Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue
Summary: Cell competition is a quality control mechanism that promotes elimination of suboptimal cells relative to fitter neighbors. Cancer cells exploit these mechanisms for expansion, but the underlying molecular pathways remain elusive. Here, we present a protocol for generating matrix-free micro...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007408 |
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| author | Maria Lamprou Ana Krotenberg Garcia Saskia Jacoba Elisabeth Suijkerbuijk |
| author_facet | Maria Lamprou Ana Krotenberg Garcia Saskia Jacoba Elisabeth Suijkerbuijk |
| author_sort | Maria Lamprou |
| collection | DOAJ |
| description | Summary: Cell competition is a quality control mechanism that promotes elimination of suboptimal cells relative to fitter neighbors. Cancer cells exploit these mechanisms for expansion, but the underlying molecular pathways remain elusive. Here, we present a protocol for generating matrix-free microtissues recapitulating cellular interactions between intestinal cancer and hepatocyte-like cells using microscopy or transcriptomics/proteomics. We describe steps for generating and differentiating liver progenitor organoids and microtissue formation. We then detail procedures for immunofluorescence staining, mounting microtissues, and sorting cells.For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-02ba8ec7b51b4462a0cb3f59cbd9141a |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-02ba8ec7b51b4462a0cb3f59cbd9141a2025-01-21T04:13:19ZengElsevierSTAR Protocols2666-16672025-03-0161103575Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissueMaria Lamprou0Ana Krotenberg Garcia1Saskia Jacoba Elisabeth Suijkerbuijk2Division of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, Utrecht 3584 CH, the Netherlands; Corresponding authorDivision of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, Utrecht 3584 CH, the NetherlandsDivision of Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, Utrecht 3584 CH, the Netherlands; Corresponding authorSummary: Cell competition is a quality control mechanism that promotes elimination of suboptimal cells relative to fitter neighbors. Cancer cells exploit these mechanisms for expansion, but the underlying molecular pathways remain elusive. Here, we present a protocol for generating matrix-free microtissues recapitulating cellular interactions between intestinal cancer and hepatocyte-like cells using microscopy or transcriptomics/proteomics. We describe steps for generating and differentiating liver progenitor organoids and microtissue formation. We then detail procedures for immunofluorescence staining, mounting microtissues, and sorting cells.For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007408CancerMicroscopyOrganoids |
| spellingShingle | Maria Lamprou Ana Krotenberg Garcia Saskia Jacoba Elisabeth Suijkerbuijk Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue STAR Protocols Cancer Microscopy Organoids |
| title | Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue |
| title_full | Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue |
| title_fullStr | Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue |
| title_full_unstemmed | Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue |
| title_short | Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue |
| title_sort | protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue |
| topic | Cancer Microscopy Organoids |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007408 |
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