Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells
Early repair of skin injury and maximal restoration of the function and appearance have become important targets of clinical treatment. In the present study, we observed the healing process of skin defects in nude mice and structural characteristics of the new skin after transplantation of isolated...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/8281235 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554472918745088 |
|---|---|
| author | Wu Minjuan Xiong Jun Shao Shiyun Xu Sha Ni Haitao Wang Yue Ji Kaihong |
| author_facet | Wu Minjuan Xiong Jun Shao Shiyun Xu Sha Ni Haitao Wang Yue Ji Kaihong |
| author_sort | Wu Minjuan |
| collection | DOAJ |
| description | Early repair of skin injury and maximal restoration of the function and appearance have become important targets of clinical treatment. In the present study, we observed the healing process of skin defects in nude mice and structural characteristics of the new skin after transplantation of isolated and cultured adipose derived mesenchymal stem cells (ADMSCs) onto the human acellular amniotic membrane (AAM). The result showed that ADMSCs were closely attached to the surface of AAM and grew well 24 h after seeding. Comparison of the wound healing rate at days 7, 14, and 28 after transplantation showed that ADMSCs seeded on AAM facilitated the healing of full-thickness skin wounds more effectively as compared with either hAM or AAM alone, indicating that ADMSCs participated in skin regeneration. More importantly, we noticed a phenomenon of hair follicle development during the process of skin repair. Composite ADMSCs and AAM not only promoted the healing of the mouse full-thickness defects but also facilitated generation of the appendages of the affected skin, thus promoting restoration of the skin function. Our results provide a new possible therapy idea for the treatment of skin wounds with respect to both anatomical regeneration and functional restoration. |
| format | Article |
| id | doaj-art-01904aa528b74f71a6c70255fe8cc9da |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-01904aa528b74f71a6c70255fe8cc9da2025-02-03T05:51:22ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/82812358281235Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem CellsWu Minjuan0Xiong Jun1Shao Shiyun2Xu Sha3Ni Haitao4Wang Yue5Ji Kaihong6Department of Histology and Embryology, Second Military Medical University, Shanghai 200433, ChinaDepartment of Histology and Embryology, Second Military Medical University, Shanghai 200433, ChinaDepartment of Histology and Embryology, Second Military Medical University, Shanghai 200433, ChinaDepartment of Histology and Embryology, Second Military Medical University, Shanghai 200433, ChinaDepartment of Histology and Embryology, Second Military Medical University, Shanghai 200433, ChinaDepartment of Histology and Embryology, Second Military Medical University, Shanghai 200433, ChinaDepartment of Histology and Embryology, Second Military Medical University, Shanghai 200433, ChinaEarly repair of skin injury and maximal restoration of the function and appearance have become important targets of clinical treatment. In the present study, we observed the healing process of skin defects in nude mice and structural characteristics of the new skin after transplantation of isolated and cultured adipose derived mesenchymal stem cells (ADMSCs) onto the human acellular amniotic membrane (AAM). The result showed that ADMSCs were closely attached to the surface of AAM and grew well 24 h after seeding. Comparison of the wound healing rate at days 7, 14, and 28 after transplantation showed that ADMSCs seeded on AAM facilitated the healing of full-thickness skin wounds more effectively as compared with either hAM or AAM alone, indicating that ADMSCs participated in skin regeneration. More importantly, we noticed a phenomenon of hair follicle development during the process of skin repair. Composite ADMSCs and AAM not only promoted the healing of the mouse full-thickness defects but also facilitated generation of the appendages of the affected skin, thus promoting restoration of the skin function. Our results provide a new possible therapy idea for the treatment of skin wounds with respect to both anatomical regeneration and functional restoration.http://dx.doi.org/10.1155/2016/8281235 |
| spellingShingle | Wu Minjuan Xiong Jun Shao Shiyun Xu Sha Ni Haitao Wang Yue Ji Kaihong Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells Stem Cells International |
| title | Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells |
| title_full | Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells |
| title_fullStr | Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells |
| title_short | Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells |
| title_sort | hair follicle morphogenesis in the treatment of mouse full thickness skin defects using composite human acellular amniotic membrane and adipose derived mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2016/8281235 |
| work_keys_str_mv | AT wuminjuan hairfolliclemorphogenesisinthetreatmentofmousefullthicknessskindefectsusingcompositehumanacellularamnioticmembraneandadiposederivedmesenchymalstemcells AT xiongjun hairfolliclemorphogenesisinthetreatmentofmousefullthicknessskindefectsusingcompositehumanacellularamnioticmembraneandadiposederivedmesenchymalstemcells AT shaoshiyun hairfolliclemorphogenesisinthetreatmentofmousefullthicknessskindefectsusingcompositehumanacellularamnioticmembraneandadiposederivedmesenchymalstemcells AT xusha hairfolliclemorphogenesisinthetreatmentofmousefullthicknessskindefectsusingcompositehumanacellularamnioticmembraneandadiposederivedmesenchymalstemcells AT nihaitao hairfolliclemorphogenesisinthetreatmentofmousefullthicknessskindefectsusingcompositehumanacellularamnioticmembraneandadiposederivedmesenchymalstemcells AT wangyue hairfolliclemorphogenesisinthetreatmentofmousefullthicknessskindefectsusingcompositehumanacellularamnioticmembraneandadiposederivedmesenchymalstemcells AT jikaihong hairfolliclemorphogenesisinthetreatmentofmousefullthicknessskindefectsusingcompositehumanacellularamnioticmembraneandadiposederivedmesenchymalstemcells |