A Metabolic Study on Colon Cancer Using 1H Nuclear Magnetic Resonance Spectroscopy
Background. Colorectal carcinoma is the third cause of cancer deaths in the world. For diagnosis, invasive methods like colonoscopy and sigmoidoscopy are used, and noninvasive screening tests are not very accurate. We decided to study the potential of 1HNMR spectroscopy with metabolomics and chemom...
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Wiley
2014-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2014/348712 |
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| author | Zahra Zamani Mohammad Arjmand Farideh Vahabi Seyed Mahmood Eshaq Hosseini Sadegh Mohammad Fazeli Ayda Iravani Parastoo Bayat Akbar Oghalayee Mahshid Mehrabanfar Reza Haj Hosseini Mohammad Tashakorpour Mohsen Tafazzoli Sedigheh Sadeghi |
| author_facet | Zahra Zamani Mohammad Arjmand Farideh Vahabi Seyed Mahmood Eshaq Hosseini Sadegh Mohammad Fazeli Ayda Iravani Parastoo Bayat Akbar Oghalayee Mahshid Mehrabanfar Reza Haj Hosseini Mohammad Tashakorpour Mohsen Tafazzoli Sedigheh Sadeghi |
| author_sort | Zahra Zamani |
| collection | DOAJ |
| description | Background. Colorectal carcinoma is the third cause of cancer deaths in the world. For diagnosis, invasive methods like colonoscopy and sigmoidoscopy are used, and noninvasive screening tests are not very accurate. We decided to study the potential of 1HNMR spectroscopy with metabolomics and chemometrics as a preliminary noninvasive test. We obtained a distinguishing pattern of metabolites and metabolic pathways between colon cancer patient and normal. Methods. Sera were obtained from confirmed colon cancer patients and the same number of healthy controls. Samples were sent for 1HNMR spectroscopy and analysis was carried out Chenomex and MATLAB software. Metabolites were identified using Human Metabolic Data Base (HDMB) and the main metabolic cycles were identified using Metaboanalyst software. Results. 15 metabolites were identified such as pyridoxine, orotidine, and taurocholic acid. Main metabolic cycles involved were the bile acid biosynthesis, vitamin B6 metabolism, methane metabolism, and glutathione metabolism. Discussion. The main detected metabolic cycles were also reported earlier in different cancers. Our observations corroborated earlier studies that suggest the importance of lowering serum LCA/DCA and increasing vitamin B6 intake to help prevent colon cancer. This work can be looked upon as a preliminary step in using 1HNMR analysis as a screening test before invasive procedures. |
| format | Article |
| id | doaj-art-0172a025609f4e6cbafe184d55ccc02f |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-0172a025609f4e6cbafe184d55ccc02f2025-02-03T01:20:22ZengWileyBiochemistry Research International2090-22472090-22552014-01-01201410.1155/2014/348712348712A Metabolic Study on Colon Cancer Using 1H Nuclear Magnetic Resonance SpectroscopyZahra Zamani0Mohammad Arjmand1Farideh Vahabi2Seyed Mahmood Eshaq Hosseini3Sadegh Mohammad Fazeli4Ayda Iravani5Parastoo Bayat6Akbar Oghalayee7Mahshid Mehrabanfar8Reza Haj Hosseini9Mohammad Tashakorpour10Mohsen Tafazzoli11Sedigheh Sadeghi12Biochemistry Department, Pasteur Institute of Iran, Tehran 1316943551, IranBiochemistry Department, Pasteur Institute of Iran, Tehran 1316943551, IranBiochemistry Department, Pasteur Institute of Iran, Tehran 1316943551, IranAmir Alam Hospital, North Sa’adi Avenue, Tehran 8915964665, IranImam Khomeini Hospital, Dr. Gharib Road, Tehran 1419733141, IranBiochemistry Department, Pasteur Institute of Iran, Tehran 1316943551, IranBiochemistry Department, Pasteur Institute of Iran, Tehran 1316943551, IranBiotechnology Department, Pasteur Institute of Iran, Tehran 1316943551, IranPayame Noor University, Tehran 19569, IranPayame Noor University, Tehran 19569, IranAmir Alam Hospital, North Sa’adi Avenue, Tehran 8915964665, IranSharif University, Azadi Avenue, Tehran 11559567, IranBiochemistry Department, Pasteur Institute of Iran, Tehran 1316943551, IranBackground. Colorectal carcinoma is the third cause of cancer deaths in the world. For diagnosis, invasive methods like colonoscopy and sigmoidoscopy are used, and noninvasive screening tests are not very accurate. We decided to study the potential of 1HNMR spectroscopy with metabolomics and chemometrics as a preliminary noninvasive test. We obtained a distinguishing pattern of metabolites and metabolic pathways between colon cancer patient and normal. Methods. Sera were obtained from confirmed colon cancer patients and the same number of healthy controls. Samples were sent for 1HNMR spectroscopy and analysis was carried out Chenomex and MATLAB software. Metabolites were identified using Human Metabolic Data Base (HDMB) and the main metabolic cycles were identified using Metaboanalyst software. Results. 15 metabolites were identified such as pyridoxine, orotidine, and taurocholic acid. Main metabolic cycles involved were the bile acid biosynthesis, vitamin B6 metabolism, methane metabolism, and glutathione metabolism. Discussion. The main detected metabolic cycles were also reported earlier in different cancers. Our observations corroborated earlier studies that suggest the importance of lowering serum LCA/DCA and increasing vitamin B6 intake to help prevent colon cancer. This work can be looked upon as a preliminary step in using 1HNMR analysis as a screening test before invasive procedures.http://dx.doi.org/10.1155/2014/348712 |
| spellingShingle | Zahra Zamani Mohammad Arjmand Farideh Vahabi Seyed Mahmood Eshaq Hosseini Sadegh Mohammad Fazeli Ayda Iravani Parastoo Bayat Akbar Oghalayee Mahshid Mehrabanfar Reza Haj Hosseini Mohammad Tashakorpour Mohsen Tafazzoli Sedigheh Sadeghi A Metabolic Study on Colon Cancer Using 1H Nuclear Magnetic Resonance Spectroscopy Biochemistry Research International |
| title | A Metabolic Study on Colon Cancer Using 1H Nuclear Magnetic Resonance Spectroscopy |
| title_full | A Metabolic Study on Colon Cancer Using 1H Nuclear Magnetic Resonance Spectroscopy |
| title_fullStr | A Metabolic Study on Colon Cancer Using 1H Nuclear Magnetic Resonance Spectroscopy |
| title_full_unstemmed | A Metabolic Study on Colon Cancer Using 1H Nuclear Magnetic Resonance Spectroscopy |
| title_short | A Metabolic Study on Colon Cancer Using 1H Nuclear Magnetic Resonance Spectroscopy |
| title_sort | metabolic study on colon cancer using 1h nuclear magnetic resonance spectroscopy |
| url | http://dx.doi.org/10.1155/2014/348712 |
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