Highly Sensitive Colorimetric Assay for Determining Fe3+ Based on Gold Nanoparticles Conjugated with Glycol Chitosan
A highly sensitive and simple colorimetric assay for the detection of Fe3+ ions was developed using gold nanoparticles (AuNPs) conjugated with glycol chitosan (GC). The Fe3+ ion coordinates with the oxygen atoms of GC in a hexadentate manner (O-Fe3+-O), decreasing the interparticle distance and indu...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/3648564 |
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| author | Kyungmin Kim Yun-Sik Nam Yeonhee Lee Kang-Bong Lee |
| author_facet | Kyungmin Kim Yun-Sik Nam Yeonhee Lee Kang-Bong Lee |
| author_sort | Kyungmin Kim |
| collection | DOAJ |
| description | A highly sensitive and simple colorimetric assay for the detection of Fe3+ ions was developed using gold nanoparticles (AuNPs) conjugated with glycol chitosan (GC). The Fe3+ ion coordinates with the oxygen atoms of GC in a hexadentate manner (O-Fe3+-O), decreasing the interparticle distance and inducing aggregation. Time-of-flight secondary ion mass spectrometry showed that the bound Fe3+ was coordinated to the oxygen atoms of the ethylene glycol in GC, which resulted in a significant color change from light red to dark midnight blue due to aggregation. Using this GC-AuNP probe, the quantitative determination of Fe3+ in biological, environmental, and pharmaceutical samples could be achieved by the naked eye and spectrophotometric methods. Sensitive response and pronounced color change of the GC-AuNPs in the presence of Fe3+ were optimized at pH 6, 70°C, and 300 mM NaCl concentration. The absorption intensity ratio (A700/A510) linearly correlated to the Fe3+ concentration in the linear range of 0–180 μM. The limits of detection were 11.3, 29.2, and 46.0 nM for tap water, pond water, and iron supplement tablets, respectively. Owing to its facile and sensitive nature, this assay method for Fe3+ ions can be applied to the analysis of drinking water and pharmaceutical samples. |
| format | Article |
| id | doaj-art-02018e7ba55f40ef86341fd7092a62b5 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-02018e7ba55f40ef86341fd7092a62b52025-02-03T01:21:07ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/36485643648564Highly Sensitive Colorimetric Assay for Determining Fe3+ Based on Gold Nanoparticles Conjugated with Glycol ChitosanKyungmin Kim0Yun-Sik Nam1Yeonhee Lee2Kang-Bong Lee3Green City Technology Institute, Korea Institute of Science and Technology, Hwarang-ro 14gil 5, Seongbuk-gu, Seoul 02792, Republic of KoreaAdvanced Analysis Center, Korea Institute of Science and Technology, Hwarang-ro 14gil 5, Seongbuk-gu, Seoul 02792, Republic of KoreaAdvanced Analysis Center, Korea Institute of Science and Technology, Hwarang-ro 14gil 5, Seongbuk-gu, Seoul 02792, Republic of KoreaGreen City Technology Institute, Korea Institute of Science and Technology, Hwarang-ro 14gil 5, Seongbuk-gu, Seoul 02792, Republic of KoreaA highly sensitive and simple colorimetric assay for the detection of Fe3+ ions was developed using gold nanoparticles (AuNPs) conjugated with glycol chitosan (GC). The Fe3+ ion coordinates with the oxygen atoms of GC in a hexadentate manner (O-Fe3+-O), decreasing the interparticle distance and inducing aggregation. Time-of-flight secondary ion mass spectrometry showed that the bound Fe3+ was coordinated to the oxygen atoms of the ethylene glycol in GC, which resulted in a significant color change from light red to dark midnight blue due to aggregation. Using this GC-AuNP probe, the quantitative determination of Fe3+ in biological, environmental, and pharmaceutical samples could be achieved by the naked eye and spectrophotometric methods. Sensitive response and pronounced color change of the GC-AuNPs in the presence of Fe3+ were optimized at pH 6, 70°C, and 300 mM NaCl concentration. The absorption intensity ratio (A700/A510) linearly correlated to the Fe3+ concentration in the linear range of 0–180 μM. The limits of detection were 11.3, 29.2, and 46.0 nM for tap water, pond water, and iron supplement tablets, respectively. Owing to its facile and sensitive nature, this assay method for Fe3+ ions can be applied to the analysis of drinking water and pharmaceutical samples.http://dx.doi.org/10.1155/2017/3648564 |
| spellingShingle | Kyungmin Kim Yun-Sik Nam Yeonhee Lee Kang-Bong Lee Highly Sensitive Colorimetric Assay for Determining Fe3+ Based on Gold Nanoparticles Conjugated with Glycol Chitosan Journal of Analytical Methods in Chemistry |
| title | Highly Sensitive Colorimetric Assay for Determining Fe3+ Based on Gold Nanoparticles Conjugated with Glycol Chitosan |
| title_full | Highly Sensitive Colorimetric Assay for Determining Fe3+ Based on Gold Nanoparticles Conjugated with Glycol Chitosan |
| title_fullStr | Highly Sensitive Colorimetric Assay for Determining Fe3+ Based on Gold Nanoparticles Conjugated with Glycol Chitosan |
| title_full_unstemmed | Highly Sensitive Colorimetric Assay for Determining Fe3+ Based on Gold Nanoparticles Conjugated with Glycol Chitosan |
| title_short | Highly Sensitive Colorimetric Assay for Determining Fe3+ Based on Gold Nanoparticles Conjugated with Glycol Chitosan |
| title_sort | highly sensitive colorimetric assay for determining fe3 based on gold nanoparticles conjugated with glycol chitosan |
| url | http://dx.doi.org/10.1155/2017/3648564 |
| work_keys_str_mv | AT kyungminkim highlysensitivecolorimetricassayfordeterminingfe3basedongoldnanoparticlesconjugatedwithglycolchitosan AT yunsiknam highlysensitivecolorimetricassayfordeterminingfe3basedongoldnanoparticlesconjugatedwithglycolchitosan AT yeonheelee highlysensitivecolorimetricassayfordeterminingfe3basedongoldnanoparticlesconjugatedwithglycolchitosan AT kangbonglee highlysensitivecolorimetricassayfordeterminingfe3basedongoldnanoparticlesconjugatedwithglycolchitosan |