Reorganization of Extracellular Matrix in Placentas from Women with Asymptomatic Chagas Disease: Mechanism of Parasite Invasion or Local Placental Defense?
Chagas disease, produced by the protozoan Trypanosoma cruzi (T. cruzi), is one of the most frequent endemic diseases in Latin America. In spite the fact that in the past few years T. cruzi congenital transmission has become of epidemiological importance, studies about this mechanism of infection ar...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/758357 |
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| author | Juan Duaso Erika Yanez Christian Castillo Norbel Galanti Gonzalo Cabrera Gabriela Corral Juan Diego Maya Inés Zulantay Werner Apt Ulrike Kemmerling |
| author_facet | Juan Duaso Erika Yanez Christian Castillo Norbel Galanti Gonzalo Cabrera Gabriela Corral Juan Diego Maya Inés Zulantay Werner Apt Ulrike Kemmerling |
| author_sort | Juan Duaso |
| collection | DOAJ |
| description | Chagas disease, produced by the protozoan Trypanosoma cruzi (T. cruzi), is one of the most frequent endemic diseases in Latin America. In spite the fact that in the past few years T. cruzi congenital transmission has become of epidemiological importance, studies about this mechanism of infection are scarce. In order to explore some morphological aspects of this infection in the placenta, we analyzed placentas from T. cruzi-infected mothers by immunohistochemical and histochemical methods. Infection in mothers, newborns, and placentas was confirmed by PCR and by immunofluorescence in the placenta. T. cruzi-infected placentas present destruction of the syncytiotrophoblast and villous stroma, selective disorganization of the basal lamina, and disorganization of collagen I in villous stroma. Our results suggest that the parasite induces reorganization of this tissue component and in this way may regulate both inflammatory and immune responses in the host. Changes in the ECM of placental tissues, together with the immunological status of mother and fetus, and parasite load may determine the probability of congenital transmission of T. cruzi. |
| format | Article |
| id | doaj-art-035a471975304f4dad13de1952f0e7ce |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-035a471975304f4dad13de1952f0e7ce2025-02-03T01:28:00ZengWileyJournal of Tropical Medicine1687-96861687-96942012-01-01201210.1155/2012/758357758357Reorganization of Extracellular Matrix in Placentas from Women with Asymptomatic Chagas Disease: Mechanism of Parasite Invasion or Local Placental Defense?Juan Duaso0Erika Yanez1Christian Castillo2Norbel Galanti3Gonzalo Cabrera4Gabriela Corral5Juan Diego Maya6Inés Zulantay7Werner Apt8Ulrike Kemmerling9Programa de Anatomía y Biología del Desarrollo, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia, Región Metropolitana, 1027 Santiago de Chile, ChilePrograma de Anatomía y Biología del Desarrollo, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia, Región Metropolitana, 1027 Santiago de Chile, ChilePrograma de Anatomía y Biología del Desarrollo, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia, Región Metropolitana, 1027 Santiago de Chile, ChilePrograma de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia, Región Metropolitana, 1027 Santiago de Chile, ChilePrograma de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia, Región Metropolitana, 1027 Santiago de Chile, ChileServicio de Obstetricia y Ginecología, Hospital de Illapel, Independencia, IV Región, 0512 Illapel, ChilePrograma de Farmacología Molecular y Clínica, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia, Región Metropolitana, 1027 Santiago de Chile, ChilePrograma de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia, Región Metropolitana, 1027 Santiago de Chile, ChilePrograma de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia, Región Metropolitana, 1027 Santiago de Chile, ChilePrograma de Anatomía y Biología del Desarrollo, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia, Región Metropolitana, 1027 Santiago de Chile, ChileChagas disease, produced by the protozoan Trypanosoma cruzi (T. cruzi), is one of the most frequent endemic diseases in Latin America. In spite the fact that in the past few years T. cruzi congenital transmission has become of epidemiological importance, studies about this mechanism of infection are scarce. In order to explore some morphological aspects of this infection in the placenta, we analyzed placentas from T. cruzi-infected mothers by immunohistochemical and histochemical methods. Infection in mothers, newborns, and placentas was confirmed by PCR and by immunofluorescence in the placenta. T. cruzi-infected placentas present destruction of the syncytiotrophoblast and villous stroma, selective disorganization of the basal lamina, and disorganization of collagen I in villous stroma. Our results suggest that the parasite induces reorganization of this tissue component and in this way may regulate both inflammatory and immune responses in the host. Changes in the ECM of placental tissues, together with the immunological status of mother and fetus, and parasite load may determine the probability of congenital transmission of T. cruzi.http://dx.doi.org/10.1155/2012/758357 |
| spellingShingle | Juan Duaso Erika Yanez Christian Castillo Norbel Galanti Gonzalo Cabrera Gabriela Corral Juan Diego Maya Inés Zulantay Werner Apt Ulrike Kemmerling Reorganization of Extracellular Matrix in Placentas from Women with Asymptomatic Chagas Disease: Mechanism of Parasite Invasion or Local Placental Defense? Journal of Tropical Medicine |
| title | Reorganization of Extracellular Matrix in Placentas from Women with Asymptomatic Chagas Disease: Mechanism of Parasite Invasion or Local Placental Defense? |
| title_full | Reorganization of Extracellular Matrix in Placentas from Women with Asymptomatic Chagas Disease: Mechanism of Parasite Invasion or Local Placental Defense? |
| title_fullStr | Reorganization of Extracellular Matrix in Placentas from Women with Asymptomatic Chagas Disease: Mechanism of Parasite Invasion or Local Placental Defense? |
| title_full_unstemmed | Reorganization of Extracellular Matrix in Placentas from Women with Asymptomatic Chagas Disease: Mechanism of Parasite Invasion or Local Placental Defense? |
| title_short | Reorganization of Extracellular Matrix in Placentas from Women with Asymptomatic Chagas Disease: Mechanism of Parasite Invasion or Local Placental Defense? |
| title_sort | reorganization of extracellular matrix in placentas from women with asymptomatic chagas disease mechanism of parasite invasion or local placental defense |
| url | http://dx.doi.org/10.1155/2012/758357 |
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