The Characterization of L-Asparaginase with Low L-Glutaminase Activity Produced by the Marine <i>Pseudomonas</i> sp. Strain GH-W2b
L-asparaginase (ASNase) hydrolyzes L-asparagine to L-aspartic acid and ammonia and has been used as an antitumor agent for the treatment of acute lymphoblastic leukemia. ASNase has also been used to mitigate the suspected carcinogenic effects of acrylamide in foods. Commercial ASNases currently used...
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2024-12-01
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| author | Woon-Jong Yu Ha Young Lee Yong Min Kwon Seung Seob Bae Grace Choi Hyun-Ju Hwang Dawoon Chung |
| author_facet | Woon-Jong Yu Ha Young Lee Yong Min Kwon Seung Seob Bae Grace Choi Hyun-Ju Hwang Dawoon Chung |
| author_sort | Woon-Jong Yu |
| collection | DOAJ |
| description | L-asparaginase (ASNase) hydrolyzes L-asparagine to L-aspartic acid and ammonia and has been used as an antitumor agent for the treatment of acute lymphoblastic leukemia. ASNase has also been used to mitigate the suspected carcinogenic effects of acrylamide in foods. Commercial ASNases currently used in the pharmaceutical and food industries are produced by microorganisms, such as bacteria and fungi. However, their toxicity and poor thermal stability limit their application. Therefore, identifying novel sources of ASNase is critical. In the present study, we identified an asparaginase-producing marine bacterial strain, GH-W2b, as a <i>Pseudomonas</i> species. Based on the plate assay results, GH-W2b produced ASNase with marginal L-glutaminase (GLNase) activity, which has been reported to cause adverse effects in clinical ASNases. The ASNase activity of GH-W2b was maximized at 50–65 °C and pH 7.0–8.5. Notably, the activities were consistent at a wide range of NaCl concentrations (0–15%) at 37 °C. In addition, compared to the control (no pre-incubation), ASNase activities were retained (>87%) by 2 h pre-incubation at 4–37 °C. Overall, our results suggest that GH-W2b ASNase has the potential to serve as a candidate for the development of salt-tolerant and/or alternative ASNases in pharmaceutical and food products. |
| format | Article |
| id | doaj-art-02d2eaaa90f14b6192d494b8b9a6b690 |
| institution | Kabale University |
| issn | 2036-7481 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microbiology Research |
| spelling | doaj-art-02d2eaaa90f14b6192d494b8b9a6b6902025-01-24T13:41:41ZengMDPI AGMicrobiology Research2036-74812024-12-01161210.3390/microbiolres16010002The Characterization of L-Asparaginase with Low L-Glutaminase Activity Produced by the Marine <i>Pseudomonas</i> sp. Strain GH-W2bWoon-Jong Yu0Ha Young Lee1Yong Min Kwon2Seung Seob Bae3Grace Choi4Hyun-Ju Hwang5Dawoon Chung6Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of KoreaDepartment of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of KoreaDepartment of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of KoreaDepartment of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of KoreaDepartment of Biomaterial Research, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of KoreaDepartment of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of KoreaDepartment of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of KoreaL-asparaginase (ASNase) hydrolyzes L-asparagine to L-aspartic acid and ammonia and has been used as an antitumor agent for the treatment of acute lymphoblastic leukemia. ASNase has also been used to mitigate the suspected carcinogenic effects of acrylamide in foods. Commercial ASNases currently used in the pharmaceutical and food industries are produced by microorganisms, such as bacteria and fungi. However, their toxicity and poor thermal stability limit their application. Therefore, identifying novel sources of ASNase is critical. In the present study, we identified an asparaginase-producing marine bacterial strain, GH-W2b, as a <i>Pseudomonas</i> species. Based on the plate assay results, GH-W2b produced ASNase with marginal L-glutaminase (GLNase) activity, which has been reported to cause adverse effects in clinical ASNases. The ASNase activity of GH-W2b was maximized at 50–65 °C and pH 7.0–8.5. Notably, the activities were consistent at a wide range of NaCl concentrations (0–15%) at 37 °C. In addition, compared to the control (no pre-incubation), ASNase activities were retained (>87%) by 2 h pre-incubation at 4–37 °C. Overall, our results suggest that GH-W2b ASNase has the potential to serve as a candidate for the development of salt-tolerant and/or alternative ASNases in pharmaceutical and food products.https://www.mdpi.com/2036-7481/16/1/2L-asparaginasea marine bacterium<i>Pseudomonas</i> sp.L-glutaminase |
| spellingShingle | Woon-Jong Yu Ha Young Lee Yong Min Kwon Seung Seob Bae Grace Choi Hyun-Ju Hwang Dawoon Chung The Characterization of L-Asparaginase with Low L-Glutaminase Activity Produced by the Marine <i>Pseudomonas</i> sp. Strain GH-W2b Microbiology Research L-asparaginase a marine bacterium <i>Pseudomonas</i> sp. L-glutaminase |
| title | The Characterization of L-Asparaginase with Low L-Glutaminase Activity Produced by the Marine <i>Pseudomonas</i> sp. Strain GH-W2b |
| title_full | The Characterization of L-Asparaginase with Low L-Glutaminase Activity Produced by the Marine <i>Pseudomonas</i> sp. Strain GH-W2b |
| title_fullStr | The Characterization of L-Asparaginase with Low L-Glutaminase Activity Produced by the Marine <i>Pseudomonas</i> sp. Strain GH-W2b |
| title_full_unstemmed | The Characterization of L-Asparaginase with Low L-Glutaminase Activity Produced by the Marine <i>Pseudomonas</i> sp. Strain GH-W2b |
| title_short | The Characterization of L-Asparaginase with Low L-Glutaminase Activity Produced by the Marine <i>Pseudomonas</i> sp. Strain GH-W2b |
| title_sort | characterization of l asparaginase with low l glutaminase activity produced by the marine i pseudomonas i sp strain gh w2b |
| topic | L-asparaginase a marine bacterium <i>Pseudomonas</i> sp. L-glutaminase |
| url | https://www.mdpi.com/2036-7481/16/1/2 |
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