Regulation of electrolyte transport with IL-1β in rabbit distal colon
Interletrkin-1β levels are elevated in inflammatory bowel disease. In this study the mechanism by which interleukin-1β affects electrolyte transport in the rabbit distal colon, was investigated. Interleukin-1β caused a delayed increase in short-circuit current (Isc) which was attributed to protein s...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000111 |
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| _version_ | 1832562233227345920 |
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| author | F. R. Homaidan H. Desai L. Zhao G. Broutman R. Burakoff |
| author_facet | F. R. Homaidan H. Desai L. Zhao G. Broutman R. Burakoff |
| author_sort | F. R. Homaidan |
| collection | DOAJ |
| description | Interletrkin-1β levels are elevated in inflammatory bowel disease. In this study the mechanism by which interleukin-1β affects electrolyte transport in the rabbit distal colon, was investigated. Interleukin-1β caused a delayed increase in short-circuit current (Isc) which was attributed to protein synthesis since the effect was inhibited by cycloheximide. The interleukin-1β induced increase in Isc was not affected by amiloride treatment but was completely inhibited by bumetanide or in chloride-free buffer and by indomethacin. Prostaglandin E2 levels increased in tissue treated with interleukin-1β, but this increase was reversed by cycloheximide. These data suggest that interleukin-1β causes its effect via a yet to be identified second messenger, by increasing chloride secretion through a prostaglandin E2 mediated mechanism. |
| format | Article |
| id | doaj-art-0115f569b3cd4c7b8d8ff6b4fd626f5d |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0115f569b3cd4c7b8d8ff6b4fd626f5d2025-02-03T01:23:07ZengWileyMediators of Inflammation0962-93511466-18611995-01-0141616610.1155/S0962935195000111Regulation of electrolyte transport with IL-1β in rabbit distal colonF. R. Homaidan0H. Desai1L. Zhao2G. Broutman3R. Burakoff4Division of Gastroenterology and Nutrition, Winthrop-University Hospital , Mineola, NY 11501, USADivision of Gastroenterology and Nutrition, Winthrop-University Hospital , Mineola, NY 11501, USADivision of Gastroenterology and Nutrition, Winthrop-University Hospital , Mineola, NY 11501, USADivision of Gastroenterology and Nutrition, Winthrop-University Hospital , Mineola, NY 11501, USADivision of Gastroenterology and Nutrition, Winthrop-University Hospital , Mineola, NY 11501, USAInterletrkin-1β levels are elevated in inflammatory bowel disease. In this study the mechanism by which interleukin-1β affects electrolyte transport in the rabbit distal colon, was investigated. Interleukin-1β caused a delayed increase in short-circuit current (Isc) which was attributed to protein synthesis since the effect was inhibited by cycloheximide. The interleukin-1β induced increase in Isc was not affected by amiloride treatment but was completely inhibited by bumetanide or in chloride-free buffer and by indomethacin. Prostaglandin E2 levels increased in tissue treated with interleukin-1β, but this increase was reversed by cycloheximide. These data suggest that interleukin-1β causes its effect via a yet to be identified second messenger, by increasing chloride secretion through a prostaglandin E2 mediated mechanism.http://dx.doi.org/10.1155/S0962935195000111 |
| spellingShingle | F. R. Homaidan H. Desai L. Zhao G. Broutman R. Burakoff Regulation of electrolyte transport with IL-1β in rabbit distal colon Mediators of Inflammation |
| title | Regulation of electrolyte transport with IL-1β in rabbit distal colon |
| title_full | Regulation of electrolyte transport with IL-1β in rabbit distal colon |
| title_fullStr | Regulation of electrolyte transport with IL-1β in rabbit distal colon |
| title_full_unstemmed | Regulation of electrolyte transport with IL-1β in rabbit distal colon |
| title_short | Regulation of electrolyte transport with IL-1β in rabbit distal colon |
| title_sort | regulation of electrolyte transport with il 1β in rabbit distal colon |
| url | http://dx.doi.org/10.1155/S0962935195000111 |
| work_keys_str_mv | AT frhomaidan regulationofelectrolytetransportwithil1binrabbitdistalcolon AT hdesai regulationofelectrolytetransportwithil1binrabbitdistalcolon AT lzhao regulationofelectrolytetransportwithil1binrabbitdistalcolon AT gbroutman regulationofelectrolytetransportwithil1binrabbitdistalcolon AT rburakoff regulationofelectrolytetransportwithil1binrabbitdistalcolon |