A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic Persistence
Trophoblast stem cells (TSCs) represent the multipotent progenitors that give rise to the different cells of the embryonic portion of the placenta. Here, we analysed the expression of key TSC transcription factors Cdx2, Eomes, and Elf5 in the early developing placenta of mouse embryos and in culture...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/218518 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567630897086464 |
|---|---|
| author | Georg Kuales Matthias Weiss Oliver Sedelmeier Dietmar Pfeifer Sebastian J. Arnold |
| author_facet | Georg Kuales Matthias Weiss Oliver Sedelmeier Dietmar Pfeifer Sebastian J. Arnold |
| author_sort | Georg Kuales |
| collection | DOAJ |
| description | Trophoblast stem cells (TSCs) represent the multipotent progenitors that give rise to the different cells of the embryonic portion of the placenta. Here, we analysed the expression of key TSC transcription factors Cdx2, Eomes, and Elf5 in the early developing placenta of mouse embryos and in cultured TSCs and reveal surprising heterogeneity in protein levels. We analysed persistence of TSCs in the early placenta and find that TSCs remain in the chorionic hinge until E9.5 and are lost shortly afterwards. To define the transcriptional signature of bona fide TSCs, we used inducible gain- and loss-of-function alleles of Eomes or Cdx2, and EomesGFP, to manipulate and monitor the core maintenance factors of TSCs, followed by genome-wide expression profiling. Combinatorial analysis of resulting expression profiles allowed for defining novel TSC marker genes that might functionally contribute to the maintenance of the TSC state. Analyses by qRT-PCR and in situ hybridisation validated novel TSC- and chorion-specific marker genes, such as Bok/Mtd, Cldn26, Duox2, Duoxa2, Nr0b1, and Sox21. Thus, these expression data provide a valuable resource for the transcriptional signature of bona fide and early differentiating TSCs and may contribute to an increased understanding of the transcriptional circuitries that maintain and/or establish stemness of TSCs. |
| format | Article |
| id | doaj-art-0274a7673690412ea000793d938470d7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0274a7673690412ea000793d938470d72025-02-03T01:00:54ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/218518218518A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic PersistenceGeorg Kuales0Matthias Weiss1Oliver Sedelmeier2Dietmar Pfeifer3Sebastian J. Arnold4Renal Department, Centre for Clinical Research, University Medical Centre, Breisacher Strasse 66, 79106 Freiburg, GermanyRenal Department, Centre for Clinical Research, University Medical Centre, Breisacher Strasse 66, 79106 Freiburg, GermanyRenal Department, Centre for Clinical Research, University Medical Centre, Breisacher Strasse 66, 79106 Freiburg, GermanyDepartment of Hematology, Oncology and Stem Cell Transplantation, University Medical Centre, Freiburg, GermanyRenal Department, Centre for Clinical Research, University Medical Centre, Breisacher Strasse 66, 79106 Freiburg, GermanyTrophoblast stem cells (TSCs) represent the multipotent progenitors that give rise to the different cells of the embryonic portion of the placenta. Here, we analysed the expression of key TSC transcription factors Cdx2, Eomes, and Elf5 in the early developing placenta of mouse embryos and in cultured TSCs and reveal surprising heterogeneity in protein levels. We analysed persistence of TSCs in the early placenta and find that TSCs remain in the chorionic hinge until E9.5 and are lost shortly afterwards. To define the transcriptional signature of bona fide TSCs, we used inducible gain- and loss-of-function alleles of Eomes or Cdx2, and EomesGFP, to manipulate and monitor the core maintenance factors of TSCs, followed by genome-wide expression profiling. Combinatorial analysis of resulting expression profiles allowed for defining novel TSC marker genes that might functionally contribute to the maintenance of the TSC state. Analyses by qRT-PCR and in situ hybridisation validated novel TSC- and chorion-specific marker genes, such as Bok/Mtd, Cldn26, Duox2, Duoxa2, Nr0b1, and Sox21. Thus, these expression data provide a valuable resource for the transcriptional signature of bona fide and early differentiating TSCs and may contribute to an increased understanding of the transcriptional circuitries that maintain and/or establish stemness of TSCs.http://dx.doi.org/10.1155/2015/218518 |
| spellingShingle | Georg Kuales Matthias Weiss Oliver Sedelmeier Dietmar Pfeifer Sebastian J. Arnold A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic Persistence Stem Cells International |
| title | A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic Persistence |
| title_full | A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic Persistence |
| title_fullStr | A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic Persistence |
| title_full_unstemmed | A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic Persistence |
| title_short | A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic Persistence |
| title_sort | resource for the transcriptional signature of bona fide trophoblast stem cells and analysis of their embryonic persistence |
| url | http://dx.doi.org/10.1155/2015/218518 |
| work_keys_str_mv | AT georgkuales aresourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence AT matthiasweiss aresourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence AT oliversedelmeier aresourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence AT dietmarpfeifer aresourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence AT sebastianjarnold aresourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence AT georgkuales resourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence AT matthiasweiss resourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence AT oliversedelmeier resourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence AT dietmarpfeifer resourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence AT sebastianjarnold resourceforthetranscriptionalsignatureofbonafidetrophoblaststemcellsandanalysisoftheirembryonicpersistence |