Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling
Calcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/β-tricalcium phosphate (TCP) and confirmed their biological activity in vitro and bone...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/507154 |
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| author | Xuehui Zhang Song Meng Ying Huang Mingming Xu Ying He Hong Lin Jianmin Han Yuan Chai Yan Wei Xuliang Deng |
| author_facet | Xuehui Zhang Song Meng Ying Huang Mingming Xu Ying He Hong Lin Jianmin Han Yuan Chai Yan Wei Xuliang Deng |
| author_sort | Xuehui Zhang |
| collection | DOAJ |
| description | Calcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/β-tricalcium phosphate (TCP) and confirmed their biological activity in vitro and bone regeneration in vivo. However, how these composite nanofibers promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is unknown. Here, gelatin/β-TCP composite nanofibers were fabricated by incorporating 20 wt% β-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the composite β-TCP nanofibers had a nonwoven structure with a porous network and a rough surface. Spectral analyses confirmed the presence and chemical stability of the β-TCP and gelatin components. Compared with pure gelatin nanofibers, gelatin/β-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs. Interestingly, the expression level of the calcium-sensing receptor (CaSR) was significantly higher on the composite nanofibrous scaffolds than on pure gelatin. For rat calvarial critical sized defects, more extensive osteogenesis and neovascularization occurred in the composite scaffolds group compared with the gelatin group. Thus, gelatin/β-TCP composite scaffolds promote osteogenic differentiation of BMSCs in vitro and bone regeneration in vivo by activating Ca2+-sensing receptor signaling. |
| format | Article |
| id | doaj-art-0145b8b9da4a439aafcadaf4ec668e2d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0145b8b9da4a439aafcadaf4ec668e2d2025-02-03T06:13:43ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/507154507154Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor SignalingXuehui Zhang0Song Meng1Ying Huang2Mingming Xu3Ying He4Hong Lin5Jianmin Han6Yuan Chai7Yan Wei8Xuliang Deng9Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Dental Materials, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Dental Materials, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Dental Materials, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaCalcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/β-tricalcium phosphate (TCP) and confirmed their biological activity in vitro and bone regeneration in vivo. However, how these composite nanofibers promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is unknown. Here, gelatin/β-TCP composite nanofibers were fabricated by incorporating 20 wt% β-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the composite β-TCP nanofibers had a nonwoven structure with a porous network and a rough surface. Spectral analyses confirmed the presence and chemical stability of the β-TCP and gelatin components. Compared with pure gelatin nanofibers, gelatin/β-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs. Interestingly, the expression level of the calcium-sensing receptor (CaSR) was significantly higher on the composite nanofibrous scaffolds than on pure gelatin. For rat calvarial critical sized defects, more extensive osteogenesis and neovascularization occurred in the composite scaffolds group compared with the gelatin group. Thus, gelatin/β-TCP composite scaffolds promote osteogenic differentiation of BMSCs in vitro and bone regeneration in vivo by activating Ca2+-sensing receptor signaling.http://dx.doi.org/10.1155/2015/507154 |
| spellingShingle | Xuehui Zhang Song Meng Ying Huang Mingming Xu Ying He Hong Lin Jianmin Han Yuan Chai Yan Wei Xuliang Deng Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling Stem Cells International |
| title | Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling |
| title_full | Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling |
| title_fullStr | Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling |
| title_full_unstemmed | Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling |
| title_short | Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling |
| title_sort | electrospun gelatin β tcp composite nanofibers enhance osteogenic differentiation of bmscs and in vivo bone formation by activating ca2 sensing receptor signaling |
| url | http://dx.doi.org/10.1155/2015/507154 |
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