Comparative Mitogenomics in the Genus <i>Chlorophorus</i> (Coleoptera: Cerambycidae) and Its Phylogenetic Implications
<i>Chlorophorus</i> Chevrolat, 1863, one of the most species-rich genera of Clytini, comprises 36 subgenera and 302 species/subspecies, with some species being of significant economic importance. To assess the monophyly and subgeneric system of this genus, we newly obtained mitochondrial...
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| author | Zhengju Fu Lu Chen Lichao Tian Zongqing Wang Zhu Li |
| author_facet | Zhengju Fu Lu Chen Lichao Tian Zongqing Wang Zhu Li |
| author_sort | Zhengju Fu |
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| description | <i>Chlorophorus</i> Chevrolat, 1863, one of the most species-rich genera of Clytini, comprises 36 subgenera and 302 species/subspecies, with some species being of significant economic importance. To assess the monophyly and subgeneric system of this genus, we newly obtained mitochondrial genomic data from 21 species of <i>Chlorophorus</i> via high-throughput sequencing and reconstructed the phylogeny of this genus using ML and BI methods. The mitochondrial genomes of all sequenced <i>Chlorophorus</i> species were found to comprise 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one non-coding region (control region, CR), reflecting a highly conserved gene arrangement. The mitochondrial genomes of the 21 <i>Chlorophorus</i> species range from 15,387 bp to 15,779 bp in length, and all exhibited AT bias. Analysis of the non-synonymous and synonymous substitution rates revealed that <i>ATP8</i> has the highest evolution rate, whereas <i>COI</i> has the lowest. Further, phylogenetic analyses were performed based on different datasets (13 PCGs + 2 rRNAs, 13 PCGs + 2 rRNAs + 22 tRNAs, PCG123, PCG12) using maximum likelihood (ML) and Bayesian inference (BI) methods. The results indicate that <i>Chlorophorus</i> is monophyletic, but the subgenera <i>Humeromaculatus</i> Özdikmen, 2011; <i>Immaculatoides</i> Özdikmen, 2022; <i>Brevenotatus</i> Özdikmen, 2022; and <i>Chlorophorus</i> (s. str.) Chevrolat, 1863, are not monophyletic. Based on the comparison of male genitalia, we found that the sclerites of the endophallus may exhibit phylogenetic signals for the genus. |
| format | Article |
| id | doaj-art-0288c2a6a41c4e9a840f2a77ce2bab25 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| series | Insects |
| spelling | doaj-art-0288c2a6a41c4e9a840f2a77ce2bab252025-01-24T13:35:34ZengMDPI AGInsects2075-44502024-12-01161810.3390/insects16010008Comparative Mitogenomics in the Genus <i>Chlorophorus</i> (Coleoptera: Cerambycidae) and Its Phylogenetic ImplicationsZhengju Fu0Lu Chen1Lichao Tian2Zongqing Wang3Zhu Li4College of Plant Protection, Southwest University, Chongqing 400715, ChinaCollege of Plant Protection, Southwest University, Chongqing 400715, ChinaChongqing Landscape and Gardening Research Institute, Chongqing 401329, ChinaCollege of Plant Protection, Southwest University, Chongqing 400715, ChinaCollege of Plant Protection, Southwest University, Chongqing 400715, China<i>Chlorophorus</i> Chevrolat, 1863, one of the most species-rich genera of Clytini, comprises 36 subgenera and 302 species/subspecies, with some species being of significant economic importance. To assess the monophyly and subgeneric system of this genus, we newly obtained mitochondrial genomic data from 21 species of <i>Chlorophorus</i> via high-throughput sequencing and reconstructed the phylogeny of this genus using ML and BI methods. The mitochondrial genomes of all sequenced <i>Chlorophorus</i> species were found to comprise 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one non-coding region (control region, CR), reflecting a highly conserved gene arrangement. The mitochondrial genomes of the 21 <i>Chlorophorus</i> species range from 15,387 bp to 15,779 bp in length, and all exhibited AT bias. Analysis of the non-synonymous and synonymous substitution rates revealed that <i>ATP8</i> has the highest evolution rate, whereas <i>COI</i> has the lowest. Further, phylogenetic analyses were performed based on different datasets (13 PCGs + 2 rRNAs, 13 PCGs + 2 rRNAs + 22 tRNAs, PCG123, PCG12) using maximum likelihood (ML) and Bayesian inference (BI) methods. The results indicate that <i>Chlorophorus</i> is monophyletic, but the subgenera <i>Humeromaculatus</i> Özdikmen, 2011; <i>Immaculatoides</i> Özdikmen, 2022; <i>Brevenotatus</i> Özdikmen, 2022; and <i>Chlorophorus</i> (s. str.) Chevrolat, 1863, are not monophyletic. Based on the comparison of male genitalia, we found that the sclerites of the endophallus may exhibit phylogenetic signals for the genus.https://www.mdpi.com/2075-4450/16/1/8Clytini<i>Chlorophorus</i>mitochondrial genomephylogenymonophyly |
| spellingShingle | Zhengju Fu Lu Chen Lichao Tian Zongqing Wang Zhu Li Comparative Mitogenomics in the Genus <i>Chlorophorus</i> (Coleoptera: Cerambycidae) and Its Phylogenetic Implications Insects Clytini <i>Chlorophorus</i> mitochondrial genome phylogeny monophyly |
| title | Comparative Mitogenomics in the Genus <i>Chlorophorus</i> (Coleoptera: Cerambycidae) and Its Phylogenetic Implications |
| title_full | Comparative Mitogenomics in the Genus <i>Chlorophorus</i> (Coleoptera: Cerambycidae) and Its Phylogenetic Implications |
| title_fullStr | Comparative Mitogenomics in the Genus <i>Chlorophorus</i> (Coleoptera: Cerambycidae) and Its Phylogenetic Implications |
| title_full_unstemmed | Comparative Mitogenomics in the Genus <i>Chlorophorus</i> (Coleoptera: Cerambycidae) and Its Phylogenetic Implications |
| title_short | Comparative Mitogenomics in the Genus <i>Chlorophorus</i> (Coleoptera: Cerambycidae) and Its Phylogenetic Implications |
| title_sort | comparative mitogenomics in the genus i chlorophorus i coleoptera cerambycidae and its phylogenetic implications |
| topic | Clytini <i>Chlorophorus</i> mitochondrial genome phylogeny monophyly |
| url | https://www.mdpi.com/2075-4450/16/1/8 |
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