Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis
Pericytes are a population of cells that participate in normal vessel architecture and regulate permeability. Apelin, as the endogenous ligand of G protein-coupled receptor APJ, participates in a number of physiological and pathological processes. To date, the effect of apelin on pericyte is not cle...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2015/186946 |
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| author | Li Chen Yong Tao Jing Feng Yan Rong Jiang |
| author_facet | Li Chen Yong Tao Jing Feng Yan Rong Jiang |
| author_sort | Li Chen |
| collection | DOAJ |
| description | Pericytes are a population of cells that participate in normal vessel architecture and regulate permeability. Apelin, as the endogenous ligand of G protein-coupled receptor APJ, participates in a number of physiological and pathological processes. To date, the effect of apelin on pericyte is not clear. Our study aimed to investigate the potential protection mechanisms of apelin, with regard to primary rat retinal pericytes under hypoxia. Immunofluorescence staining revealed that pericytes colocalized with APJ in the fibrovascular membranes dissected from proliferative diabetic retinopathy patients. In the in vitro studies, we first demonstrated that the expression of apelin/APJ was upregulated in pericytes under hypoxia, and apelin increased pericytes proliferation and migration. Moreover, knockdown of apelin in pericyte was achieved via lentivirus-mediated RNA interference. After the inhibition of apelin, pericytes proliferation was inhibited significantly in hypoxia culture condition. Furthermore, exogenous recombinant apelin effectively prevented hypoxia-induced apoptosis through downregulating active-caspase 3 expression and increasing the ratio of B cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax) in pericytes. These results suggest that apelin suppressed hypoxia-induced pericytes injury, which indicated that apelin could be a potential therapeutic target for retinal angiogenic diseases. |
| format | Article |
| id | doaj-art-01269da5e4b349b6b94aa3e1ad3d10fa |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-01269da5e4b349b6b94aa3e1ad3d10fa2025-02-03T05:58:44ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/186946186946Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced ApoptosisLi Chen0Yong Tao1Jing Feng2Yan Rong Jiang3Department of Ophthalmology, People’s Hospital, Peking University, 11 Xizhimen South Street, Xicheng District, Beijing 100044, ChinaDepartment of Ophthalmology, People’s Hospital, Peking University, 11 Xizhimen South Street, Xicheng District, Beijing 100044, ChinaDepartment of Ophthalmology, People’s Hospital, Peking University, 11 Xizhimen South Street, Xicheng District, Beijing 100044, ChinaDepartment of Ophthalmology, People’s Hospital, Peking University, 11 Xizhimen South Street, Xicheng District, Beijing 100044, ChinaPericytes are a population of cells that participate in normal vessel architecture and regulate permeability. Apelin, as the endogenous ligand of G protein-coupled receptor APJ, participates in a number of physiological and pathological processes. To date, the effect of apelin on pericyte is not clear. Our study aimed to investigate the potential protection mechanisms of apelin, with regard to primary rat retinal pericytes under hypoxia. Immunofluorescence staining revealed that pericytes colocalized with APJ in the fibrovascular membranes dissected from proliferative diabetic retinopathy patients. In the in vitro studies, we first demonstrated that the expression of apelin/APJ was upregulated in pericytes under hypoxia, and apelin increased pericytes proliferation and migration. Moreover, knockdown of apelin in pericyte was achieved via lentivirus-mediated RNA interference. After the inhibition of apelin, pericytes proliferation was inhibited significantly in hypoxia culture condition. Furthermore, exogenous recombinant apelin effectively prevented hypoxia-induced apoptosis through downregulating active-caspase 3 expression and increasing the ratio of B cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax) in pericytes. These results suggest that apelin suppressed hypoxia-induced pericytes injury, which indicated that apelin could be a potential therapeutic target for retinal angiogenic diseases.http://dx.doi.org/10.1155/2015/186946 |
| spellingShingle | Li Chen Yong Tao Jing Feng Yan Rong Jiang Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis Journal of Ophthalmology |
| title | Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis |
| title_full | Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis |
| title_fullStr | Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis |
| title_full_unstemmed | Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis |
| title_short | Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis |
| title_sort | apelin protects primary rat retinal pericytes from chemical hypoxia induced apoptosis |
| url | http://dx.doi.org/10.1155/2015/186946 |
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