Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis
A major issue with Schistosoma mansoni infection is the development of periportal fibrosis, which is predominantly caused by the host immune response to egg antigens. Experimental studies have pointed to the participation of monocytes in the pathogenesis of liver fibrosis. The aim of this study was...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/703653 |
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| author | Jamille Souza Fernandes Maria Ilma Araujo Diego Mota Lopes Robson da Paixão de Souza Edgar M. Carvalho Luciana Santos Cardoso |
| author_facet | Jamille Souza Fernandes Maria Ilma Araujo Diego Mota Lopes Robson da Paixão de Souza Edgar M. Carvalho Luciana Santos Cardoso |
| author_sort | Jamille Souza Fernandes |
| collection | DOAJ |
| description | A major issue with Schistosoma mansoni infection is the development of periportal fibrosis, which is predominantly caused by the host immune response to egg antigens. Experimental studies have pointed to the participation of monocytes in the pathogenesis of liver fibrosis. The aim of this study was to characterize the subsets of monocytes in individuals with different degrees of periportal fibrosis secondary to schistosomiasis. Monocytes were classified into classical (CD14++CD16−), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++). The expressions of monocyte markers and cytokines were assessed using flow cytometry. The frequency of classical monocytes was higher than the other subsets. The expression of HLA-DR, IL-6, TNF-α, and TGF-β was higher in monocytes from individuals with moderate to severe fibrosis as compared to other groups. Although no differences were observed in receptors expression (IL-4R and IL-10R) between groups of patients, the expression of IL-12 was lower in monocytes from individuals with moderate to severe fibrosis, suggesting a protective role of this cytokine in the development of fibrosis. Our data support the hypothesis that the three different monocyte populations participate in the immunopathogenesis of periportal fibrosis, since they express high levels of proinflammatory and profibrotic cytokines and low levels of regulatory markers. |
| format | Article |
| id | doaj-art-02e800e37ac24e57ba87bf7c3ee2488e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-02e800e37ac24e57ba87bf7c3ee2488e2025-02-03T01:32:32ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/703653703653Monocyte Subsets in Schistosomiasis Patients with Periportal FibrosisJamille Souza Fernandes0Maria Ilma Araujo1Diego Mota Lopes2Robson da Paixão de Souza3Edgar M. Carvalho4Luciana Santos Cardoso5Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilServiço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, BrazilA major issue with Schistosoma mansoni infection is the development of periportal fibrosis, which is predominantly caused by the host immune response to egg antigens. Experimental studies have pointed to the participation of monocytes in the pathogenesis of liver fibrosis. The aim of this study was to characterize the subsets of monocytes in individuals with different degrees of periportal fibrosis secondary to schistosomiasis. Monocytes were classified into classical (CD14++CD16−), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++). The expressions of monocyte markers and cytokines were assessed using flow cytometry. The frequency of classical monocytes was higher than the other subsets. The expression of HLA-DR, IL-6, TNF-α, and TGF-β was higher in monocytes from individuals with moderate to severe fibrosis as compared to other groups. Although no differences were observed in receptors expression (IL-4R and IL-10R) between groups of patients, the expression of IL-12 was lower in monocytes from individuals with moderate to severe fibrosis, suggesting a protective role of this cytokine in the development of fibrosis. Our data support the hypothesis that the three different monocyte populations participate in the immunopathogenesis of periportal fibrosis, since they express high levels of proinflammatory and profibrotic cytokines and low levels of regulatory markers.http://dx.doi.org/10.1155/2014/703653 |
| spellingShingle | Jamille Souza Fernandes Maria Ilma Araujo Diego Mota Lopes Robson da Paixão de Souza Edgar M. Carvalho Luciana Santos Cardoso Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis Mediators of Inflammation |
| title | Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis |
| title_full | Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis |
| title_fullStr | Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis |
| title_full_unstemmed | Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis |
| title_short | Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis |
| title_sort | monocyte subsets in schistosomiasis patients with periportal fibrosis |
| url | http://dx.doi.org/10.1155/2014/703653 |
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