Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila
Abstract Dayak onion (Eleutherine bulbosa (Mill.) Urb.) has the potential to be an immunostimulant to benefit fish health. This study aimed to evaluate the effectiveness of dietary supplementation with the powder and crude ethanol extracts of Dayak onion on the hematology and immune response param...
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| Format: | Article |
| Language: | English |
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Faculty of Fisheries and Marine Universitas Airlangga
2023-09-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
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| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/44079 |
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| author | Arini Resti Fauzi Munti Yuhana Widanarni Widanarni Mia Setiawati Usamah Afiff |
| author_facet | Arini Resti Fauzi Munti Yuhana Widanarni Widanarni Mia Setiawati Usamah Afiff |
| author_sort | Arini Resti Fauzi |
| collection | DOAJ |
| description | Abstract
Dayak onion (Eleutherine bulbosa (Mill.) Urb.) has the potential to be an immunostimulant to benefit fish health. This study aimed to evaluate the effectiveness of dietary supplementation with the powder and crude ethanol extracts of Dayak onion on the hematology and immune response parameters of Nile tilapia challenged with Aeromonas hydrophila. The research used a completely randomized design with six treatments and three replications. Nile tilapias were fed with Dayak onion (w/v) consisting of powders of 5% (P5), 10% (P10), and 15% (P15), while crude extract was 0.5% (E05) and given during rearing for 30 days. Positive control (C+) and negative control (C-) were used without Dayak onion. Nile tilapia in treatment C+, P5, P10, and P15 were injected with A. hydrophila 10ⶠCFU mLâ»1, while that in treatment C- was injected with phosphate buffered saline (PBS) intramuscularly. The results showed that the dietary with 15% powder and 0.5% crude extract significantly improved the hematology and immune response parameters compared to the control after the challenge against A. hydrophila (P<0.05). Dayak onion supplementation effectively increased the health status based on hematology and immune response parameters of Nile tilapia against A. hydrophila infection.
Highlight Research
• Dayak onion has significant potential to improve nonspecific immunity in fish.
• Dayak onion improves hematology and immune response of Nile tilapia.
• Dayak onion can significantly regulate the immune system against A. hydrophila infection.
• Dayak onion significantly increases the production of the gene expression of IL-1β and TNF-α. |
| format | Article |
| id | doaj-art-02d4e94e63d64a3e9669bcf8f8e7f64b |
| institution | Kabale University |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-02d4e94e63d64a3e9669bcf8f8e7f64b2025-02-03T05:30:46ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592023-09-0116111410.20473/jipk.v16i1.4407942161Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophilaArini Resti Fauzi0https://orcid.org/0000-0002-0162-6895Munti Yuhana1https://orcid.org/0000-0002-4019-0721Widanarni Widanarni2https://orcid.org/0000-0002-0821-3225Mia Setiawati3https://orcid.org/0000-0002-6604-8184Usamah Afiff4https://orcid.org/0000-0002-1373-9520Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. IndonesiaDepartment of Veterinary Disease and Public Health, Veterinary and Biomedical School, IPB University, Bogor, West Java. IndonesiaAbstract Dayak onion (Eleutherine bulbosa (Mill.) Urb.) has the potential to be an immunostimulant to benefit fish health. This study aimed to evaluate the effectiveness of dietary supplementation with the powder and crude ethanol extracts of Dayak onion on the hematology and immune response parameters of Nile tilapia challenged with Aeromonas hydrophila. The research used a completely randomized design with six treatments and three replications. Nile tilapias were fed with Dayak onion (w/v) consisting of powders of 5% (P5), 10% (P10), and 15% (P15), while crude extract was 0.5% (E05) and given during rearing for 30 days. Positive control (C+) and negative control (C-) were used without Dayak onion. Nile tilapia in treatment C+, P5, P10, and P15 were injected with A. hydrophila 10ⶠCFU mLâ»1, while that in treatment C- was injected with phosphate buffered saline (PBS) intramuscularly. The results showed that the dietary with 15% powder and 0.5% crude extract significantly improved the hematology and immune response parameters compared to the control after the challenge against A. hydrophila (P<0.05). Dayak onion supplementation effectively increased the health status based on hematology and immune response parameters of Nile tilapia against A. hydrophila infection. Highlight Research • Dayak onion has significant potential to improve nonspecific immunity in fish. • Dayak onion improves hematology and immune response of Nile tilapia. • Dayak onion can significantly regulate the immune system against A. hydrophila infection. • Dayak onion significantly increases the production of the gene expression of IL-1β and TNF-α.https://e-journal.unair.ac.id/JIPK/article/view/44079eleutherine bulbosagene expressionhistopathologyimmunostimulant |
| spellingShingle | Arini Resti Fauzi Munti Yuhana Widanarni Widanarni Mia Setiawati Usamah Afiff Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila Jurnal Ilmiah Perikanan dan Kelautan eleutherine bulbosa gene expression histopathology immunostimulant |
| title | Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila |
| title_full | Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila |
| title_fullStr | Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila |
| title_full_unstemmed | Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila |
| title_short | Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila |
| title_sort | effect of dayak onion eleutherine bulbosa mill urb on the immune response and gene expression of nile tilapia oreochromis niloticus infected with aeromonas hydrophila |
| topic | eleutherine bulbosa gene expression histopathology immunostimulant |
| url | https://e-journal.unair.ac.id/JIPK/article/view/44079 |
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