Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer
Objective. Increased expression of KDM1A and decreased expression of DACT1 in cervical cancer cells were noticed in a previous study. This study is aimed at exploring the mechanism behind the KDM1A regulation on DACT1 in cervical cancer cells. Methods. The expression profile of KDM1A and DACT1 in ce...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2021/5555452 |
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| _version_ | 1832566039950393344 |
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| author | Lingjuan Zeng Chunyan Chen Chanjiao Yao |
| author_facet | Lingjuan Zeng Chunyan Chen Chanjiao Yao |
| author_sort | Lingjuan Zeng |
| collection | DOAJ |
| description | Objective. Increased expression of KDM1A and decreased expression of DACT1 in cervical cancer cells were noticed in a previous study. This study is aimed at exploring the mechanism behind the KDM1A regulation on DACT1 in cervical cancer cells. Methods. The expression profile of KDM1A and DACT1 in cervical cancer tissues was searched in TCGA database. In vitro experiments verified the effect of KDM1A and DACT1 on proliferation and migration ability of cervical cancer cell lines after cell transfection. The interaction of KDM1A with HDAC1 was identified by coimmunoprecipitation (Co-IP). The expression levels of KDM1A and DACT1 in cervical cancer cell lines were determined by qRT-PCR and western blot. Results. TCGA database showed that cervical cancer tissues had elevated expression of KDM1A and decreased expression of DACT1, which was consistent with the observation in cervical cancer cell lines. KDM1A was found to negatively regulate DACT1 through histone deacetylation. Meanwhile, the downregulation of KDM1A or overexpression of DACT1 could suppress the cell proliferation and migration ability in HeLa and SiHa cells. Cotransfection of KDM1A and DACT1 overexpression could reverse the increased cell proliferation and migration ability induced by KDM1A overexpression. Conclusion. KDM1A can downregulate DACT1 expression through histone deacetylation and therefore suppress the proliferation and migration of cervical cancer cells. |
| format | Article |
| id | doaj-art-025929af24914639a3b9fae461a3230f |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-025929af24914639a3b9fae461a3230f2025-02-03T01:05:12ZengWileyAnalytical Cellular Pathology2210-71772210-71852021-01-01202110.1155/2021/55554525555452Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical CancerLingjuan Zeng0Chunyan Chen1Chanjiao Yao2No. 3 Obstetrics and Gynecology Department, Hunan Provincial People’s Hospital, No. 61, West Jiefang Road, Furong District, Changsha, Hunan 410005, ChinaNo. 3 Obstetrics and Gynecology Department, Hunan Provincial People’s Hospital, No. 61, West Jiefang Road, Furong District, Changsha, Hunan 410005, ChinaNo. 3 Obstetrics and Gynecology Department, Hunan Provincial People’s Hospital, No. 61, West Jiefang Road, Furong District, Changsha, Hunan 410005, ChinaObjective. Increased expression of KDM1A and decreased expression of DACT1 in cervical cancer cells were noticed in a previous study. This study is aimed at exploring the mechanism behind the KDM1A regulation on DACT1 in cervical cancer cells. Methods. The expression profile of KDM1A and DACT1 in cervical cancer tissues was searched in TCGA database. In vitro experiments verified the effect of KDM1A and DACT1 on proliferation and migration ability of cervical cancer cell lines after cell transfection. The interaction of KDM1A with HDAC1 was identified by coimmunoprecipitation (Co-IP). The expression levels of KDM1A and DACT1 in cervical cancer cell lines were determined by qRT-PCR and western blot. Results. TCGA database showed that cervical cancer tissues had elevated expression of KDM1A and decreased expression of DACT1, which was consistent with the observation in cervical cancer cell lines. KDM1A was found to negatively regulate DACT1 through histone deacetylation. Meanwhile, the downregulation of KDM1A or overexpression of DACT1 could suppress the cell proliferation and migration ability in HeLa and SiHa cells. Cotransfection of KDM1A and DACT1 overexpression could reverse the increased cell proliferation and migration ability induced by KDM1A overexpression. Conclusion. KDM1A can downregulate DACT1 expression through histone deacetylation and therefore suppress the proliferation and migration of cervical cancer cells.http://dx.doi.org/10.1155/2021/5555452 |
| spellingShingle | Lingjuan Zeng Chunyan Chen Chanjiao Yao Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer Analytical Cellular Pathology |
| title | Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer |
| title_full | Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer |
| title_fullStr | Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer |
| title_full_unstemmed | Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer |
| title_short | Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer |
| title_sort | histone deacetylation regulated by kdm1a to suppress dact1 in proliferation and migration of cervical cancer |
| url | http://dx.doi.org/10.1155/2021/5555452 |
| work_keys_str_mv | AT lingjuanzeng histonedeacetylationregulatedbykdm1atosuppressdact1inproliferationandmigrationofcervicalcancer AT chunyanchen histonedeacetylationregulatedbykdm1atosuppressdact1inproliferationandmigrationofcervicalcancer AT chanjiaoyao histonedeacetylationregulatedbykdm1atosuppressdact1inproliferationandmigrationofcervicalcancer |