Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real-Time Polymerase Chain Reaction

BACKGROUND: Bordetella pertussis infections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detect B pertussis are typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificit...

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Main Authors: Mohammad Rubayet Hasan, Rusung Tan, Ghada N Al-Rawahi, Eva Thomas, Peter Tilley
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:Canadian Journal of Infectious Diseases and Medical Microbiology
Online Access:http://dx.doi.org/10.1155/2014/763128
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author Mohammad Rubayet Hasan
Rusung Tan
Ghada N Al-Rawahi
Eva Thomas
Peter Tilley
author_facet Mohammad Rubayet Hasan
Rusung Tan
Ghada N Al-Rawahi
Eva Thomas
Peter Tilley
author_sort Mohammad Rubayet Hasan
collection DOAJ
description BACKGROUND: Bordetella pertussis infections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detect B pertussis are typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificity.
format Article
id doaj-art-01393c6063254734893e87ac11b1bf35
institution Kabale University
issn 1712-9532
language English
publishDate 2014-01-01
publisher Wiley
record_format Article
series Canadian Journal of Infectious Diseases and Medical Microbiology
spelling doaj-art-01393c6063254734893e87ac11b1bf352025-02-03T05:44:18ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95322014-01-0125421722110.1155/2014/763128Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real-Time Polymerase Chain ReactionMohammad Rubayet Hasan0Rusung Tan1Ghada N Al-Rawahi2Eva Thomas3Peter Tilley4Division of Microbiology, Virology and Infection Control, Department of Pathology, Children’s and Women’s Health Centre of BC, CanadaDivision of Microbiology, Virology and Infection Control, Department of Pathology, Children’s and Women’s Health Centre of BC, CanadaDivision of Microbiology, Virology and Infection Control, Department of Pathology, Children’s and Women’s Health Centre of BC, CanadaDivision of Microbiology, Virology and Infection Control, Department of Pathology, Children’s and Women’s Health Centre of BC, CanadaDivision of Microbiology, Virology and Infection Control, Department of Pathology, Children’s and Women’s Health Centre of BC, CanadaBACKGROUND: Bordetella pertussis infections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detect B pertussis are typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificity.http://dx.doi.org/10.1155/2014/763128
spellingShingle Mohammad Rubayet Hasan
Rusung Tan
Ghada N Al-Rawahi
Eva Thomas
Peter Tilley
Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real-Time Polymerase Chain Reaction
Canadian Journal of Infectious Diseases and Medical Microbiology
title Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real-Time Polymerase Chain Reaction
title_full Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real-Time Polymerase Chain Reaction
title_fullStr Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real-Time Polymerase Chain Reaction
title_full_unstemmed Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real-Time Polymerase Chain Reaction
title_short Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real-Time Polymerase Chain Reaction
title_sort evaluation of amplification targets for the specific detection of bordetella pertussis using real time polymerase chain reaction
url http://dx.doi.org/10.1155/2014/763128
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AT ghadanalrawahi evaluationofamplificationtargetsforthespecificdetectionofbordetellapertussisusingrealtimepolymerasechainreaction
AT evathomas evaluationofamplificationtargetsforthespecificdetectionofbordetellapertussisusingrealtimepolymerasechainreaction
AT petertilley evaluationofamplificationtargetsforthespecificdetectionofbordetellapertussisusingrealtimepolymerasechainreaction