Unraveling the immunomodulatory and metabolic effects of bioactive glass S53P4 on macrophages in vitro
Abstract Macrophage metabolism is closely linked to their phenotype and function, which is why there is growing interest in studying the metabolic reprogramming of macrophages. Bioactive glass (BG) S53P4 is a bioactive material used especially in bone applications. Additionally, BG S53P4 has been sh...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2025-01-01
|
| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-025-06861-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832586030975287296 |
|---|---|
| author | Karoliina Kajander Nicole Nowak Negin Vaziri Pekka K. Vallittu Terhi J. Heino Jorma A. Määttä |
| author_facet | Karoliina Kajander Nicole Nowak Negin Vaziri Pekka K. Vallittu Terhi J. Heino Jorma A. Määttä |
| author_sort | Karoliina Kajander |
| collection | DOAJ |
| description | Abstract Macrophage metabolism is closely linked to their phenotype and function, which is why there is growing interest in studying the metabolic reprogramming of macrophages. Bioactive glass (BG) S53P4 is a bioactive material used especially in bone applications. Additionally, BG S53P4 has been shown to affect macrophages, but the mechanisms through which the possible immunomodulatory effects are conveyed remain unclear. According to the results presented here, the lipopolysaccharide (LPS) induced suppression in oxidative phosphorylation is rescued in macrophages cultured with BG S53P4 before the inflammatory stimulus. Additionally, BG S53P4-exposed macrophages expressed lower mRNA levels of inflammatory cytokines Il6 and Il1b, as well as demonstrated decreased activation of inflammatory interferon regulatory factor (IRF) and NF-κB pathways and nitrogen oxide secretion in response to LPS. These results did not rely on cells being in direct contact with the material as similar effects were observed in the presence of BG S53P4-conditioned medium. Our findings link the immunomodulatory properties of BG S53P4 and macrophage metabolism, which improves our understanding of the mechanisms underlying the clinical efficacy of bioactive glasses. Graphical Abstract |
| format | Article |
| id | doaj-art-0217b87a1c8143bb9becac13521fc0ce |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-0217b87a1c8143bb9becac13521fc0ce2025-01-26T12:12:50ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382025-01-0136111510.1007/s10856-025-06861-yUnraveling the immunomodulatory and metabolic effects of bioactive glass S53P4 on macrophages in vitroKaroliina Kajander0Nicole Nowak1Negin Vaziri2Pekka K. Vallittu3Terhi J. Heino4Jorma A. Määttä5Institute of Biomedicine, Faculty of Medicine, University of TurkuInstitute of Biomedicine, Faculty of Medicine, University of TurkuInstitute of Biomedicine, Faculty of Medicine, University of TurkuDepartment of Biomaterials Science and Turku Clinical Biomaterials Centre – TCBC, Institute of Dentistry, University of TurkuInstitute of Biomedicine, Faculty of Medicine, University of TurkuInstitute of Biomedicine, Faculty of Medicine, University of TurkuAbstract Macrophage metabolism is closely linked to their phenotype and function, which is why there is growing interest in studying the metabolic reprogramming of macrophages. Bioactive glass (BG) S53P4 is a bioactive material used especially in bone applications. Additionally, BG S53P4 has been shown to affect macrophages, but the mechanisms through which the possible immunomodulatory effects are conveyed remain unclear. According to the results presented here, the lipopolysaccharide (LPS) induced suppression in oxidative phosphorylation is rescued in macrophages cultured with BG S53P4 before the inflammatory stimulus. Additionally, BG S53P4-exposed macrophages expressed lower mRNA levels of inflammatory cytokines Il6 and Il1b, as well as demonstrated decreased activation of inflammatory interferon regulatory factor (IRF) and NF-κB pathways and nitrogen oxide secretion in response to LPS. These results did not rely on cells being in direct contact with the material as similar effects were observed in the presence of BG S53P4-conditioned medium. Our findings link the immunomodulatory properties of BG S53P4 and macrophage metabolism, which improves our understanding of the mechanisms underlying the clinical efficacy of bioactive glasses. Graphical Abstracthttps://doi.org/10.1007/s10856-025-06861-y |
| spellingShingle | Karoliina Kajander Nicole Nowak Negin Vaziri Pekka K. Vallittu Terhi J. Heino Jorma A. Määttä Unraveling the immunomodulatory and metabolic effects of bioactive glass S53P4 on macrophages in vitro Journal of Materials Science: Materials in Medicine |
| title | Unraveling the immunomodulatory and metabolic effects of bioactive glass S53P4 on macrophages in vitro |
| title_full | Unraveling the immunomodulatory and metabolic effects of bioactive glass S53P4 on macrophages in vitro |
| title_fullStr | Unraveling the immunomodulatory and metabolic effects of bioactive glass S53P4 on macrophages in vitro |
| title_full_unstemmed | Unraveling the immunomodulatory and metabolic effects of bioactive glass S53P4 on macrophages in vitro |
| title_short | Unraveling the immunomodulatory and metabolic effects of bioactive glass S53P4 on macrophages in vitro |
| title_sort | unraveling the immunomodulatory and metabolic effects of bioactive glass s53p4 on macrophages in vitro |
| url | https://doi.org/10.1007/s10856-025-06861-y |
| work_keys_str_mv | AT karoliinakajander unravelingtheimmunomodulatoryandmetaboliceffectsofbioactiveglasss53p4onmacrophagesinvitro AT nicolenowak unravelingtheimmunomodulatoryandmetaboliceffectsofbioactiveglasss53p4onmacrophagesinvitro AT neginvaziri unravelingtheimmunomodulatoryandmetaboliceffectsofbioactiveglasss53p4onmacrophagesinvitro AT pekkakvallittu unravelingtheimmunomodulatoryandmetaboliceffectsofbioactiveglasss53p4onmacrophagesinvitro AT terhijheino unravelingtheimmunomodulatoryandmetaboliceffectsofbioactiveglasss53p4onmacrophagesinvitro AT jormaamaatta unravelingtheimmunomodulatoryandmetaboliceffectsofbioactiveglasss53p4onmacrophagesinvitro |