BSA-Assisted Synthesis of Au Nanoclusters/MnO<sub>2</sub> Nanosheets for Fluorescence “Switch-On” Detection of Alkaline Phosphatase
A fluorescence probe for “switch-on” detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO<sub>2</sub> nanosheets (Au NCs-MnO<sub>2</sub> NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot a...
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MDPI AG
2025-01-01
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/49 |
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| author | Yijiong Xue Chengqi Bao Hui Liu Fanghui Ma Minghui Yang Xiaoqing Li |
| author_facet | Yijiong Xue Chengqi Bao Hui Liu Fanghui Ma Minghui Yang Xiaoqing Li |
| author_sort | Yijiong Xue |
| collection | DOAJ |
| description | A fluorescence probe for “switch-on” detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO<sub>2</sub> nanosheets (Au NCs-MnO<sub>2</sub> NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot approach. In the sensing system, MnO<sub>2</sub> NSs function as both energy acceptors and target identifiers, effectively quenches the fluorescence of Au NCs via fluorescence resonance energy transfer (FRET). The presence of ALP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), reducing MnO<sub>2</sub> NSs to Mn<sup>2+</sup> and facilitate the fluorescence recovery of Au NCs. The fluorescence assay offers the advantages of facile preparation, cost-effectiveness, good specificity, and high sensitivity. Moreover, the assay exhibits a broad linear range (0.005 U/mL to 8 U/mL) for ALP detection with a remarkable limit of detection of 0.0015 U/mL. Notably, this assay demonstrates promising applicability for detection ALP in human serum samples, thereby providing valuable potential for clinical applications. |
| format | Article |
| id | doaj-art-03318f7413354c818156c37bfef68c9b |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj-art-03318f7413354c818156c37bfef68c9b2025-01-24T13:25:33ZengMDPI AGBiosensors2079-63742025-01-011514910.3390/bios15010049BSA-Assisted Synthesis of Au Nanoclusters/MnO<sub>2</sub> Nanosheets for Fluorescence “Switch-On” Detection of Alkaline PhosphataseYijiong Xue0Chengqi Bao1Hui Liu2Fanghui Ma3Minghui Yang4Xiaoqing Li5Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaHunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaHunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaHunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaHunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaFurong Labratory, Changsha 410083, ChinaA fluorescence probe for “switch-on” detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO<sub>2</sub> nanosheets (Au NCs-MnO<sub>2</sub> NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot approach. In the sensing system, MnO<sub>2</sub> NSs function as both energy acceptors and target identifiers, effectively quenches the fluorescence of Au NCs via fluorescence resonance energy transfer (FRET). The presence of ALP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), reducing MnO<sub>2</sub> NSs to Mn<sup>2+</sup> and facilitate the fluorescence recovery of Au NCs. The fluorescence assay offers the advantages of facile preparation, cost-effectiveness, good specificity, and high sensitivity. Moreover, the assay exhibits a broad linear range (0.005 U/mL to 8 U/mL) for ALP detection with a remarkable limit of detection of 0.0015 U/mL. Notably, this assay demonstrates promising applicability for detection ALP in human serum samples, thereby providing valuable potential for clinical applications.https://www.mdpi.com/2079-6374/15/1/49Au nanoclustersMnO<sub>2</sub> nanosheetsalkaline phosphatasefluorescence resonance energy transfer |
| spellingShingle | Yijiong Xue Chengqi Bao Hui Liu Fanghui Ma Minghui Yang Xiaoqing Li BSA-Assisted Synthesis of Au Nanoclusters/MnO<sub>2</sub> Nanosheets for Fluorescence “Switch-On” Detection of Alkaline Phosphatase Biosensors Au nanoclusters MnO<sub>2</sub> nanosheets alkaline phosphatase fluorescence resonance energy transfer |
| title | BSA-Assisted Synthesis of Au Nanoclusters/MnO<sub>2</sub> Nanosheets for Fluorescence “Switch-On” Detection of Alkaline Phosphatase |
| title_full | BSA-Assisted Synthesis of Au Nanoclusters/MnO<sub>2</sub> Nanosheets for Fluorescence “Switch-On” Detection of Alkaline Phosphatase |
| title_fullStr | BSA-Assisted Synthesis of Au Nanoclusters/MnO<sub>2</sub> Nanosheets for Fluorescence “Switch-On” Detection of Alkaline Phosphatase |
| title_full_unstemmed | BSA-Assisted Synthesis of Au Nanoclusters/MnO<sub>2</sub> Nanosheets for Fluorescence “Switch-On” Detection of Alkaline Phosphatase |
| title_short | BSA-Assisted Synthesis of Au Nanoclusters/MnO<sub>2</sub> Nanosheets for Fluorescence “Switch-On” Detection of Alkaline Phosphatase |
| title_sort | bsa assisted synthesis of au nanoclusters mno sub 2 sub nanosheets for fluorescence switch on detection of alkaline phosphatase |
| topic | Au nanoclusters MnO<sub>2</sub> nanosheets alkaline phosphatase fluorescence resonance energy transfer |
| url | https://www.mdpi.com/2079-6374/15/1/49 |
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