Precise Construction of Dual-Promising Anticancer Drugs Associated with Gold Nanomaterials on Glioma Cancer Cells
Multiple chemodrugs with nanotechnology have proven to be an effective cancer treatment technique. When taken combined, cabazitaxel (CTX) and cisplatin (PT) have more excellent cytotoxic effects than drugs used alone in the chemotherapy of several different cancers. However, several severe side effe...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2023/8892099 |
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| author | P. Baby Shakila Abdurahman Hajinur Hirad Abdullah A. Alarfaj Samer Hasan Hussein-Al-Ali Beza Mulugeta |
| author_facet | P. Baby Shakila Abdurahman Hajinur Hirad Abdullah A. Alarfaj Samer Hasan Hussein-Al-Ali Beza Mulugeta |
| author_sort | P. Baby Shakila |
| collection | DOAJ |
| description | Multiple chemodrugs with nanotechnology have proven to be an effective cancer treatment technique. When taken combined, cabazitaxel (CTX) and cisplatin (PT) have more excellent cytotoxic effects than drugs used alone in the chemotherapy of several different cancers. However, several severe side effects are associated with using these chemotherapy drugs in cancer patients. Gold nanomaterials (AuNMs) are promising as drug carriers because of their small diameter, easy surface modifications, good biocompatibility, and strong cell penetration. This work aimed to determine the CTX and PT encapsulated with AuNMs against human glioma U87 cancer cells. The fabrication of the AuNMs achieved a negative surface charge, polydispersity index, and the mean sizes. The combined cytotoxic effect of CTX and PT bound to AuNMs was greater than that of either drug alone when tested on U87 cells. The half inhibitory concentration (IC50) values for free PT were 54.7 μg/mL (at 24 h) and 4.8 g μg/mL (at 72 h). Results acquired from the MTT assay show cell growth decreases time- and concentration-dependent AuNMs, free CTX, free PT, and AuNMs@CTX/PT-induced cytotoxicity and, ultimately, the cell death of U87 cells via apoptosis. The biochemical apoptosis staining techniques investigated the cells’ morphological changes of the cells (acridine orange and ethidium bromide (AO-EB) and nuclear staining (DAPI) techniques). The AO-EB and nuclear staining results reveal that the NPs effectively killed cancer cells. Furthermore, the flow cytometry analysis examined the mode of cell death. Therefore, AuNMs@CTX/PT has excellent potential in the cancer therapy of different cancer cells. |
| format | Article |
| id | doaj-art-028c0f8631dc4197aa080078a252c857 |
| institution | Kabale University |
| issn | 1687-479X |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-028c0f8631dc4197aa080078a252c8572025-02-03T06:42:54ZengWileyBioinorganic Chemistry and Applications1687-479X2023-01-01202310.1155/2023/8892099Precise Construction of Dual-Promising Anticancer Drugs Associated with Gold Nanomaterials on Glioma Cancer CellsP. Baby Shakila0Abdurahman Hajinur Hirad1Abdullah A. Alarfaj2Samer Hasan Hussein-Al-Ali3Beza Mulugeta4Department of BiochemistryDepartment of Botany and MicrobiologyDepartment of Botany and MicrobiologyDepartment of ChemistryDepartment of Food Science and Postharvest TechnologyMultiple chemodrugs with nanotechnology have proven to be an effective cancer treatment technique. When taken combined, cabazitaxel (CTX) and cisplatin (PT) have more excellent cytotoxic effects than drugs used alone in the chemotherapy of several different cancers. However, several severe side effects are associated with using these chemotherapy drugs in cancer patients. Gold nanomaterials (AuNMs) are promising as drug carriers because of their small diameter, easy surface modifications, good biocompatibility, and strong cell penetration. This work aimed to determine the CTX and PT encapsulated with AuNMs against human glioma U87 cancer cells. The fabrication of the AuNMs achieved a negative surface charge, polydispersity index, and the mean sizes. The combined cytotoxic effect of CTX and PT bound to AuNMs was greater than that of either drug alone when tested on U87 cells. The half inhibitory concentration (IC50) values for free PT were 54.7 μg/mL (at 24 h) and 4.8 g μg/mL (at 72 h). Results acquired from the MTT assay show cell growth decreases time- and concentration-dependent AuNMs, free CTX, free PT, and AuNMs@CTX/PT-induced cytotoxicity and, ultimately, the cell death of U87 cells via apoptosis. The biochemical apoptosis staining techniques investigated the cells’ morphological changes of the cells (acridine orange and ethidium bromide (AO-EB) and nuclear staining (DAPI) techniques). The AO-EB and nuclear staining results reveal that the NPs effectively killed cancer cells. Furthermore, the flow cytometry analysis examined the mode of cell death. Therefore, AuNMs@CTX/PT has excellent potential in the cancer therapy of different cancer cells.http://dx.doi.org/10.1155/2023/8892099 |
| spellingShingle | P. Baby Shakila Abdurahman Hajinur Hirad Abdullah A. Alarfaj Samer Hasan Hussein-Al-Ali Beza Mulugeta Precise Construction of Dual-Promising Anticancer Drugs Associated with Gold Nanomaterials on Glioma Cancer Cells Bioinorganic Chemistry and Applications |
| title | Precise Construction of Dual-Promising Anticancer Drugs Associated with Gold Nanomaterials on Glioma Cancer Cells |
| title_full | Precise Construction of Dual-Promising Anticancer Drugs Associated with Gold Nanomaterials on Glioma Cancer Cells |
| title_fullStr | Precise Construction of Dual-Promising Anticancer Drugs Associated with Gold Nanomaterials on Glioma Cancer Cells |
| title_full_unstemmed | Precise Construction of Dual-Promising Anticancer Drugs Associated with Gold Nanomaterials on Glioma Cancer Cells |
| title_short | Precise Construction of Dual-Promising Anticancer Drugs Associated with Gold Nanomaterials on Glioma Cancer Cells |
| title_sort | precise construction of dual promising anticancer drugs associated with gold nanomaterials on glioma cancer cells |
| url | http://dx.doi.org/10.1155/2023/8892099 |
| work_keys_str_mv | AT pbabyshakila preciseconstructionofdualpromisinganticancerdrugsassociatedwithgoldnanomaterialsongliomacancercells AT abdurahmanhajinurhirad preciseconstructionofdualpromisinganticancerdrugsassociatedwithgoldnanomaterialsongliomacancercells AT abdullahaalarfaj preciseconstructionofdualpromisinganticancerdrugsassociatedwithgoldnanomaterialsongliomacancercells AT samerhasanhusseinalali preciseconstructionofdualpromisinganticancerdrugsassociatedwithgoldnanomaterialsongliomacancercells AT bezamulugeta preciseconstructionofdualpromisinganticancerdrugsassociatedwithgoldnanomaterialsongliomacancercells |