Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein
Ferric ion binding proteins (Fbps) transport FeIII across the periplasm and are vital for the virulence of many Gram negative bacteria. Iron(III) is tightly bound in a hinged binding cleft with octahedral coordination geometry involving binding to protein side chains (including tyrosinate residues)...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2010/307578 |
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| _version_ | 1832553308832661504 |
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| author | Yanbo Shi Ian Harvey Dominic Campopiano Peter J. Sadler |
| author_facet | Yanbo Shi Ian Harvey Dominic Campopiano Peter J. Sadler |
| author_sort | Yanbo Shi |
| collection | DOAJ |
| description | Ferric ion binding proteins (Fbps) transport FeIII across the periplasm and are vital for the virulence of many Gram negative bacteria. Iron(III) is tightly bound in a hinged binding cleft with octahedral coordination geometry involving binding to protein side chains (including tyrosinate residues) together with a synergistic anion such as phosphate. Niobium compounds are of interest for their potential biological activity, which has been little explored. We have studied the binding of cyclopentadienyl and nitrilotriacetato NbV complexes to the Fbp from Neisseria gonorrhoeae by UV-vis spectroscopy, chromatography, ICP-OES, mass spectrometry, and Nb K-edge X-ray absorption spectroscopy. These data suggest that NbV binds strongly to Fbp and that a dinuclear NbV centre can be readily accommodated in the interdomain binding cleft. The possibility of designing niobium-based antibiotics which block iron uptake by pathogenic bacteria is discussed. |
| format | Article |
| id | doaj-art-02c0a8a984c9419aa71046ea0efe9a7a |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-02c0a8a984c9419aa71046ea0efe9a7a2025-02-03T05:54:19ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2010-01-01201010.1155/2010/307578307578Niobium Uptake and Release by Bacterial Ferric Ion Binding ProteinYanbo Shi0Ian Harvey1Dominic Campopiano2Peter J. Sadler3School of Chemistry, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JJ, UKCLRC Daresbury Laboratory, Warrington WA4 4AD, UKSchool of Chemistry, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JJ, UKDepartment of Chemistry, University of Warwick, Coventry CV4 7AL, UKFerric ion binding proteins (Fbps) transport FeIII across the periplasm and are vital for the virulence of many Gram negative bacteria. Iron(III) is tightly bound in a hinged binding cleft with octahedral coordination geometry involving binding to protein side chains (including tyrosinate residues) together with a synergistic anion such as phosphate. Niobium compounds are of interest for their potential biological activity, which has been little explored. We have studied the binding of cyclopentadienyl and nitrilotriacetato NbV complexes to the Fbp from Neisseria gonorrhoeae by UV-vis spectroscopy, chromatography, ICP-OES, mass spectrometry, and Nb K-edge X-ray absorption spectroscopy. These data suggest that NbV binds strongly to Fbp and that a dinuclear NbV centre can be readily accommodated in the interdomain binding cleft. The possibility of designing niobium-based antibiotics which block iron uptake by pathogenic bacteria is discussed.http://dx.doi.org/10.1155/2010/307578 |
| spellingShingle | Yanbo Shi Ian Harvey Dominic Campopiano Peter J. Sadler Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein Bioinorganic Chemistry and Applications |
| title | Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein |
| title_full | Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein |
| title_fullStr | Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein |
| title_full_unstemmed | Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein |
| title_short | Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein |
| title_sort | niobium uptake and release by bacterial ferric ion binding protein |
| url | http://dx.doi.org/10.1155/2010/307578 |
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