A GP130-Targeting Small Molecule, LMT-28, Reduces LPS-Induced Bone Resorption around Implants in Diabetic Models by Inhibiting IL-6/GP130/JAK2/STAT3 Signaling
In this study, we examined the effect of the GP130-targeting molecule, LMT-28, on lipopolysaccharide- (LPS-) induced bone resorption around implants in diabetic models using in vitro and rat animal experiments. First, LMT-28 was added to osteoblasts stimulated by LPS and advanced glycation end produ...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2023/9330439 |
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| author | Qi-qi Liu Wei-wei Wu Jian Yang Rui-bin Wang Ling-ling Yuan Pei-zhao Peng Mao-yun Zeng Ke Yu |
| author_facet | Qi-qi Liu Wei-wei Wu Jian Yang Rui-bin Wang Ling-ling Yuan Pei-zhao Peng Mao-yun Zeng Ke Yu |
| author_sort | Qi-qi Liu |
| collection | DOAJ |
| description | In this study, we examined the effect of the GP130-targeting molecule, LMT-28, on lipopolysaccharide- (LPS-) induced bone resorption around implants in diabetic models using in vitro and rat animal experiments. First, LMT-28 was added to osteoblasts stimulated by LPS and advanced glycation end products (AGEs), and nuclear factor-κB receptor-activating factor ligand (RANKL) and associated pathways were evaluated. Then, LMT-28 was administered by gavage at 0.23 mg/kg once every 5 days for 2 weeks to type 2 diabetic rats with peri-implantitis induced by LPS injection and silk ligature. The expression of IL-6 and RANKL was evaluated by immunohistochemistry, and the bone resorption around implants was evaluated by microcomputed tomography. The results showed that LMT-28 downregulated the expression of RANKL through the JAK2/STAT3 signaling pathway in osteoblasts stimulated by LPS and AGEs, reduced bone resorption around implants with peri-implantitis, decreased the expression of IL-6 and RANKL, and decreased osteoclast activity in type 2 diabetic rats. This study confirmed the ability of LMT-28 to reduce LPS-induced bone resorption around implants in diabetic rats. |
| format | Article |
| id | doaj-art-01cfe5bd5507465aad1be8dcdb18a0b0 |
| institution | Kabale University |
| issn | 1466-1861 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-01cfe5bd5507465aad1be8dcdb18a0b02025-02-03T05:57:24ZengWileyMediators of Inflammation1466-18612023-01-01202310.1155/2023/9330439A GP130-Targeting Small Molecule, LMT-28, Reduces LPS-Induced Bone Resorption around Implants in Diabetic Models by Inhibiting IL-6/GP130/JAK2/STAT3 SignalingQi-qi Liu0Wei-wei Wu1Jian Yang2Rui-bin Wang3Ling-ling Yuan4Pei-zhao Peng5Mao-yun Zeng6Ke Yu7The Affiliated Stomatological Hospital of Southwest Medical UniversityThe Affiliated Stomatological Hospital of Southwest Medical UniversityThe Affiliated Stomatological Hospital of Southwest Medical UniversityThe Affiliated Stomatological Hospital of Southwest Medical UniversityThe Affiliated Stomatological Hospital of Southwest Medical UniversityThe Affiliated Stomatological Hospital of Southwest Medical UniversityThe Affiliated Stomatological Hospital of Southwest Medical UniversityThe Affiliated Stomatological Hospital of Southwest Medical UniversityIn this study, we examined the effect of the GP130-targeting molecule, LMT-28, on lipopolysaccharide- (LPS-) induced bone resorption around implants in diabetic models using in vitro and rat animal experiments. First, LMT-28 was added to osteoblasts stimulated by LPS and advanced glycation end products (AGEs), and nuclear factor-κB receptor-activating factor ligand (RANKL) and associated pathways were evaluated. Then, LMT-28 was administered by gavage at 0.23 mg/kg once every 5 days for 2 weeks to type 2 diabetic rats with peri-implantitis induced by LPS injection and silk ligature. The expression of IL-6 and RANKL was evaluated by immunohistochemistry, and the bone resorption around implants was evaluated by microcomputed tomography. The results showed that LMT-28 downregulated the expression of RANKL through the JAK2/STAT3 signaling pathway in osteoblasts stimulated by LPS and AGEs, reduced bone resorption around implants with peri-implantitis, decreased the expression of IL-6 and RANKL, and decreased osteoclast activity in type 2 diabetic rats. This study confirmed the ability of LMT-28 to reduce LPS-induced bone resorption around implants in diabetic rats.http://dx.doi.org/10.1155/2023/9330439 |
| spellingShingle | Qi-qi Liu Wei-wei Wu Jian Yang Rui-bin Wang Ling-ling Yuan Pei-zhao Peng Mao-yun Zeng Ke Yu A GP130-Targeting Small Molecule, LMT-28, Reduces LPS-Induced Bone Resorption around Implants in Diabetic Models by Inhibiting IL-6/GP130/JAK2/STAT3 Signaling Mediators of Inflammation |
| title | A GP130-Targeting Small Molecule, LMT-28, Reduces LPS-Induced Bone Resorption around Implants in Diabetic Models by Inhibiting IL-6/GP130/JAK2/STAT3 Signaling |
| title_full | A GP130-Targeting Small Molecule, LMT-28, Reduces LPS-Induced Bone Resorption around Implants in Diabetic Models by Inhibiting IL-6/GP130/JAK2/STAT3 Signaling |
| title_fullStr | A GP130-Targeting Small Molecule, LMT-28, Reduces LPS-Induced Bone Resorption around Implants in Diabetic Models by Inhibiting IL-6/GP130/JAK2/STAT3 Signaling |
| title_full_unstemmed | A GP130-Targeting Small Molecule, LMT-28, Reduces LPS-Induced Bone Resorption around Implants in Diabetic Models by Inhibiting IL-6/GP130/JAK2/STAT3 Signaling |
| title_short | A GP130-Targeting Small Molecule, LMT-28, Reduces LPS-Induced Bone Resorption around Implants in Diabetic Models by Inhibiting IL-6/GP130/JAK2/STAT3 Signaling |
| title_sort | gp130 targeting small molecule lmt 28 reduces lps induced bone resorption around implants in diabetic models by inhibiting il 6 gp130 jak2 stat3 signaling |
| url | http://dx.doi.org/10.1155/2023/9330439 |
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