Enhanced effects of slowly co-released TGF-β3 and BMP-2 from biomimetic calcium phosphate-coated silk fibroin scaffolds in the repair of osteochondral defects
Abstract Bioactive agents have demonstrated regenerative potential for cell-free bone tissue engineering. Nevertheless, certain challenges persist, including ineffective delivery methods and confined therapeutic potency. Here, we demonstrated that the biomimetic calcium phosphate coating system (Bio...
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BMC
2024-07-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-024-02712-0 |
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| author | Jiping Chen Yanyi Wang Tianyi Tang Baochao Li Banani Kundu Subhas C. Kundu Rui L. Reis Xingnan Lin Huang Li |
| author_facet | Jiping Chen Yanyi Wang Tianyi Tang Baochao Li Banani Kundu Subhas C. Kundu Rui L. Reis Xingnan Lin Huang Li |
| author_sort | Jiping Chen |
| collection | DOAJ |
| description | Abstract Bioactive agents have demonstrated regenerative potential for cell-free bone tissue engineering. Nevertheless, certain challenges persist, including ineffective delivery methods and confined therapeutic potency. Here, we demonstrated that the biomimetic calcium phosphate coating system (BioCaP) could effectively uptake and slowly release the incorporated bioactive agents compared to the surface absorption system via osteoclast-mediated degradation of BioCaP coatings. The release kinetics were determined as a function of time. The release rate was stable without remarkable burst release during the first 1 day, followed by a sustained release from day 7 to day 19. Then, we developed the bi-functional BioCaP-coated silk fibroin scaffolds enabling the effective co-delivery of TGF-β3 and BMP-2 (SFI-T/SFI-B) and the corresponding slow release of TGF-β3 and BMP-2 exhibited superior potential in promoting chondrogenesis and osteogenesis without impairing cell vitality in vitro. The SFI-T/SFI-B scaffolds could improve cartilage and bone regeneration in 5 × 4 mm rabbit osteochondral (OC) defect. These findings indicate that the biomimetic calcium-phosphate coated silk fibroin scaffolds with slowly co-released TGF-β3 and BMP-2 effectively promote the repair of OC defects, hence facilitating the future clinical translation of controlled drug delivery in tissue engineering. Graphic Abstract |
| format | Article |
| id | doaj-art-027dbf00c0924fa89199c6a68df704cf |
| institution | Kabale University |
| issn | 1477-3155 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-027dbf00c0924fa89199c6a68df704cf2025-02-02T12:40:56ZengBMCJournal of Nanobiotechnology1477-31552024-07-0122112110.1186/s12951-024-02712-0Enhanced effects of slowly co-released TGF-β3 and BMP-2 from biomimetic calcium phosphate-coated silk fibroin scaffolds in the repair of osteochondral defectsJiping Chen0Yanyi Wang1Tianyi Tang2Baochao Li3Banani Kundu4Subhas C. Kundu5Rui L. Reis6Xingnan Lin7Huang Li8Orthodontic Department, Nanjing Stomatological Hospital, Affiliated hospital of Medical School, Institute of Stomatology, Nanjing UniversityOrthodontic Department, Nanjing Stomatological Hospital, Affiliated hospital of Medical School, Institute of Stomatology, Nanjing UniversityDivision of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, Royal Free HospitalOrthodontic Department, Nanjing Stomatological Hospital, Affiliated hospital of Medical School, Institute of Stomatology, Nanjing University3B’s Research Group, I3Bs-Research Institute On Biomaterials, Biodegradables and Biomimetics of University of Minho, Headquarters of the European Institute of Excellence On Tissue Engineering and Regenerative Medicine3B’s Research Group, I3Bs-Research Institute On Biomaterials, Biodegradables and Biomimetics of University of Minho, Headquarters of the European Institute of Excellence On Tissue Engineering and Regenerative Medicine3B’s Research Group, I3Bs-Research Institute On Biomaterials, Biodegradables and Biomimetics of University of Minho, Headquarters of the European Institute of Excellence On Tissue Engineering and Regenerative MedicineSchool/Hospital of Stomatology, Zhejiang Chinese Medical UniversityOrthodontic Department, Nanjing Stomatological Hospital, Affiliated hospital of Medical School, Institute of Stomatology, Nanjing UniversityAbstract Bioactive agents have demonstrated regenerative potential for cell-free bone tissue engineering. Nevertheless, certain challenges persist, including ineffective delivery methods and confined therapeutic potency. Here, we demonstrated that the biomimetic calcium phosphate coating system (BioCaP) could effectively uptake and slowly release the incorporated bioactive agents compared to the surface absorption system via osteoclast-mediated degradation of BioCaP coatings. The release kinetics were determined as a function of time. The release rate was stable without remarkable burst release during the first 1 day, followed by a sustained release from day 7 to day 19. Then, we developed the bi-functional BioCaP-coated silk fibroin scaffolds enabling the effective co-delivery of TGF-β3 and BMP-2 (SFI-T/SFI-B) and the corresponding slow release of TGF-β3 and BMP-2 exhibited superior potential in promoting chondrogenesis and osteogenesis without impairing cell vitality in vitro. The SFI-T/SFI-B scaffolds could improve cartilage and bone regeneration in 5 × 4 mm rabbit osteochondral (OC) defect. These findings indicate that the biomimetic calcium-phosphate coated silk fibroin scaffolds with slowly co-released TGF-β3 and BMP-2 effectively promote the repair of OC defects, hence facilitating the future clinical translation of controlled drug delivery in tissue engineering. Graphic Abstracthttps://doi.org/10.1186/s12951-024-02712-0Silk fibroinBiomimetic calcium phosphate coatingDrug deliverySlow releaseOsteochondral defectTissue engineering |
| spellingShingle | Jiping Chen Yanyi Wang Tianyi Tang Baochao Li Banani Kundu Subhas C. Kundu Rui L. Reis Xingnan Lin Huang Li Enhanced effects of slowly co-released TGF-β3 and BMP-2 from biomimetic calcium phosphate-coated silk fibroin scaffolds in the repair of osteochondral defects Journal of Nanobiotechnology Silk fibroin Biomimetic calcium phosphate coating Drug delivery Slow release Osteochondral defect Tissue engineering |
| title | Enhanced effects of slowly co-released TGF-β3 and BMP-2 from biomimetic calcium phosphate-coated silk fibroin scaffolds in the repair of osteochondral defects |
| title_full | Enhanced effects of slowly co-released TGF-β3 and BMP-2 from biomimetic calcium phosphate-coated silk fibroin scaffolds in the repair of osteochondral defects |
| title_fullStr | Enhanced effects of slowly co-released TGF-β3 and BMP-2 from biomimetic calcium phosphate-coated silk fibroin scaffolds in the repair of osteochondral defects |
| title_full_unstemmed | Enhanced effects of slowly co-released TGF-β3 and BMP-2 from biomimetic calcium phosphate-coated silk fibroin scaffolds in the repair of osteochondral defects |
| title_short | Enhanced effects of slowly co-released TGF-β3 and BMP-2 from biomimetic calcium phosphate-coated silk fibroin scaffolds in the repair of osteochondral defects |
| title_sort | enhanced effects of slowly co released tgf β3 and bmp 2 from biomimetic calcium phosphate coated silk fibroin scaffolds in the repair of osteochondral defects |
| topic | Silk fibroin Biomimetic calcium phosphate coating Drug delivery Slow release Osteochondral defect Tissue engineering |
| url | https://doi.org/10.1186/s12951-024-02712-0 |
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