The Role of Clomipramine in Potentiating the Teratogenic Effects of Caffeine in Pregnant Rats: A Histopathological Study
Since little is known about the teratogenic effects of clomipramine used concurrently with caffeine during the organogenesis period, the aim of this study was to test the teratogenic effects of a coadministration of caffeine and clomipramine on rat fetuses. We divided 42 pregnant rats into seven gro...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/382434 |
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| author | Vahid Nikoui Sattar Ostadhadi Nasrin Takzare Seyyed Mohammad-Ali Nabavi Mario Giorgi Azam Bakhtiarian |
| author_facet | Vahid Nikoui Sattar Ostadhadi Nasrin Takzare Seyyed Mohammad-Ali Nabavi Mario Giorgi Azam Bakhtiarian |
| author_sort | Vahid Nikoui |
| collection | DOAJ |
| description | Since little is known about the teratogenic effects of clomipramine used concurrently with caffeine during the organogenesis period, the aim of this study was to test the teratogenic effects of a coadministration of caffeine and clomipramine on rat fetuses. We divided 42 pregnant rats into seven groups, randomly. The first group (control) received 0.5 mL of normal saline. Clomipramine was injected at 40 mg/kg and 80 mg/kg to the second and third groups, respectively. The fourth and fifth groups received caffeine in doses of 60 mg/kg and 120 mg/kg, respectively. The sixth group received a combination of 40 mg/kg clomipramine and 60 mg/kg caffeine, and the seventh group was given clomipramine and caffeine at 80 mg/kg and 120 mg/kg, respectively. The fetuses were removed on the 17th day of pregnancy and studied in terms of microscopic and macroscopic morphological features. Fetuses of rats receiving high doses of caffeine or combinations of caffeine and clomipramine showed a significant rate of cleft palate development, open eyelids, mortality, torsion anomalies, shrinkage of skin, and subcutaneous haemorrhage (P≤0.001). This study concludes that caffeine in high doses or the simultaneous administration of caffeine and clomipramine leads to teratogenicity. |
| format | Article |
| id | doaj-art-016f547d00b74565aee277c460bc9a11 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-016f547d00b74565aee277c460bc9a112025-02-03T01:11:48ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/382434382434The Role of Clomipramine in Potentiating the Teratogenic Effects of Caffeine in Pregnant Rats: A Histopathological StudyVahid Nikoui0Sattar Ostadhadi1Nasrin Takzare2Seyyed Mohammad-Ali Nabavi3Mario Giorgi4Azam Bakhtiarian5Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Keshavarz Boulevard, Tehran 1417613151, IranDepartment of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Keshavarz Boulevard, Tehran 1417613151, IranDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran 1417613146, IranGeneral Physician, School of Medicine, Tehran University of Medical Sciences, Tehran 1417613110, IranDepartment of Veterinary Sciences, University of Pisa, San Piero a Grado, 56122 Pisa, ItalyDepartment of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Keshavarz Boulevard, Tehran 1417613151, IranSince little is known about the teratogenic effects of clomipramine used concurrently with caffeine during the organogenesis period, the aim of this study was to test the teratogenic effects of a coadministration of caffeine and clomipramine on rat fetuses. We divided 42 pregnant rats into seven groups, randomly. The first group (control) received 0.5 mL of normal saline. Clomipramine was injected at 40 mg/kg and 80 mg/kg to the second and third groups, respectively. The fourth and fifth groups received caffeine in doses of 60 mg/kg and 120 mg/kg, respectively. The sixth group received a combination of 40 mg/kg clomipramine and 60 mg/kg caffeine, and the seventh group was given clomipramine and caffeine at 80 mg/kg and 120 mg/kg, respectively. The fetuses were removed on the 17th day of pregnancy and studied in terms of microscopic and macroscopic morphological features. Fetuses of rats receiving high doses of caffeine or combinations of caffeine and clomipramine showed a significant rate of cleft palate development, open eyelids, mortality, torsion anomalies, shrinkage of skin, and subcutaneous haemorrhage (P≤0.001). This study concludes that caffeine in high doses or the simultaneous administration of caffeine and clomipramine leads to teratogenicity.http://dx.doi.org/10.1155/2013/382434 |
| spellingShingle | Vahid Nikoui Sattar Ostadhadi Nasrin Takzare Seyyed Mohammad-Ali Nabavi Mario Giorgi Azam Bakhtiarian The Role of Clomipramine in Potentiating the Teratogenic Effects of Caffeine in Pregnant Rats: A Histopathological Study The Scientific World Journal |
| title | The Role of Clomipramine in Potentiating the Teratogenic Effects of Caffeine in Pregnant Rats: A Histopathological Study |
| title_full | The Role of Clomipramine in Potentiating the Teratogenic Effects of Caffeine in Pregnant Rats: A Histopathological Study |
| title_fullStr | The Role of Clomipramine in Potentiating the Teratogenic Effects of Caffeine in Pregnant Rats: A Histopathological Study |
| title_full_unstemmed | The Role of Clomipramine in Potentiating the Teratogenic Effects of Caffeine in Pregnant Rats: A Histopathological Study |
| title_short | The Role of Clomipramine in Potentiating the Teratogenic Effects of Caffeine in Pregnant Rats: A Histopathological Study |
| title_sort | role of clomipramine in potentiating the teratogenic effects of caffeine in pregnant rats a histopathological study |
| url | http://dx.doi.org/10.1155/2013/382434 |
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