An innovative method used for the identification of N-glycans on soybean allergen β-conglycinin
β-Conglycinin is one of the major allergens existed in soybean. N-Glycans attached to the β-conglycinin influenced the immunoreactivity and antigen presenting efficiency of β-conglycinin. In this study, we described a new method used to release and collect the N-glycans from β-conglycinin, and the N...
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| Format: | Article |
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Tsinghua University Press
2023-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002129 |
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| author | Cheng Li Yang Tian Jianli Han Yu Lu Meiyi Zou Yue Jia Chengjian Wang Linjuan Huang Zhongfu Wang |
| author_facet | Cheng Li Yang Tian Jianli Han Yu Lu Meiyi Zou Yue Jia Chengjian Wang Linjuan Huang Zhongfu Wang |
| author_sort | Cheng Li |
| collection | DOAJ |
| description | β-Conglycinin is one of the major allergens existed in soybean. N-Glycans attached to the β-conglycinin influenced the immunoreactivity and antigen presenting efficiency of β-conglycinin. In this study, we described a new method used to release and collect the N-glycans from β-conglycinin, and the N-glycans existed in linear epitopes of β-conglycinin were identified. Glycopeptides hydrolyzed from β-conglycinin were purified by cotton hydrophilic chromatography. Trifluoromethylsulfonic acid was then used to release glycans from glycopeptides, and new glycopeptides containing one single N-acetyl-D-glucosamine (GlcNAc) moiety were then utilized for mass spectrometry. Five glycosylation sites (Asn-199, Asn-455, Asn-215, Asn-489 and Asn-326) and 22 kinds of glycopeptides were identified. It is noteworthy that the peptide VVN#ATSNL (where # represents for the glycosylation site) was analyzed to be both glycopeptide and linear epitope. Our results provided a new method for the N-glycoform analysis of food allergens, and laid a foundation for understanding the relationship between glycosylation and food allergy. |
| format | Article |
| id | doaj-art-014403ef70254ca5a0c9181e5550ac9d |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-014403ef70254ca5a0c9181e5550ac9d2025-02-03T06:53:35ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123842850An innovative method used for the identification of N-glycans on soybean allergen β-conglycininCheng Li0Yang Tian1Jianli Han2Yu Lu3Meiyi Zou4Yue Jia5Chengjian Wang6Linjuan Huang7Zhongfu Wang8Key Laboratory of Glycobiology and Glycoengineering of Xi’an, College of Food Science and Technology, Northwest University, Xi’an 710069, ChinaKey Laboratory of Glycobiology and Glycoengineering of Xi’an, College of Food Science and Technology, Northwest University, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, Xi’an 710069, ChinaKey Laboratory of Glycobiology and Glycoengineering of Xi’an, College of Food Science and Technology, Northwest University, Xi’an 710069, ChinaKey Laboratory of Glycobiology and Glycoengineering of Xi’an, College of Food Science and Technology, Northwest University, Xi’an 710069, China; College of Life Sciences, Northwest University, Xi’an 710069, China; Corresponding author at: College of Food Science and Technology, Northwest University, Xi’an 710069, China. Fax: +86 29 88373079Key Laboratory of Glycobiology and Glycoengineering of Xi’an, College of Food Science and Technology, Northwest University, Xi’an 710069, China; College of Life Sciences, Northwest University, Xi’an 710069, China; Corresponding author at: College of Food Science and Technology, Northwest University, Xi’an 710069, China. Fax: +86 29 88373079β-Conglycinin is one of the major allergens existed in soybean. N-Glycans attached to the β-conglycinin influenced the immunoreactivity and antigen presenting efficiency of β-conglycinin. In this study, we described a new method used to release and collect the N-glycans from β-conglycinin, and the N-glycans existed in linear epitopes of β-conglycinin were identified. Glycopeptides hydrolyzed from β-conglycinin were purified by cotton hydrophilic chromatography. Trifluoromethylsulfonic acid was then used to release glycans from glycopeptides, and new glycopeptides containing one single N-acetyl-D-glucosamine (GlcNAc) moiety were then utilized for mass spectrometry. Five glycosylation sites (Asn-199, Asn-455, Asn-215, Asn-489 and Asn-326) and 22 kinds of glycopeptides were identified. It is noteworthy that the peptide VVN#ATSNL (where # represents for the glycosylation site) was analyzed to be both glycopeptide and linear epitope. Our results provided a new method for the N-glycoform analysis of food allergens, and laid a foundation for understanding the relationship between glycosylation and food allergy.http://www.sciencedirect.com/science/article/pii/S2213453022002129Soybean allergen β-conglycininGlycopeptideMass spectrometryN-GlycanGlycosylation site |
| spellingShingle | Cheng Li Yang Tian Jianli Han Yu Lu Meiyi Zou Yue Jia Chengjian Wang Linjuan Huang Zhongfu Wang An innovative method used for the identification of N-glycans on soybean allergen β-conglycinin Food Science and Human Wellness Soybean allergen β-conglycinin Glycopeptide Mass spectrometry N-Glycan Glycosylation site |
| title | An innovative method used for the identification of N-glycans on soybean allergen β-conglycinin |
| title_full | An innovative method used for the identification of N-glycans on soybean allergen β-conglycinin |
| title_fullStr | An innovative method used for the identification of N-glycans on soybean allergen β-conglycinin |
| title_full_unstemmed | An innovative method used for the identification of N-glycans on soybean allergen β-conglycinin |
| title_short | An innovative method used for the identification of N-glycans on soybean allergen β-conglycinin |
| title_sort | innovative method used for the identification of n glycans on soybean allergen β conglycinin |
| topic | Soybean allergen β-conglycinin Glycopeptide Mass spectrometry N-Glycan Glycosylation site |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002129 |
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