: | Journal of New Results in Science |
| Online Access: | https://dergipark.org.tr/en/download/article-file/105135 |
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| author | Behzat Oral Bıtlıslı Bilge Hilal Cadirci Huseyin ATA Karavana Ihsan Yasa |
| author_facet | Behzat Oral Bıtlıslı Bilge Hilal Cadirci Huseyin ATA Karavana Ihsan Yasa |
| author_sort | Behzat Oral Bıtlıslı |
| collection | DOAJ |
| description | In the present study, Aspergillus parasiticus TEM obtained from our culture collection produced appreciable quantities (2955.739.4 U/gds) of extracellular protease, but negligible quantities of keratinase, when grown on wheat bran moistened to 75% with Czapex Dox solution containing 1% NaNO3 by means of solid-state fermentation (SSF). After incubation at 27C for 3 days, A. parasiticus TEM reached to a maximum protease activity. The optimum pH of purified enzyme was around pH 8.0 at 30C, but substantial activity was recorded at the pH 7.0-11.0 and it is stable pH values ranging from 7.0-9.0 for 8 hours. Temperature optimum of the enzyme was found to be at 50 oC without Ca++ and to be highly stable (90%) at 30oC for 8 hours. When the protease from A. parasiticus TEM was used in dehairing process of cattle hide, the mixture of 100% crude enzyme, 1.5% lime and 1% sodium sulphide based on hide weight was completely dehaired hides at 30oC for 6 hours, but crude enzyme 200% alone carried out this process for 8 hours |
| format | Article |
| id | doaj-art-035db37bc5c943ae8f6ce0a4f5dc71cb |
| institution | DOAJ |
| issn | 1304-7981 |
| language | English |
| publishDate | 2015-05-01 |
| publisher | Tokat Gaziosmanpasa University |
| record_format | Article |
| series | Journal of New Results in Science |
| spelling | doaj-art-035db37bc5c943ae8f6ce0a4f5dc71cb2025-08-20T02:41:19ZengTokat Gaziosmanpasa UniversityJournal of New Results in Science1304-79812015-05-01487283142Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity In Enzymatic Dehairing ProcessBehzat Oral BıtlıslıBilge Hilal CadirciHuseyin ATA KaravanaIhsan YasaIn the present study, Aspergillus parasiticus TEM obtained from our culture collection produced appreciable quantities (2955.739.4 U/gds) of extracellular protease, but negligible quantities of keratinase, when grown on wheat bran moistened to 75% with Czapex Dox solution containing 1% NaNO3 by means of solid-state fermentation (SSF). After incubation at 27C for 3 days, A. parasiticus TEM reached to a maximum protease activity. The optimum pH of purified enzyme was around pH 8.0 at 30C, but substantial activity was recorded at the pH 7.0-11.0 and it is stable pH values ranging from 7.0-9.0 for 8 hours. Temperature optimum of the enzyme was found to be at 50 oC without Ca++ and to be highly stable (90%) at 30oC for 8 hours. When the protease from A. parasiticus TEM was used in dehairing process of cattle hide, the mixture of 100% crude enzyme, 1.5% lime and 1% sodium sulphide based on hide weight was completely dehaired hides at 30oC for 6 hours, but crude enzyme 200% alone carried out this process for 8 hourshttps://dergipark.org.tr/en/download/article-file/105135 |
| spellingShingle | Behzat Oral Bıtlıslı Bilge Hilal Cadirci Huseyin ATA Karavana Ihsan Yasa Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity In Enzymatic Dehairing Process Journal of New Results in Science |
| title | Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity In Enzymatic Dehairing Process |
| title_full | Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity In Enzymatic Dehairing Process |
| title_fullStr | Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity In Enzymatic Dehairing Process |
| title_full_unstemmed | Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity In Enzymatic Dehairing Process |
| title_short | Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity In Enzymatic Dehairing Process |
| title_sort | thermostable serine protease by aspergillus parasiticus tem have promising activity in enzymatic dehairing process |
| url | https://dergipark.org.tr/en/download/article-file/105135 |
| work_keys_str_mv | AT behzatoralbıtlıslı thermostableserineproteasebyaspergillusparasiticustemhavepromisingactivityinenzymaticdehairingprocess AT bilgehilalcadirci thermostableserineproteasebyaspergillusparasiticustemhavepromisingactivityinenzymaticdehairingprocess AT huseyinatakaravana thermostableserineproteasebyaspergillusparasiticustemhavepromisingactivityinenzymaticdehairingprocess AT ihsanyasa thermostableserineproteasebyaspergillusparasiticustemhavepromisingactivityinenzymaticdehairingprocess |