miR-24-3p/FGFR3 Signaling as a Novel Axis Is Involved in Epithelial-Mesenchymal Transition and Regulates Lung Adenocarcinoma Progression
Our previous studies showed that Fibroblast growth factor receptor 3 (FGFR3) contributed to cell growth in lung cancer. However, the correlation between FGFR3 and tumor progression, coupled with the underlying mechanisms, are not fully understood. The clinical significance of FGFR3 was determined in...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/2834109 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551646232576000 |
|---|---|
| author | Pengyu Jing Nan Zhao Nianlin Xie Mingxiang Ye Yong Zhang Zhipei Zhang Mengyang Li Xiaofeng Lai Jian Zhang Zhongping Gu |
| author_facet | Pengyu Jing Nan Zhao Nianlin Xie Mingxiang Ye Yong Zhang Zhipei Zhang Mengyang Li Xiaofeng Lai Jian Zhang Zhongping Gu |
| author_sort | Pengyu Jing |
| collection | DOAJ |
| description | Our previous studies showed that Fibroblast growth factor receptor 3 (FGFR3) contributed to cell growth in lung cancer. However, the correlation between FGFR3 and tumor progression, coupled with the underlying mechanisms, are not fully understood. The clinical significance of FGFR3 was determined in two cohorts of clinical samples (n=22, n=78). A panel of biochemical assays and functional experiments was utilized to elucidate the underlying mechanisms and effects of FGFR3 and miR-24-3p on lung adenocarcinoma progression. Upregulated FGFR3 expression indicated an adverse prognosis for lung adenocarcinoma individuals and promoted metastatic potential of lung adenocarcinoma cells. Owing to the direct regulation towards FGFR3, miR-24-3p could interfere with the potential of proliferation, migration, and invasion in lung adenocarcinoma, following variations of EMT-related protein expression. As a significant marker of EMT, E-cadherin was negatively correlated with FGFR3, of which ectopic overexpression could neutralize the antitumour effects of miR-24-3p and reverse its regulatory effects on EMT markers. Taken together, these findings define a novel insight into the miR-24-3p/FGFR3 signaling axis in regulating lung adenocarcinoma progression and suggest that targeting the miR-24-3p/FGFR3 axis could be an effective and efficient way to prevent tumor progression. |
| format | Article |
| id | doaj-art-01508b504d994f8a8b82211b5da18ed8 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-01508b504d994f8a8b82211b5da18ed82025-02-03T06:00:54ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/28341092834109miR-24-3p/FGFR3 Signaling as a Novel Axis Is Involved in Epithelial-Mesenchymal Transition and Regulates Lung Adenocarcinoma ProgressionPengyu Jing0Nan Zhao1Nianlin Xie2Mingxiang Ye3Yong Zhang4Zhipei Zhang5Mengyang Li6Xiaofeng Lai7Jian Zhang8Zhongping Gu9Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Public Health, Xi’an Medical University, Xi’an 710021, ChinaDepartment of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Pulmonary Medicine, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Pulmonary Medicine, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, ChinaDepartment of Hepato-Biliary and Pancreto-Splenic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, ChinaOur previous studies showed that Fibroblast growth factor receptor 3 (FGFR3) contributed to cell growth in lung cancer. However, the correlation between FGFR3 and tumor progression, coupled with the underlying mechanisms, are not fully understood. The clinical significance of FGFR3 was determined in two cohorts of clinical samples (n=22, n=78). A panel of biochemical assays and functional experiments was utilized to elucidate the underlying mechanisms and effects of FGFR3 and miR-24-3p on lung adenocarcinoma progression. Upregulated FGFR3 expression indicated an adverse prognosis for lung adenocarcinoma individuals and promoted metastatic potential of lung adenocarcinoma cells. Owing to the direct regulation towards FGFR3, miR-24-3p could interfere with the potential of proliferation, migration, and invasion in lung adenocarcinoma, following variations of EMT-related protein expression. As a significant marker of EMT, E-cadherin was negatively correlated with FGFR3, of which ectopic overexpression could neutralize the antitumour effects of miR-24-3p and reverse its regulatory effects on EMT markers. Taken together, these findings define a novel insight into the miR-24-3p/FGFR3 signaling axis in regulating lung adenocarcinoma progression and suggest that targeting the miR-24-3p/FGFR3 axis could be an effective and efficient way to prevent tumor progression.http://dx.doi.org/10.1155/2018/2834109 |
| spellingShingle | Pengyu Jing Nan Zhao Nianlin Xie Mingxiang Ye Yong Zhang Zhipei Zhang Mengyang Li Xiaofeng Lai Jian Zhang Zhongping Gu miR-24-3p/FGFR3 Signaling as a Novel Axis Is Involved in Epithelial-Mesenchymal Transition and Regulates Lung Adenocarcinoma Progression Journal of Immunology Research |
| title | miR-24-3p/FGFR3 Signaling as a Novel Axis Is Involved in Epithelial-Mesenchymal Transition and Regulates Lung Adenocarcinoma Progression |
| title_full | miR-24-3p/FGFR3 Signaling as a Novel Axis Is Involved in Epithelial-Mesenchymal Transition and Regulates Lung Adenocarcinoma Progression |
| title_fullStr | miR-24-3p/FGFR3 Signaling as a Novel Axis Is Involved in Epithelial-Mesenchymal Transition and Regulates Lung Adenocarcinoma Progression |
| title_full_unstemmed | miR-24-3p/FGFR3 Signaling as a Novel Axis Is Involved in Epithelial-Mesenchymal Transition and Regulates Lung Adenocarcinoma Progression |
| title_short | miR-24-3p/FGFR3 Signaling as a Novel Axis Is Involved in Epithelial-Mesenchymal Transition and Regulates Lung Adenocarcinoma Progression |
| title_sort | mir 24 3p fgfr3 signaling as a novel axis is involved in epithelial mesenchymal transition and regulates lung adenocarcinoma progression |
| url | http://dx.doi.org/10.1155/2018/2834109 |
| work_keys_str_mv | AT pengyujing mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression AT nanzhao mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression AT nianlinxie mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression AT mingxiangye mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression AT yongzhang mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression AT zhipeizhang mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression AT mengyangli mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression AT xiaofenglai mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression AT jianzhang mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression AT zhongpinggu mir243pfgfr3signalingasanovelaxisisinvolvedinepithelialmesenchymaltransitionandregulateslungadenocarcinomaprogression |