BSA-Assisted Synthesis of Au Nanoclusters/MnO<sub>2</sub> Nanosheets for Fluorescence “Switch-On” Detection of Alkaline Phosphatase
A fluorescence probe for “switch-on” detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO<sub>2</sub> nanosheets (Au NCs-MnO<sub>2</sub> NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot a...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/15/1/49 |
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| Summary: | A fluorescence probe for “switch-on” detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO<sub>2</sub> nanosheets (Au NCs-MnO<sub>2</sub> NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot approach. In the sensing system, MnO<sub>2</sub> NSs function as both energy acceptors and target identifiers, effectively quenches the fluorescence of Au NCs via fluorescence resonance energy transfer (FRET). The presence of ALP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), reducing MnO<sub>2</sub> NSs to Mn<sup>2+</sup> and facilitate the fluorescence recovery of Au NCs. The fluorescence assay offers the advantages of facile preparation, cost-effectiveness, good specificity, and high sensitivity. Moreover, the assay exhibits a broad linear range (0.005 U/mL to 8 U/mL) for ALP detection with a remarkable limit of detection of 0.0015 U/mL. Notably, this assay demonstrates promising applicability for detection ALP in human serum samples, thereby providing valuable potential for clinical applications. |
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| ISSN: | 2079-6374 |