Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation
One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to i...
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Polish Academy of Sciences Committee of Chemical and Process Engineering
2017-06-01
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| Series: | Chemical and Process Engineering |
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| Online Access: | http://www.degruyter.com/view/j/cpe.2017.38.issue-2/cpe-2017-0025/cpe-2017-0025.xml?format=INT |
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| author | Pilarek Maciej Godlewska Klaudia Kuźmińska Aleksandra Wojasiński Michał Dąbkowska Katarzyna |
| author_facet | Pilarek Maciej Godlewska Klaudia Kuźmińska Aleksandra Wojasiński Michał Dąbkowska Katarzyna |
| author_sort | Pilarek Maciej |
| collection | DOAJ |
| description | One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously wave-induced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices. |
| format | Article |
| id | doaj-art-01ba8e57597c4d40b66a6dba54cfc1bb |
| institution | OA Journals |
| issn | 2300-1925 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Polish Academy of Sciences Committee of Chemical and Process Engineering |
| record_format | Article |
| series | Chemical and Process Engineering |
| spelling | doaj-art-01ba8e57597c4d40b66a6dba54cfc1bb2025-08-20T02:25:48ZengPolish Academy of Sciences Committee of Chemical and Process EngineeringChemical and Process Engineering2300-19252017-06-0138232133010.1515/cpe-2017-0025cpe-2017-0025Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced AgitationPilarek Maciej0Godlewska Klaudia1Kuźmińska Aleksandra2Wojasiński Michał3Dąbkowska Katarzyna4Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Warsaw, PolandWarsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Warsaw, PolandWarsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Warsaw, PolandWarsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Warsaw, PolandWarsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Warsaw, PolandOne of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously wave-induced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.http://www.degruyter.com/view/j/cpe.2017.38.issue-2/cpe-2017-0025/cpe-2017-0025.xml?format=INTwave-induced agitationsmall-scale animal cell cultureCP5 chondrocytesfibrousbased scaffoldsingle-use bioreactor |
| spellingShingle | Pilarek Maciej Godlewska Klaudia Kuźmińska Aleksandra Wojasiński Michał Dąbkowska Katarzyna Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation Chemical and Process Engineering wave-induced agitation small-scale animal cell culture CP5 chondrocytes fibrousbased scaffold single-use bioreactor |
| title | Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation |
| title_full | Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation |
| title_fullStr | Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation |
| title_full_unstemmed | Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation |
| title_short | Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation |
| title_sort | enhanced chondrocyte proliferation in a prototyped culture system with wave induced agitation |
| topic | wave-induced agitation small-scale animal cell culture CP5 chondrocytes fibrousbased scaffold single-use bioreactor |
| url | http://www.degruyter.com/view/j/cpe.2017.38.issue-2/cpe-2017-0025/cpe-2017-0025.xml?format=INT |
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