Combining genotyping approaches improves resolution for association mapping: a case study in tropical maize under water stress conditions
Genome-wide Association Studies (GWAS) identify genome variations related to specific phenotypes using Single Nucleotide Polymorphism (SNP) markers. Genotyping platforms like SNP-Array or sequencing-based techniques (GBS) can genotype samples with many SNPs. These approaches may bias tropical maize...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1442008/full |
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| author | Fernanda Carla Ferreira de Pontes Ingrid Pinheiro Machado Maria Valnice de Souza Silveira Antônio Lucas Aguiar Lobo Felipe Sabadin Roberto Fritsche-Neto Júlio César DoVale |
| author_facet | Fernanda Carla Ferreira de Pontes Ingrid Pinheiro Machado Maria Valnice de Souza Silveira Antônio Lucas Aguiar Lobo Felipe Sabadin Roberto Fritsche-Neto Júlio César DoVale |
| author_sort | Fernanda Carla Ferreira de Pontes |
| collection | DOAJ |
| description | Genome-wide Association Studies (GWAS) identify genome variations related to specific phenotypes using Single Nucleotide Polymorphism (SNP) markers. Genotyping platforms like SNP-Array or sequencing-based techniques (GBS) can genotype samples with many SNPs. These approaches may bias tropical maize analyses due to reliance on the temperate line B73 as the reference genome. An alternative is a simulated genome called “Mock,” adapted to the population using bioinformatics. Recent studies show SNP-Array, GBS, and Mock yield similar results for population structure, heterotic groups definition, tester selection, and genomic hybrid prediction. However, no studies have examined the results generated by these different genotyping approaches for GWAS. This study aims to test the equivalence among the three genotyping scenarios in identifying significant effect genes in GWAS. To achieve this, maize was used as the model species, where SNP-Array genotyped 360 inbred lines from a public panel via the Affymetrix platform and GBS. The GBS data were used to perform SNP calling using the temperate inbred line B73 as the reference genome (GBS-B73) and a simulated genome “Mock” obtained in-silico (GBS-Mock). The study encompassed four above-ground traits with plants grown under two levels of water supply: well-watered (WW) and water-stressed (WS). In total, 46, 34, and 31 SNP were identified in the SNP-Array, GBS-B73, and GBS-Mock scenarios, respectively, across the two water levels, associated with the evaluated traits following the comparative analysis of each genotyping method individually. Overall, the identified candidate genes varied along the various scenarios but had the same functionality. Regarding SNP-Array and GBS-B73, genes with functional similarity were identified even without coincidence in the physical position of the SNPs. These genes and regions are involved in various processes and responses with applications in plant breeding. In terms of accuracy, the combination of genotyping scenarios compared to those isolated is feasible and recommended, as it increased all traits under both water conditions. In this sense, it is worth highlighting the combination of GBS-B73 and GBS-Mock scenarios, not only due to the increase in the resolution of GWAS results but also the reduction of costs associated with genotyping and the possibility of conducting genomic breeding methods. |
| format | Article |
| id | doaj-art-0143a57705ff4cddb0ca64a684f7ccae |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-0143a57705ff4cddb0ca64a684f7ccae2025-01-23T06:56:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.14420081442008Combining genotyping approaches improves resolution for association mapping: a case study in tropical maize under water stress conditionsFernanda Carla Ferreira de Pontes0Ingrid Pinheiro Machado1Maria Valnice de Souza Silveira2Antônio Lucas Aguiar Lobo3Felipe Sabadin4Roberto Fritsche-Neto5Júlio César DoVale6Postgraduate Program of Plant Science, Federal University of Ceará, Fortaleza, Ceará, BrazilPostgraduate Program of Plant Science, Federal University of Ceará, Fortaleza, Ceará, BrazilPostgraduate Program of Plant Science, Federal University of Ceará, Fortaleza, Ceará, BrazilPostgraduate Program of Plant Science, Federal University of Ceará, Fortaleza, Ceará, BrazilCollege of Agriculture and Applied Sciences, Utah State University, Logan, UT, United StatesLouisiana State University Agricultural Center, Baton Rouge, LA, United StatesPostgraduate Program of Plant Science, Federal University of Ceará, Fortaleza, Ceará, BrazilGenome-wide Association Studies (GWAS) identify genome variations related to specific phenotypes using Single Nucleotide Polymorphism (SNP) markers. Genotyping platforms like SNP-Array or sequencing-based techniques (GBS) can genotype samples with many SNPs. These approaches may bias tropical maize analyses due to reliance on the temperate line B73 as the reference genome. An alternative is a simulated genome called “Mock,” adapted to the population using bioinformatics. Recent studies show SNP-Array, GBS, and Mock yield similar results for population structure, heterotic groups definition, tester selection, and genomic hybrid prediction. However, no studies have examined the results generated by these different genotyping approaches for GWAS. This study aims to test the equivalence among the three genotyping scenarios in identifying significant effect genes in GWAS. To achieve this, maize was used as the model species, where SNP-Array genotyped 360 inbred lines from a public panel via the Affymetrix platform and GBS. The GBS data were used to perform SNP calling using the temperate inbred line B73 as the reference genome (GBS-B73) and a simulated genome “Mock” obtained in-silico (GBS-Mock). The study encompassed four above-ground traits with plants grown under two levels of water supply: well-watered (WW) and water-stressed (WS). In total, 46, 34, and 31 SNP were identified in the SNP-Array, GBS-B73, and GBS-Mock scenarios, respectively, across the two water levels, associated with the evaluated traits following the comparative analysis of each genotyping method individually. Overall, the identified candidate genes varied along the various scenarios but had the same functionality. Regarding SNP-Array and GBS-B73, genes with functional similarity were identified even without coincidence in the physical position of the SNPs. These genes and regions are involved in various processes and responses with applications in plant breeding. In terms of accuracy, the combination of genotyping scenarios compared to those isolated is feasible and recommended, as it increased all traits under both water conditions. In this sense, it is worth highlighting the combination of GBS-B73 and GBS-Mock scenarios, not only due to the increase in the resolution of GWAS results but also the reduction of costs associated with genotyping and the possibility of conducting genomic breeding methods.https://www.frontiersin.org/articles/10.3389/fpls.2024.1442008/fullSNP-arraygenotyping by sequencingsimulated genomeGWASgenotyping platformscandidate genes |
| spellingShingle | Fernanda Carla Ferreira de Pontes Ingrid Pinheiro Machado Maria Valnice de Souza Silveira Antônio Lucas Aguiar Lobo Felipe Sabadin Roberto Fritsche-Neto Júlio César DoVale Combining genotyping approaches improves resolution for association mapping: a case study in tropical maize under water stress conditions Frontiers in Plant Science SNP-array genotyping by sequencing simulated genome GWAS genotyping platforms candidate genes |
| title | Combining genotyping approaches improves resolution for association mapping: a case study in tropical maize under water stress conditions |
| title_full | Combining genotyping approaches improves resolution for association mapping: a case study in tropical maize under water stress conditions |
| title_fullStr | Combining genotyping approaches improves resolution for association mapping: a case study in tropical maize under water stress conditions |
| title_full_unstemmed | Combining genotyping approaches improves resolution for association mapping: a case study in tropical maize under water stress conditions |
| title_short | Combining genotyping approaches improves resolution for association mapping: a case study in tropical maize under water stress conditions |
| title_sort | combining genotyping approaches improves resolution for association mapping a case study in tropical maize under water stress conditions |
| topic | SNP-array genotyping by sequencing simulated genome GWAS genotyping platforms candidate genes |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1442008/full |
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