Omics insights into Bacillus velezensis LMY3-5 defense against Botryosphaeria dothidea in kiwifruit
Botryosphaeria dothidea is the main cause of soft rot in kiwifruit, significantly reducing both yield and quality. While chemical treatments are commonly used, their effectiveness is limited and they may pose environmental risks. As a result, biological control using Bacillus species has emerged as...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1633015/full |
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| author | Chunguang Ren Yu Liu Wenwen Su Zhengcheng Han Di Wu Weijie Li |
| author_facet | Chunguang Ren Yu Liu Wenwen Su Zhengcheng Han Di Wu Weijie Li |
| author_sort | Chunguang Ren |
| collection | DOAJ |
| description | Botryosphaeria dothidea is the main cause of soft rot in kiwifruit, significantly reducing both yield and quality. While chemical treatments are commonly used, their effectiveness is limited and they may pose environmental risks. As a result, biological control using Bacillus species has emerged as a promising alternative. In this study, we explored the antifungal mechanism of the biocontrol strain Bacillus velezensis LMY3-5 against kiwifruit soft rot through integrated genomic and transcriptomic analyses. In terms of antagonistic activity: B. velezensis LMY3-5 exhibits strong antagonism against B. dothidea, the causal agent of kiwifruit soft rot, showing a 70.44% biocontrol efficacy in inoculation tests. In terms of genomic features: genome 4.03 Mb circular chromosome with 46.5% GC content. Eight antimicrobial BGCs were identified, including those for surfactin, fengycin, bacillaene, bacillibactin, and others, explaining its biocontrol potential. In terms of transcriptomic insights: during coculture with B. dothidea, 114 DEGs (31 upregulated, 93 downregulated) were detected. Downregulated: flagellar assembly and chemotaxis genes (suggesting reduced motility during antagonism). Upregulated: genes linked to fengycin, siderophores, and lysozyme production, critical for antifungal activity. In terms of mechanism and implications lipopeptides (e.g., fengycin) and siderophores are key in inhibiting fungal growth. Findings support LMY3-5’s potential as a biocontrol agent for plant protection against B. dothidea. The main conclusion of this study is LMY3-5 combats B. dothidea via antimicrobial metabolites, with genomics and transcriptomics revealing its molecular basis for biocontrol. This strain holds promise for sustainable plant disease management. This may provide a theoretical basis for the potential application of B. velezensis LMY3-5 in the field of plant protection in the future. |
| format | Article |
| id | doaj-art-0247a6ba8dcd449ca2b10053473e9fb1 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-0247a6ba8dcd449ca2b10053473e9fb12025-08-20T05:32:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-08-011610.3389/fmicb.2025.16330151633015Omics insights into Bacillus velezensis LMY3-5 defense against Botryosphaeria dothidea in kiwifruitChunguang Ren0Yu Liu1Wenwen Su2Zhengcheng Han3Di Wu4Weijie Li5Guizhou Institute of Mountain Resources, Guiyang, ChinaGuizhou Institute of Mountain Resources, Guiyang, ChinaGuizhou Institute of Mountain Resources, Guiyang, ChinaGuizhou Botanical Garden, Guiyang, ChinaGuizhou Institute of Mountain Resources, Guiyang, ChinaGuizhou Botanical Garden, Guiyang, ChinaBotryosphaeria dothidea is the main cause of soft rot in kiwifruit, significantly reducing both yield and quality. While chemical treatments are commonly used, their effectiveness is limited and they may pose environmental risks. As a result, biological control using Bacillus species has emerged as a promising alternative. In this study, we explored the antifungal mechanism of the biocontrol strain Bacillus velezensis LMY3-5 against kiwifruit soft rot through integrated genomic and transcriptomic analyses. In terms of antagonistic activity: B. velezensis LMY3-5 exhibits strong antagonism against B. dothidea, the causal agent of kiwifruit soft rot, showing a 70.44% biocontrol efficacy in inoculation tests. In terms of genomic features: genome 4.03 Mb circular chromosome with 46.5% GC content. Eight antimicrobial BGCs were identified, including those for surfactin, fengycin, bacillaene, bacillibactin, and others, explaining its biocontrol potential. In terms of transcriptomic insights: during coculture with B. dothidea, 114 DEGs (31 upregulated, 93 downregulated) were detected. Downregulated: flagellar assembly and chemotaxis genes (suggesting reduced motility during antagonism). Upregulated: genes linked to fengycin, siderophores, and lysozyme production, critical for antifungal activity. In terms of mechanism and implications lipopeptides (e.g., fengycin) and siderophores are key in inhibiting fungal growth. Findings support LMY3-5’s potential as a biocontrol agent for plant protection against B. dothidea. The main conclusion of this study is LMY3-5 combats B. dothidea via antimicrobial metabolites, with genomics and transcriptomics revealing its molecular basis for biocontrol. This strain holds promise for sustainable plant disease management. This may provide a theoretical basis for the potential application of B. velezensis LMY3-5 in the field of plant protection in the future.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1633015/fullBacillus velezensisBotryosphaeria dothideakiwifruit soft rotbiocontrolantimicrobial mechanisms |
| spellingShingle | Chunguang Ren Yu Liu Wenwen Su Zhengcheng Han Di Wu Weijie Li Omics insights into Bacillus velezensis LMY3-5 defense against Botryosphaeria dothidea in kiwifruit Frontiers in Microbiology Bacillus velezensis Botryosphaeria dothidea kiwifruit soft rot biocontrol antimicrobial mechanisms |
| title | Omics insights into Bacillus velezensis LMY3-5 defense against Botryosphaeria dothidea in kiwifruit |
| title_full | Omics insights into Bacillus velezensis LMY3-5 defense against Botryosphaeria dothidea in kiwifruit |
| title_fullStr | Omics insights into Bacillus velezensis LMY3-5 defense against Botryosphaeria dothidea in kiwifruit |
| title_full_unstemmed | Omics insights into Bacillus velezensis LMY3-5 defense against Botryosphaeria dothidea in kiwifruit |
| title_short | Omics insights into Bacillus velezensis LMY3-5 defense against Botryosphaeria dothidea in kiwifruit |
| title_sort | omics insights into bacillus velezensis lmy3 5 defense against botryosphaeria dothidea in kiwifruit |
| topic | Bacillus velezensis Botryosphaeria dothidea kiwifruit soft rot biocontrol antimicrobial mechanisms |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1633015/full |
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