CD8 Knockout Mice Are Protected from Challenge by Vaccination with WR201, a Live Attenuated Mutant of Brucella melitensis
CD8+ T cells have been reported to play an important role in defense against B. abortus infection in mouse models. In the present report, we use CD8 knockout mice to further elucidate the role of these cells in protection from B. melitensis infection. Mice were immunized orally by administration of...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/686919 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553374789140480 |
|---|---|
| author | Samuel L. Yingst Mina Izadjoo David L. Hoover |
| author_facet | Samuel L. Yingst Mina Izadjoo David L. Hoover |
| author_sort | Samuel L. Yingst |
| collection | DOAJ |
| description | CD8+ T cells have been reported to play an important role in defense against B. abortus infection in mouse models. In the present report, we use CD8 knockout mice to further elucidate the role of these cells in protection from B. melitensis infection. Mice were immunized orally by administration of B. melitensis WR201, a purine auxotrophic attenuated vaccine strain, then challenged intranasally with B. melitensis 16M. In some experiments, persistence of WR201 in the spleens of CD8 knockout mice was slightly longer than that in the spleens of normal mice. However, development of anti-LPS serum antibody, antigen-induced production of γ-interferon (IFN-γ) by immune splenic lymphocytes, protection against intranasal challenge, and recovery of nonimmunized animals from intranasal challenge were similar between normal and knockout animals. Further, primary Brucella infection was not exacerbated in perforin knockout and Fas-deficient mice and these animals’ anti-Brucella immune responses were indistinguishable from those of normal mice. These results indicate that CD8+ T cells do not play an essential role as either cytotoxic cells or IFN-γ producers, yet they do participate in a specific immune response to immunization and challenge in this murine model of B. melitensis infection. |
| format | Article |
| id | doaj-art-02d3678ad0a74e419358e513fe46e68f |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-02d3678ad0a74e419358e513fe46e68f2025-02-03T05:54:05ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/686919686919CD8 Knockout Mice Are Protected from Challenge by Vaccination with WR201, a Live Attenuated Mutant of Brucella melitensisSamuel L. Yingst0Mina Izadjoo1David L. Hoover2Department of Bacterial Diseases, Walter Reed Army Institute of Research, Silver Spring, MD 20910-5100, USADiagnostics and Translational Research Center, Henry M. Jackson Foundation for the Advancement of Military Medicine, 401 Professional Drive, Suite 210, Gaithersburg, MD 20879, USADHMD Consulting, LLC, 13725 Drake Drive, Rockville, MD 20853, USACD8+ T cells have been reported to play an important role in defense against B. abortus infection in mouse models. In the present report, we use CD8 knockout mice to further elucidate the role of these cells in protection from B. melitensis infection. Mice were immunized orally by administration of B. melitensis WR201, a purine auxotrophic attenuated vaccine strain, then challenged intranasally with B. melitensis 16M. In some experiments, persistence of WR201 in the spleens of CD8 knockout mice was slightly longer than that in the spleens of normal mice. However, development of anti-LPS serum antibody, antigen-induced production of γ-interferon (IFN-γ) by immune splenic lymphocytes, protection against intranasal challenge, and recovery of nonimmunized animals from intranasal challenge were similar between normal and knockout animals. Further, primary Brucella infection was not exacerbated in perforin knockout and Fas-deficient mice and these animals’ anti-Brucella immune responses were indistinguishable from those of normal mice. These results indicate that CD8+ T cells do not play an essential role as either cytotoxic cells or IFN-γ producers, yet they do participate in a specific immune response to immunization and challenge in this murine model of B. melitensis infection.http://dx.doi.org/10.1155/2013/686919 |
| spellingShingle | Samuel L. Yingst Mina Izadjoo David L. Hoover CD8 Knockout Mice Are Protected from Challenge by Vaccination with WR201, a Live Attenuated Mutant of Brucella melitensis Clinical and Developmental Immunology |
| title | CD8 Knockout Mice Are Protected from Challenge by Vaccination with WR201, a Live Attenuated Mutant of Brucella melitensis |
| title_full | CD8 Knockout Mice Are Protected from Challenge by Vaccination with WR201, a Live Attenuated Mutant of Brucella melitensis |
| title_fullStr | CD8 Knockout Mice Are Protected from Challenge by Vaccination with WR201, a Live Attenuated Mutant of Brucella melitensis |
| title_full_unstemmed | CD8 Knockout Mice Are Protected from Challenge by Vaccination with WR201, a Live Attenuated Mutant of Brucella melitensis |
| title_short | CD8 Knockout Mice Are Protected from Challenge by Vaccination with WR201, a Live Attenuated Mutant of Brucella melitensis |
| title_sort | cd8 knockout mice are protected from challenge by vaccination with wr201 a live attenuated mutant of brucella melitensis |
| url | http://dx.doi.org/10.1155/2013/686919 |
| work_keys_str_mv | AT samuellyingst cd8knockoutmiceareprotectedfromchallengebyvaccinationwithwr201aliveattenuatedmutantofbrucellamelitensis AT minaizadjoo cd8knockoutmiceareprotectedfromchallengebyvaccinationwithwr201aliveattenuatedmutantofbrucellamelitensis AT davidlhoover cd8knockoutmiceareprotectedfromchallengebyvaccinationwithwr201aliveattenuatedmutantofbrucellamelitensis |