Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, China
Salmonellosis, caused by non-typhoidal Salmonella, is a common foodborne gastrointestinal infection. Third-generation cephalosporins are recommended as the first-line treatment for Salmonella infections. Our study aimed to investigate the molecular epidemiology, antimicrobial resistance, and the tra...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1544757/full |
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| author | Jing Wang Jing Wang Zi-Han Dong Xian-Yuan Zhou Qin-Chun Ma Zhen-Yu Wang Dachuan Lin Ying-Feng Huang Chi Zhang Xinan Jiao Deng Li Qiuchun Li |
| author_facet | Jing Wang Jing Wang Zi-Han Dong Xian-Yuan Zhou Qin-Chun Ma Zhen-Yu Wang Dachuan Lin Ying-Feng Huang Chi Zhang Xinan Jiao Deng Li Qiuchun Li |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Salmonellosis, caused by non-typhoidal Salmonella, is a common foodborne gastrointestinal infection. Third-generation cephalosporins are recommended as the first-line treatment for Salmonella infections. Our study aimed to investigate the molecular epidemiology, antimicrobial resistance, and the transmission of extended-spectrum β-lactamases (ESBL) genes in 96 clinical Salmonella isolates collected between 2020 and 2022 at a tertiary hospital in Shenzhen, China. We performed antimicrobial susceptibility testing and whole-genome sequencing to identify serotypes, multilocus sequence typing, antimicrobial resistance genes in these isolates, and the genetic structures of the blaCTX-M/blaCMY genes. Seventeen Salmonella serotypes were identified, with S. 4,[5],12:i:- (37.5%) being the most common, followed by S. Enteritidis (15.63%), S. Typhimurium (14.58%), S. London (7.29%), and S. Rissen (5.21%). MLST analysis revealed 19 distinct sequence types (STs), with ST34 being the most prevalent (36.46%), followed by ST11 (15.63%) and ST19 (13.54%). Antimicrobial resistance testing showed those isolates had high levels of resistance to ampicillin (72.92%) and tetracycline (71.88%), with 70.83% of isolates as multidrug-resistant (MDR). Three blaCTX-M genes (blaCTX-M-14,blaCTX-M-55, and blaCTX-M-65) and blaCMY-2 were identified among 18 cefotaxime-resistant strains, of which one and 12 isolates successfully transferred blaCMY or blaCTX-M to E. coli C600 via conjugation, respectively. The blaCTX-M/blaCMY-2-carrying contigs in nine Salmonella isolates ranged from 2,156 to 164,862 bp, were located either on the chromosome (n=1) or plasmids (IncI1, IncK1, IncA/C) (n=9), and the blaCTX-M/blaCMY-2 genes were associated with ISEcp1. Our study demonstrates the diversity of MDR Salmonella serotypes in clinical isolates, and highlights the role of plasmids and mobile genetic elements in the horizontal transfer of blaCTX-M/blaCMY, emphasizing the need for continuous surveillance of Salmonella in clinical samples. |
| format | Article |
| id | doaj-art-026f1b2c68454e00a4c8197e044aca6b |
| institution | OA Journals |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-026f1b2c68454e00a4c8197e044aca6b2025-08-20T02:04:52ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-03-011510.3389/fcimb.2025.15447571544757Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, ChinaJing Wang0Jing Wang1Zi-Han Dong2Xian-Yuan Zhou3Qin-Chun Ma4Zhen-Yu Wang5Dachuan Lin6Ying-Feng Huang7Chi Zhang8Xinan Jiao9Deng Li10Qiuchun Li11Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaInstitute of Medical Sciences, School of Public Health, Xinjiang Medical University, Urumqi, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaDepartment of Laboratory Medicine, Shenzhen University General Hospital, Shenzhen, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Laboratory Medicine, Shenzhen University General Hospital, Shenzhen, ChinaDepartment of Laboratory Medicine, Shenzhen University General Hospital, Shenzhen, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaDepartment of Laboratory Medicine, Shenzhen University General Hospital, Shenzhen, ChinaJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaSalmonellosis, caused by non-typhoidal Salmonella, is a common foodborne gastrointestinal infection. Third-generation cephalosporins are recommended as the first-line treatment for Salmonella infections. Our study aimed to investigate the molecular epidemiology, antimicrobial resistance, and the transmission of extended-spectrum β-lactamases (ESBL) genes in 96 clinical Salmonella isolates collected between 2020 and 2022 at a tertiary hospital in Shenzhen, China. We performed antimicrobial susceptibility testing and whole-genome sequencing to identify serotypes, multilocus sequence typing, antimicrobial resistance genes in these isolates, and the genetic structures of the blaCTX-M/blaCMY genes. Seventeen Salmonella serotypes were identified, with S. 4,[5],12:i:- (37.5%) being the most common, followed by S. Enteritidis (15.63%), S. Typhimurium (14.58%), S. London (7.29%), and S. Rissen (5.21%). MLST analysis revealed 19 distinct sequence types (STs), with ST34 being the most prevalent (36.46%), followed by ST11 (15.63%) and ST19 (13.54%). Antimicrobial resistance testing showed those isolates had high levels of resistance to ampicillin (72.92%) and tetracycline (71.88%), with 70.83% of isolates as multidrug-resistant (MDR). Three blaCTX-M genes (blaCTX-M-14,blaCTX-M-55, and blaCTX-M-65) and blaCMY-2 were identified among 18 cefotaxime-resistant strains, of which one and 12 isolates successfully transferred blaCMY or blaCTX-M to E. coli C600 via conjugation, respectively. The blaCTX-M/blaCMY-2-carrying contigs in nine Salmonella isolates ranged from 2,156 to 164,862 bp, were located either on the chromosome (n=1) or plasmids (IncI1, IncK1, IncA/C) (n=9), and the blaCTX-M/blaCMY-2 genes were associated with ISEcp1. Our study demonstrates the diversity of MDR Salmonella serotypes in clinical isolates, and highlights the role of plasmids and mobile genetic elements in the horizontal transfer of blaCTX-M/blaCMY, emphasizing the need for continuous surveillance of Salmonella in clinical samples.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1544757/fullnon-typhoidal SalmonellaS. 4[5]12:i:-S. EnteritidisS. Typhimurium |
| spellingShingle | Jing Wang Jing Wang Zi-Han Dong Xian-Yuan Zhou Qin-Chun Ma Zhen-Yu Wang Dachuan Lin Ying-Feng Huang Chi Zhang Xinan Jiao Deng Li Qiuchun Li Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, China Frontiers in Cellular and Infection Microbiology non-typhoidal Salmonella S. 4 [5] 12:i:- S. Enteritidis S. Typhimurium |
| title | Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, China |
| title_full | Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, China |
| title_fullStr | Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, China |
| title_full_unstemmed | Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, China |
| title_short | Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, China |
| title_sort | stool carriage of ctx m cmy producing salmonella enterica in a chinese tertiary hospital in shenzhen china |
| topic | non-typhoidal Salmonella S. 4 [5] 12:i:- S. Enteritidis S. Typhimurium |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1544757/full |
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