The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in Bacteria
Biogenesis of prokaryotic molybdoenzymes is a complex process with the final step representing the insertion of a matured molybdenum cofactor (Moco) into a folded apoenzyme. Usually, specific chaperones of the XdhC family are required for the maturation of molybdoenzymes of the xanthine oxidase fami...
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| Format: | Article |
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Wiley
2011-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2011/850924 |
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| author | Meina Neumann Silke Leimkühler |
| author_facet | Meina Neumann Silke Leimkühler |
| author_sort | Meina Neumann |
| collection | DOAJ |
| description | Biogenesis of prokaryotic molybdoenzymes is a complex process with the final step representing the insertion of a matured molybdenum cofactor (Moco) into a folded apoenzyme. Usually, specific chaperones of the XdhC family are required for the maturation of molybdoenzymes of the xanthine oxidase family in bacteria. Enzymes of the xanthine oxidase family are characterized to contain an equatorial sulfur ligand at the molybdenum center of Moco. This sulfur ligand is inserted into Moco while bound to the XdhC-like protein and before its insertion into the target enzyme. In addition, enzymes of the xanthine oxidase family bind either the molybdopterin (Mo-MPT) form of Moco or the modified molybdopterin cytosine dinucleotide cofactor (MCD). In both cases, only the matured cofactor is inserted by a proofreading process of XdhC. The roles of these specific XdhC-like chaperones during the biogenesis of enzymes of the xanthine oxidase family in bacteria are described. |
| format | Article |
| id | doaj-art-02a6360787894b8c888d1a97e6b4ae7f |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
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| series | Biochemistry Research International |
| spelling | doaj-art-02a6360787894b8c888d1a97e6b4ae7f2025-02-03T01:26:13ZengWileyBiochemistry Research International2090-22472090-22552011-01-01201110.1155/2011/850924850924The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in BacteriaMeina Neumann0Silke Leimkühler1Department of Molecular Enzymology, Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, GermanyDepartment of Molecular Enzymology, Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, GermanyBiogenesis of prokaryotic molybdoenzymes is a complex process with the final step representing the insertion of a matured molybdenum cofactor (Moco) into a folded apoenzyme. Usually, specific chaperones of the XdhC family are required for the maturation of molybdoenzymes of the xanthine oxidase family in bacteria. Enzymes of the xanthine oxidase family are characterized to contain an equatorial sulfur ligand at the molybdenum center of Moco. This sulfur ligand is inserted into Moco while bound to the XdhC-like protein and before its insertion into the target enzyme. In addition, enzymes of the xanthine oxidase family bind either the molybdopterin (Mo-MPT) form of Moco or the modified molybdopterin cytosine dinucleotide cofactor (MCD). In both cases, only the matured cofactor is inserted by a proofreading process of XdhC. The roles of these specific XdhC-like chaperones during the biogenesis of enzymes of the xanthine oxidase family in bacteria are described.http://dx.doi.org/10.1155/2011/850924 |
| spellingShingle | Meina Neumann Silke Leimkühler The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in Bacteria Biochemistry Research International |
| title | The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in Bacteria |
| title_full | The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in Bacteria |
| title_fullStr | The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in Bacteria |
| title_full_unstemmed | The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in Bacteria |
| title_short | The Role of System-Specific Molecular Chaperones in the Maturation of Molybdoenzymes in Bacteria |
| title_sort | role of system specific molecular chaperones in the maturation of molybdoenzymes in bacteria |
| url | http://dx.doi.org/10.1155/2011/850924 |
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