Prevalence and molecular characterization of Enterocytozoon bieneusi in endangered Eld’s deer (Rucervus eldii) in Hainan, China
IntroductionEnterocytozoon bieneusi is one of the most frequent microsporidia species causing digestive disorder mainly diarrhea in humans and animals. Eld’s deer (Rucervus eldii) is the class I national key protected wildlife and only distributed on Hainan Island in China. No report on the prevalen...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Veterinary Science |
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| author | Yun Zhang Yun Zhang Guangxu Ren Guangxu Ren Qingqing Lu Jiaqi Li Jiaqi Li Yu Qiang Youyou Li Xiuyi Lai Yuan Wang Xingyue Yu Sheng Lei Yu Li Yunxing Chang Xianrong Liu Xuning Qi Zhi Xie Tingting Li Jiang Du Rui Duan Xinyu Chang Hesheng Wang Hesheng Wang Gang Lu Gang Lu |
| author_facet | Yun Zhang Yun Zhang Guangxu Ren Guangxu Ren Qingqing Lu Jiaqi Li Jiaqi Li Yu Qiang Youyou Li Xiuyi Lai Yuan Wang Xingyue Yu Sheng Lei Yu Li Yunxing Chang Xianrong Liu Xuning Qi Zhi Xie Tingting Li Jiang Du Rui Duan Xinyu Chang Hesheng Wang Hesheng Wang Gang Lu Gang Lu |
| author_sort | Yun Zhang |
| collection | DOAJ |
| description | IntroductionEnterocytozoon bieneusi is one of the most frequent microsporidia species causing digestive disorder mainly diarrhea in humans and animals. Eld’s deer (Rucervus eldii) is the class I national key protected wildlife and only distributed on Hainan Island in China. No report on the prevalence and molecular characterization of E. bieneusi in wild Eld’s deer worldwide.Methods217 fecal samples were collected from Eld’s deer in two isolated habitats of a nature reserve in Hainan, and examined by nested Polymerase Chain Reaction (PCR) targeting the internal transcribed spacer (ITS) region.Results and discussionThe overall prevalence of E. bieneusi in Eld’s deer was 17.5% (38/217), with 13.5% (12/89) and 20.3% (26/128) in habitats 1 and 2, respectively. Seven ITS genotypes were identified, including five known genotypes: D (n = 19), Peru11 (n = 10), EbpC (n = 5), Peru8 (n = 1) and Type IV (n = 1), and two novel genotypes: HNED-I and HNED-II (one each). Genotypes Peru8 and Peru11 were firstly identified in cervids. Phylogenetic analysis showed that all the detected genotypes belonged to zoonotic Group 1. The results implied that the further research on threaten of E. bieneusi to endangered Eld’s deer and potential risks for public health is necessary. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-02aa9f7717d94039bce15db6d75f26212025-01-27T06:41:02ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011210.3389/fvets.2025.15210551521055Prevalence and molecular characterization of Enterocytozoon bieneusi in endangered Eld’s deer (Rucervus eldii) in Hainan, ChinaYun Zhang0Yun Zhang1Guangxu Ren2Guangxu Ren3Qingqing Lu4Jiaqi Li5Jiaqi Li6Yu Qiang7Youyou Li8Xiuyi Lai9Yuan Wang10Xingyue Yu11Sheng Lei12Yu Li13Yunxing Chang14Xianrong Liu15Xuning Qi16Zhi Xie17Tingting Li18Jiang Du19Rui Duan20Xinyu Chang21Hesheng Wang22Hesheng Wang23Gang Lu24Gang Lu25Department of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Tropical Diseases, The Second Affiliated Hospital of Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Infection Control, The First Affiliated Hospital of USTC, Hefei, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Nuclear Medicine, The 928th Hospital of PLA Joint Logistics Force, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Nuclear Medicine, The 928th Hospital of PLA Joint Logistics Force, Haikou, ChinaHainan Bangxi Provincial Nature Reserve Administration, Baisha, ChinaHainan Bangxi Provincial Nature Reserve Administration, Baisha, ChinaBawangling Branch of Hainan Tropical Rainforest National Park Administration, Changjiang, ChinaBawangling Branch of Hainan Tropical Rainforest National Park Administration, Changjiang, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaHainan Bangxi Provincial Nature Reserve Administration, Baisha, ChinaHainan Datian National Nature Reserve Administration, Dongfang, ChinaDepartment of Pathogenic Biology, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Tropical Diseases, The Second Affiliated Hospital of Hainan Medical University, Haikou, ChinaIntroductionEnterocytozoon bieneusi is one of the most frequent microsporidia species causing digestive disorder mainly diarrhea in humans and animals. Eld’s deer (Rucervus eldii) is the class I national key protected wildlife and only distributed on Hainan Island in China. No report on the prevalence and molecular characterization of E. bieneusi in wild Eld’s deer worldwide.Methods217 fecal samples were collected from Eld’s deer in two isolated habitats of a nature reserve in Hainan, and examined by nested Polymerase Chain Reaction (PCR) targeting the internal transcribed spacer (ITS) region.Results and discussionThe overall prevalence of E. bieneusi in Eld’s deer was 17.5% (38/217), with 13.5% (12/89) and 20.3% (26/128) in habitats 1 and 2, respectively. Seven ITS genotypes were identified, including five known genotypes: D (n = 19), Peru11 (n = 10), EbpC (n = 5), Peru8 (n = 1) and Type IV (n = 1), and two novel genotypes: HNED-I and HNED-II (one each). Genotypes Peru8 and Peru11 were firstly identified in cervids. Phylogenetic analysis showed that all the detected genotypes belonged to zoonotic Group 1. The results implied that the further research on threaten of E. bieneusi to endangered Eld’s deer and potential risks for public health is necessary.https://www.frontiersin.org/articles/10.3389/fvets.2025.1521055/fullEld’s deerEnterocytozoon bieneusiprevalencegenotypeHainan Island |
| spellingShingle | Yun Zhang Yun Zhang Guangxu Ren Guangxu Ren Qingqing Lu Jiaqi Li Jiaqi Li Yu Qiang Youyou Li Xiuyi Lai Yuan Wang Xingyue Yu Sheng Lei Yu Li Yunxing Chang Xianrong Liu Xuning Qi Zhi Xie Tingting Li Jiang Du Rui Duan Xinyu Chang Hesheng Wang Hesheng Wang Gang Lu Gang Lu Prevalence and molecular characterization of Enterocytozoon bieneusi in endangered Eld’s deer (Rucervus eldii) in Hainan, China Frontiers in Veterinary Science Eld’s deer Enterocytozoon bieneusi prevalence genotype Hainan Island |
| title | Prevalence and molecular characterization of Enterocytozoon bieneusi in endangered Eld’s deer (Rucervus eldii) in Hainan, China |
| title_full | Prevalence and molecular characterization of Enterocytozoon bieneusi in endangered Eld’s deer (Rucervus eldii) in Hainan, China |
| title_fullStr | Prevalence and molecular characterization of Enterocytozoon bieneusi in endangered Eld’s deer (Rucervus eldii) in Hainan, China |
| title_full_unstemmed | Prevalence and molecular characterization of Enterocytozoon bieneusi in endangered Eld’s deer (Rucervus eldii) in Hainan, China |
| title_short | Prevalence and molecular characterization of Enterocytozoon bieneusi in endangered Eld’s deer (Rucervus eldii) in Hainan, China |
| title_sort | prevalence and molecular characterization of enterocytozoon bieneusi in endangered eld s deer rucervus eldii in hainan china |
| topic | Eld’s deer Enterocytozoon bieneusi prevalence genotype Hainan Island |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1521055/full |
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