Hsa-circVIM regulates breast cancer tumor progression and tamoxifen sensitivity by sponging miR-1294 in hormone receptor-positive breast cancer cells

Abstract Background The progression and metastasis of breast cancer patients are regulated by genetics and epigenetics. Circular RNA (circRNA) plays a pivotal role in modulating the advancement of tumors. The study aimed to explore the clinical performance and regulatory role of hsa-circVIM in breas...

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Main Authors: Ying Chen, Zhenhua Zhang, Xue Luo, Meini Cen, Peng Dai, Xiaoyan Zhu
Format: Article
Language:English
Published: Springer 2025-05-01
Series:Discover Oncology
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Online Access:https://doi.org/10.1007/s12672-025-02485-4
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author Ying Chen
Zhenhua Zhang
Xue Luo
Meini Cen
Peng Dai
Xiaoyan Zhu
author_facet Ying Chen
Zhenhua Zhang
Xue Luo
Meini Cen
Peng Dai
Xiaoyan Zhu
author_sort Ying Chen
collection DOAJ
description Abstract Background The progression and metastasis of breast cancer patients are regulated by genetics and epigenetics. Circular RNA (circRNA) plays a pivotal role in modulating the advancement of tumors. The study aimed to explore the clinical performance and regulatory role of hsa-circVIM in breast cancer and its modulatory effect on tamoxifen resistance in hormone receptor (HR) positive breast cancer cells. Methods RT-qPCR was performed to detect hsa-circVIM expression in breast cancer tissues and cells.CCK-8 assay, Transwell assay, and flow cytometric analyses were performed to evaluate the effects of hsa-circVIM on cellular activities in breast cancer cells and TAM sensitivity in MCF7/TR cells. Bioinformatic analyses were conducted to make function and pathway enrichment analyses. Results hsa-circVIM expression was raised in breast cancer and predicted unsatisfactory overall survival outcomes. Silencing of hsa-circVIM suppressed cell viability, and migration capacities, while simultaneously enhancing TAM sensitivity and inducing apoptosis of HR-positive breast cancer cells by targeting miR-1294. Conclusion Elevated hsa-circVIM expression in breast cancer suggested its potential as a prognostic biomarker. hsa-circVIM functions as both a cancer-promoting molecule and a regulator of TAM responsiveness in HR-positive breast cancer cells by regulating miR-1294 expression. Therefore, hsa-circVIM serves as a potent biomarker for prognosis, plays a promoting role in breast cancer progression, and may offer a therapeutic avenue to overcome TAM resistance.
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spelling doaj-art-02b2f3a6d2d94e4e97319c0288670d8a2025-08-20T02:15:07ZengSpringerDiscover Oncology2730-60112025-05-0116111310.1007/s12672-025-02485-4Hsa-circVIM regulates breast cancer tumor progression and tamoxifen sensitivity by sponging miR-1294 in hormone receptor-positive breast cancer cellsYing Chen0Zhenhua Zhang1Xue Luo2Meini Cen3Peng Dai4Xiaoyan Zhu5Department of Thyroid and Breast Surgery, Xiamen Humanity HospitalDepartment of Breast and Thyroid Surgery, Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao UniversityThe Sixth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen Hospital (Fu Tian) of Guangzhou University of Chinese MedicineDepartment of Rehabilitation Medicine, The Affiliated Hospital of Youjiang Medical University for NationalitiesGeneral Medicine, Shanxi Provincial Cancer Hospital, Chinese Academy of Medical Sciences Cancer Hospital Shanxi Hospital, Shanxi Medical University Affiliated Cancer HospitalA Ward of Medical Oncology, Eastern Theater Command General Hospital Qinhuai Medical DistrictAbstract Background The progression and metastasis of breast cancer patients are regulated by genetics and epigenetics. Circular RNA (circRNA) plays a pivotal role in modulating the advancement of tumors. The study aimed to explore the clinical performance and regulatory role of hsa-circVIM in breast cancer and its modulatory effect on tamoxifen resistance in hormone receptor (HR) positive breast cancer cells. Methods RT-qPCR was performed to detect hsa-circVIM expression in breast cancer tissues and cells.CCK-8 assay, Transwell assay, and flow cytometric analyses were performed to evaluate the effects of hsa-circVIM on cellular activities in breast cancer cells and TAM sensitivity in MCF7/TR cells. Bioinformatic analyses were conducted to make function and pathway enrichment analyses. Results hsa-circVIM expression was raised in breast cancer and predicted unsatisfactory overall survival outcomes. Silencing of hsa-circVIM suppressed cell viability, and migration capacities, while simultaneously enhancing TAM sensitivity and inducing apoptosis of HR-positive breast cancer cells by targeting miR-1294. Conclusion Elevated hsa-circVIM expression in breast cancer suggested its potential as a prognostic biomarker. hsa-circVIM functions as both a cancer-promoting molecule and a regulator of TAM responsiveness in HR-positive breast cancer cells by regulating miR-1294 expression. Therefore, hsa-circVIM serves as a potent biomarker for prognosis, plays a promoting role in breast cancer progression, and may offer a therapeutic avenue to overcome TAM resistance.https://doi.org/10.1007/s12672-025-02485-4Hsa_circ_0017873TAMPrognosisProliferationInvasion
spellingShingle Ying Chen
Zhenhua Zhang
Xue Luo
Meini Cen
Peng Dai
Xiaoyan Zhu
Hsa-circVIM regulates breast cancer tumor progression and tamoxifen sensitivity by sponging miR-1294 in hormone receptor-positive breast cancer cells
Discover Oncology
Hsa_circ_0017873
TAM
Prognosis
Proliferation
Invasion
title Hsa-circVIM regulates breast cancer tumor progression and tamoxifen sensitivity by sponging miR-1294 in hormone receptor-positive breast cancer cells
title_full Hsa-circVIM regulates breast cancer tumor progression and tamoxifen sensitivity by sponging miR-1294 in hormone receptor-positive breast cancer cells
title_fullStr Hsa-circVIM regulates breast cancer tumor progression and tamoxifen sensitivity by sponging miR-1294 in hormone receptor-positive breast cancer cells
title_full_unstemmed Hsa-circVIM regulates breast cancer tumor progression and tamoxifen sensitivity by sponging miR-1294 in hormone receptor-positive breast cancer cells
title_short Hsa-circVIM regulates breast cancer tumor progression and tamoxifen sensitivity by sponging miR-1294 in hormone receptor-positive breast cancer cells
title_sort hsa circvim regulates breast cancer tumor progression and tamoxifen sensitivity by sponging mir 1294 in hormone receptor positive breast cancer cells
topic Hsa_circ_0017873
TAM
Prognosis
Proliferation
Invasion
url https://doi.org/10.1007/s12672-025-02485-4
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