Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent
Here, we aimed to answer important and fundamental questions in germ cell biology with special focus on the age of the male donor cells and the possibility to generate embryonic stem cell- (ESC-) like cells. While it is believed that spermatogonial stem cells (SSCs) and truly pluripotent ESC-like ce...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/8216312 |
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| author | Hossein Azizi Sabine Conrad Ursula Hinz Behrouz Asgari Daniel Nanus Heike Peterziel Akbar Hajizadeh Moghaddam Hossein Baharvand Thomas Skutella |
| author_facet | Hossein Azizi Sabine Conrad Ursula Hinz Behrouz Asgari Daniel Nanus Heike Peterziel Akbar Hajizadeh Moghaddam Hossein Baharvand Thomas Skutella |
| author_sort | Hossein Azizi |
| collection | DOAJ |
| description | Here, we aimed to answer important and fundamental questions in germ cell biology with special focus on the age of the male donor cells and the possibility to generate embryonic stem cell- (ESC-) like cells. While it is believed that spermatogonial stem cells (SSCs) and truly pluripotent ESC-like cells can be isolated from adult mice, it remained unknown if the spontaneous conversion of SSCs to ESC-like cells fails at some age. Similarly, there have been differences in the literature about the duration of cultures during which ESC-like cells may appear. We demonstrate the possibility to derive ESC-like cells from SSC cultures until they reach adolescence or up to 7 weeks of age, but we point out the impossibility to derive these cells from older, mature adult mice. The inability of real adult SSCs to shift to a pluripotent state coincides with a decline in expression of the core pluripotency genes Oct4, Nanog, and Sox2 in SSCs with age. At the same time genes of the spermatogonial differentiation pathway increase. The generated ESC-like cells were similar to ESCs and express pluripotency markers. In vitro they differentiate into all three germ lineages; they form complex teratomas after transplantation in SCID mice and produce chimeric mice. |
| format | Article |
| id | doaj-art-019f372d7788444d889d476bdc73fffa |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-019f372d7788444d889d476bdc73fffa2025-02-03T01:25:53ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/82163128216312Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age DependentHossein Azizi0Sabine Conrad1Ursula Hinz2Behrouz Asgari3Daniel Nanus4Heike Peterziel5Akbar Hajizadeh Moghaddam6Hossein Baharvand7Thomas Skutella8Institute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, GermanyP.O. Box 12 43, 72072 Tübingen, GermanyInstitute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, GermanyDepartment of Stem Cells and Developmental Biology at the Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, P.O. Box 19395, Tehran 4644, IranInstitute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, GermanyDivision of Signal Transduction and Growth Control, DKFZ/ZMBH Alliance, Heidelberg, GermanyDepartment of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, IranDepartment of Stem Cells and Developmental Biology at the Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, P.O. Box 19395, Tehran 4644, IranInstitute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, GermanyHere, we aimed to answer important and fundamental questions in germ cell biology with special focus on the age of the male donor cells and the possibility to generate embryonic stem cell- (ESC-) like cells. While it is believed that spermatogonial stem cells (SSCs) and truly pluripotent ESC-like cells can be isolated from adult mice, it remained unknown if the spontaneous conversion of SSCs to ESC-like cells fails at some age. Similarly, there have been differences in the literature about the duration of cultures during which ESC-like cells may appear. We demonstrate the possibility to derive ESC-like cells from SSC cultures until they reach adolescence or up to 7 weeks of age, but we point out the impossibility to derive these cells from older, mature adult mice. The inability of real adult SSCs to shift to a pluripotent state coincides with a decline in expression of the core pluripotency genes Oct4, Nanog, and Sox2 in SSCs with age. At the same time genes of the spermatogonial differentiation pathway increase. The generated ESC-like cells were similar to ESCs and express pluripotency markers. In vitro they differentiate into all three germ lineages; they form complex teratomas after transplantation in SCID mice and produce chimeric mice.http://dx.doi.org/10.1155/2016/8216312 |
| spellingShingle | Hossein Azizi Sabine Conrad Ursula Hinz Behrouz Asgari Daniel Nanus Heike Peterziel Akbar Hajizadeh Moghaddam Hossein Baharvand Thomas Skutella Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent Stem Cells International |
| title | Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent |
| title_full | Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent |
| title_fullStr | Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent |
| title_full_unstemmed | Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent |
| title_short | Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent |
| title_sort | derivation of pluripotent cells from mouse sscs seems to be age dependent |
| url | http://dx.doi.org/10.1155/2016/8216312 |
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