Enhanced meropenem activity and stability following load in Polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on Pseudomonas aeruginosa
Abstract Background The increasing resistance of bacteria to conventional antibiotics poses a significant health challenge. Innovative strategies, such as combining antibiotics with agents like quorum sensing inhibitors (QSIs), have been developed to combat this issue. QSIs enhance antibiotic effica...
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2025-08-01
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| Online Access: | https://doi.org/10.1186/s13036-025-00549-1 |
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| author | Abdelrahman A. Mohsen Taghrid S. El-Mahdy Mohamed Emara Samar A. Salim |
| author_facet | Abdelrahman A. Mohsen Taghrid S. El-Mahdy Mohamed Emara Samar A. Salim |
| author_sort | Abdelrahman A. Mohsen |
| collection | DOAJ |
| description | Abstract Background The increasing resistance of bacteria to conventional antibiotics poses a significant health challenge. Innovative strategies, such as combining antibiotics with agents like quorum sensing inhibitors (QSIs), have been developed to combat this issue. QSIs enhance antibiotic efficacy without inhibiting bacterial growth, minimizing the risk of resistance. Aims Evaluate the combined effect of Sitagliptin (STG) as a QSI with Meropenem (MER), fabricate drug-loaded Polyvinyl Alcohol (PVA) nanofibers, and investigate their antimicrobial activity against standard Pseudomonas aeruginosa (PAO1) and carbapenem-resistant P. aeruginosa (CRPA). Methods The combinatorial effect was assessed using a checkerboard assay. PVA/STG, PVA/MER, and PVA/STG/MER nanofibers were fabricated with varying concentrations of STG (2, 4, 8 mg/mL) and MER (5, 7, 9 mg/mL) via electrospinning. Characterization was performed using FTIR, XRD, and SEM techniques. Results STG significantly reduced the minimum inhibitory concentration (MIC) of MER. The 1:2 STG to MER ratio exhibited the highest antimicrobial activity, achieving a comparable zone of inhibition to the highest concentration of MER while utilizing nearly half the amount of MER. The stability of the loaded scaffolds was maintained over three months at 2–8 °C. Conclusions Our results underscore the successful fabrication of nanofiber scaffolds and the effectiveness of STG and MER-loaded nanofibers as promising wound dressings for cutaneous P. aeruginosa infections. This study highlights the potential of our innovative nanofiber system to enhance treatment efficacy against multidrug-resistant bacteria, offering a personalized and rapid response wound dressing solution for medical professionals. Ultimately, it shows promise to improve patient recovery and quality of life while minimizing systemic side effects. |
| format | Article |
| id | doaj-art-0109df2fe74c4e2f93c700a1e547b2bc |
| institution | Kabale University |
| issn | 1754-1611 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj-art-0109df2fe74c4e2f93c700a1e547b2bc2025-08-24T11:34:06ZengBMCJournal of Biological Engineering1754-16112025-08-0119111210.1186/s13036-025-00549-1Enhanced meropenem activity and stability following load in Polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on Pseudomonas aeruginosaAbdelrahman A. Mohsen0Taghrid S. El-Mahdy1Mohamed Emara2Samar A. Salim3Department of Microbiology and Immunology, Faculty of pharmacy, Helwan university-Ain HelwanDepartment of Microbiology and Immunology, Faculty of pharmacy, Helwan university-Ain HelwanDepartment of Microbiology and Immunology, Faculty of pharmacy, Helwan university-Ain HelwanNanotechnology Research Center (NTRC), The British University in Egypt (BUE)Abstract Background The increasing resistance of bacteria to conventional antibiotics poses a significant health challenge. Innovative strategies, such as combining antibiotics with agents like quorum sensing inhibitors (QSIs), have been developed to combat this issue. QSIs enhance antibiotic efficacy without inhibiting bacterial growth, minimizing the risk of resistance. Aims Evaluate the combined effect of Sitagliptin (STG) as a QSI with Meropenem (MER), fabricate drug-loaded Polyvinyl Alcohol (PVA) nanofibers, and investigate their antimicrobial activity against standard Pseudomonas aeruginosa (PAO1) and carbapenem-resistant P. aeruginosa (CRPA). Methods The combinatorial effect was assessed using a checkerboard assay. PVA/STG, PVA/MER, and PVA/STG/MER nanofibers were fabricated with varying concentrations of STG (2, 4, 8 mg/mL) and MER (5, 7, 9 mg/mL) via electrospinning. Characterization was performed using FTIR, XRD, and SEM techniques. Results STG significantly reduced the minimum inhibitory concentration (MIC) of MER. The 1:2 STG to MER ratio exhibited the highest antimicrobial activity, achieving a comparable zone of inhibition to the highest concentration of MER while utilizing nearly half the amount of MER. The stability of the loaded scaffolds was maintained over three months at 2–8 °C. Conclusions Our results underscore the successful fabrication of nanofiber scaffolds and the effectiveness of STG and MER-loaded nanofibers as promising wound dressings for cutaneous P. aeruginosa infections. This study highlights the potential of our innovative nanofiber system to enhance treatment efficacy against multidrug-resistant bacteria, offering a personalized and rapid response wound dressing solution for medical professionals. Ultimately, it shows promise to improve patient recovery and quality of life while minimizing systemic side effects.https://doi.org/10.1186/s13036-025-00549-1NanofibersSitagliptinMeropenemAntibacterial activityElectrospinner |
| spellingShingle | Abdelrahman A. Mohsen Taghrid S. El-Mahdy Mohamed Emara Samar A. Salim Enhanced meropenem activity and stability following load in Polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on Pseudomonas aeruginosa Journal of Biological Engineering Nanofibers Sitagliptin Meropenem Antibacterial activity Electrospinner |
| title | Enhanced meropenem activity and stability following load in Polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on Pseudomonas aeruginosa |
| title_full | Enhanced meropenem activity and stability following load in Polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on Pseudomonas aeruginosa |
| title_fullStr | Enhanced meropenem activity and stability following load in Polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on Pseudomonas aeruginosa |
| title_full_unstemmed | Enhanced meropenem activity and stability following load in Polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on Pseudomonas aeruginosa |
| title_short | Enhanced meropenem activity and stability following load in Polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on Pseudomonas aeruginosa |
| title_sort | enhanced meropenem activity and stability following load in polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on pseudomonas aeruginosa |
| topic | Nanofibers Sitagliptin Meropenem Antibacterial activity Electrospinner |
| url | https://doi.org/10.1186/s13036-025-00549-1 |
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