Characterization of liver, adipose, and fecal microbiome in obese patients with MASLD: links with disease severity and metabolic dysfunction parameters
Abstract Background Metabolic dysfunction-associated steatotic liver disease (MASLD) encompasses a range of histological findings from the generally benign simple steatosis to steatohepatitis (MASH) which can progress to fibrosis and cirrhosis. Several factors, including the microbiome, may contribu...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Microbiome |
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| Online Access: | https://doi.org/10.1186/s40168-024-02004-7 |
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| author | Katherine J. P. Schwenger Julia K. Copeland Yasaman Ghorbani Lina Chen Elena M. Comelli David S. Guttman Sandra E. Fischer Timothy D. Jackson Allan Okrainec Johane P. Allard |
| author_facet | Katherine J. P. Schwenger Julia K. Copeland Yasaman Ghorbani Lina Chen Elena M. Comelli David S. Guttman Sandra E. Fischer Timothy D. Jackson Allan Okrainec Johane P. Allard |
| author_sort | Katherine J. P. Schwenger |
| collection | DOAJ |
| description | Abstract Background Metabolic dysfunction-associated steatotic liver disease (MASLD) encompasses a range of histological findings from the generally benign simple steatosis to steatohepatitis (MASH) which can progress to fibrosis and cirrhosis. Several factors, including the microbiome, may contribute to disease progression. Results Here, we demonstrate links between the presence and abundance of specific bacteria in the adipose and liver tissues, inflammatory genes, immune cell responses, and disease severity. Overall, in MASLD patients, we observed a generalized obesity-induced translocation of gut bacteria to hepatic and adipose tissues. We identified microbial patterns unique to more severely diseased tissues. Specifically, Enterococcus, Granulicatella, and Morganellaceae abundance is positively correlated with immune cell counts and inflammatory gene expression levels, and both genera are significantly enriched in MASH patients. Brevibacterium is enriched in adipose tissues of patients with liver fibrosis. Conclusion Together, these results provide further insight into the microbial factors that may be driving disease severity. Video Abstract |
| format | Article |
| id | doaj-art-03294ef25f324584ae2e1a90f7566a51 |
| institution | Kabale University |
| issn | 2049-2618 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj-art-03294ef25f324584ae2e1a90f7566a512025-01-19T12:32:30ZengBMCMicrobiome2049-26182025-01-0113111810.1186/s40168-024-02004-7Characterization of liver, adipose, and fecal microbiome in obese patients with MASLD: links with disease severity and metabolic dysfunction parametersKatherine J. P. Schwenger0Julia K. Copeland1Yasaman Ghorbani2Lina Chen3Elena M. Comelli4David S. Guttman5Sandra E. Fischer6Timothy D. Jackson7Allan Okrainec8Johane P. Allard9Toronto General Hospital, University Health NetworkCentre for the Analysis of Genome Evolution & Function, University of TorontoToronto General Hospital, University Health NetworkSunnybrook Health Sciences Centre, University of TorontoDepartment of Nutritional Sciences, University of TorontoCentre for the Analysis of Genome Evolution & Function, University of TorontoToronto General Hospital, University Health NetworkDivision of General Surgery, University of TorontoDivision of General Surgery, University of TorontoToronto General Hospital, University Health NetworkAbstract Background Metabolic dysfunction-associated steatotic liver disease (MASLD) encompasses a range of histological findings from the generally benign simple steatosis to steatohepatitis (MASH) which can progress to fibrosis and cirrhosis. Several factors, including the microbiome, may contribute to disease progression. Results Here, we demonstrate links between the presence and abundance of specific bacteria in the adipose and liver tissues, inflammatory genes, immune cell responses, and disease severity. Overall, in MASLD patients, we observed a generalized obesity-induced translocation of gut bacteria to hepatic and adipose tissues. We identified microbial patterns unique to more severely diseased tissues. Specifically, Enterococcus, Granulicatella, and Morganellaceae abundance is positively correlated with immune cell counts and inflammatory gene expression levels, and both genera are significantly enriched in MASH patients. Brevibacterium is enriched in adipose tissues of patients with liver fibrosis. Conclusion Together, these results provide further insight into the microbial factors that may be driving disease severity. Video Abstracthttps://doi.org/10.1186/s40168-024-02004-7Fatty liverTissue microbiomeHepatic immune cellsHepatic transcriptomeAdipose gene expression |
| spellingShingle | Katherine J. P. Schwenger Julia K. Copeland Yasaman Ghorbani Lina Chen Elena M. Comelli David S. Guttman Sandra E. Fischer Timothy D. Jackson Allan Okrainec Johane P. Allard Characterization of liver, adipose, and fecal microbiome in obese patients with MASLD: links with disease severity and metabolic dysfunction parameters Microbiome Fatty liver Tissue microbiome Hepatic immune cells Hepatic transcriptome Adipose gene expression |
| title | Characterization of liver, adipose, and fecal microbiome in obese patients with MASLD: links with disease severity and metabolic dysfunction parameters |
| title_full | Characterization of liver, adipose, and fecal microbiome in obese patients with MASLD: links with disease severity and metabolic dysfunction parameters |
| title_fullStr | Characterization of liver, adipose, and fecal microbiome in obese patients with MASLD: links with disease severity and metabolic dysfunction parameters |
| title_full_unstemmed | Characterization of liver, adipose, and fecal microbiome in obese patients with MASLD: links with disease severity and metabolic dysfunction parameters |
| title_short | Characterization of liver, adipose, and fecal microbiome in obese patients with MASLD: links with disease severity and metabolic dysfunction parameters |
| title_sort | characterization of liver adipose and fecal microbiome in obese patients with masld links with disease severity and metabolic dysfunction parameters |
| topic | Fatty liver Tissue microbiome Hepatic immune cells Hepatic transcriptome Adipose gene expression |
| url | https://doi.org/10.1186/s40168-024-02004-7 |
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